ID   SUHA_CAVPO              Reviewed;         287 AA.
AC   P50234;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=3-alpha-hydroxysteroid sulfotransferase;
DE            EC=2.8.2.2;
DE   AltName: Full=Alcohol sulfotransferase;
DE   AltName: Full=HST1;
GN   Name=STD1; Synonyms=STD;
OS   Cavia porcellus (Guinea pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC   Cavia.
OX   NCBI_TaxID=10141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=NIH 2; TISSUE=Adrenal gland;
RX   PubMed=8195238; DOI=10.1016/s0021-9258(17)40757-5;
RA   Lee Y.C., Park C.-S., Strott C.A.;
RT   "Molecular cloning of a chiral-specific 3 alpha-hydroxysteroid
RT   sulfotransferase.";
RL   J. Biol. Chem. 269:15838-15845(1994).
CC   -!- FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate
CC       (PAPS) as sulfonate donor to catalyze the sulfonation of 3-alpha-
CC       hydroxyl groups of neutral steroids.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-
CC         bisphosphate + an alkyl sulfate + H(+); Xref=Rhea:RHEA:22552,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:58339,
CC         ChEBI:CHEBI:58343, ChEBI:CHEBI:83414; EC=2.8.2.2;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Adrenal gland and liver.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 1 family. {ECO:0000305}.
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DR   EMBL; U06871; AAA19588.1; -; mRNA.
DR   PIR; A54026; A54026.
DR   RefSeq; NP_001166364.1; NM_001172893.1.
DR   AlphaFoldDB; P50234; -.
DR   SMR; P50234; -.
DR   STRING; 10141.ENSCPOP00000002750; -.
DR   GeneID; 100379581; -.
DR   KEGG; cpoc:100379581; -.
DR   CTD; 100379581; -.
DR   eggNOG; KOG1584; Eukaryota.
DR   InParanoid; P50234; -.
DR   Proteomes; UP000005447; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004027; F:alcohol sulfotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000863; Sulfotransferase_dom.
DR   Pfam; PF00685; Sulfotransfer_1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lipid metabolism; Reference proteome; Steroid metabolism;
KW   Transferase.
FT   CHAIN           1..287
FT                   /note="3-alpha-hydroxysteroid sulfotransferase"
FT                   /id="PRO_0000085139"
FT   ACT_SITE        99
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         44..49
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         72
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         77
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         129
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         184
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         218..223
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         247..249
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  33640 MW;  C95AA281C67F9581 CRC64;
     MSDNTPWFEG IRFPMVCFSP EILREVRDKF LVKDEDTITV TYPKSGTNWL NEIVCLILSK
     GDPKLVQSVP NWDRSPWIEF TGGYELVKGQ KDPRVYTSHL PLHLFPKSFF SSKAKVIYCI
     RNPRDALVSG YFFLSKMNVT EKPETLQQYM EWFLQGNVIY GSWFEHVRGW LSMREMENVL
     VLSYEDLIKD TRSTVEKICQ FLGKKLKPEE TDLVLKYSSF QFMKENEMSN FTLLPHAYTT
     EGFTLLRKGT VGDWKNHFTV AQAEAFDKIY QEKMAGYPPK LFSWEEC