SUL1_YEAST
ID SUL1_YEAST Reviewed; 859 AA.
AC P38359; D6VQT8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Sulfate permease 1;
DE AltName: Full=High-affinity sulfate transporter 1;
GN Name=SUL1; Synonyms=SFP2; OrderedLocusNames=YBR294W; ORFNames=YBR2110;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=INVSC1;
RX PubMed=7616962; DOI=10.1007/bf00290402;
RA Smith F.W., Hawkesford M.J., Prosser I.M., Clarkson D.T.;
RT "Isolation of a cDNA from Saccharomyces cerevisiae that encodes a high
RT affinity sulphate transporter at the plasma membrane.";
RL Mol. Gen. Genet. 247:709-715(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-585.
RC STRAIN=ATCC 44774 / DBY747, and LP1721-1B;
RX PubMed=7677785; DOI=10.1006/bbrc.1995.2343;
RA Jin Y.H., Jang Y.K., Kim M.J., Rad M.R., Kirchrath L., Seong R.H.,
RA Hong S.H., Hollenberg C.P., Park S.D.;
RT "Characterization of SFP2, a putative sulfate permease gene of
RT Saccharomyces cerevisiae.";
RL Biochem. Biophys. Res. Commun. 214:709-715(1995).
RN [6]
RP CHARACTERIZATION.
RX PubMed=9055073; DOI=10.1093/genetics/145.3.627;
RA Cherest H., Davidian J.C., Thomas D., Benes V., Ansorge W.,
RA Surdin-Kerjan Y.;
RT "Molecular characterization of two high affinity sulfate transporters in
RT Saccharomyces cerevisiae.";
RL Genetics 145:627-635(1997).
CC -!- FUNCTION: High affinity uptake of sulfate into the cell.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA84506.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Z36163; CAA85259.1; -; Genomic_DNA.
DR EMBL; X82013; CAA57540.1; -; mRNA.
DR EMBL; AY693178; AAT93197.1; -; Genomic_DNA.
DR EMBL; Z35134; CAA84506.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006936; DAA07408.1; -; Genomic_DNA.
DR PIR; S46176; S46176.
DR RefSeq; NP_009853.3; NM_001178642.3.
DR AlphaFoldDB; P38359; -.
DR SMR; P38359; -.
DR BioGRID; 32987; 55.
DR STRING; 4932.YBR294W; -.
DR TCDB; 2.A.53.1.1; the sulfate permease (sulp) family.
DR PaxDb; P38359; -.
DR PRIDE; P38359; -.
DR EnsemblFungi; YBR294W_mRNA; YBR294W; YBR294W.
DR GeneID; 852597; -.
DR KEGG; sce:YBR294W; -.
DR SGD; S000000498; SUL1.
DR VEuPathDB; FungiDB:YBR294W; -.
DR eggNOG; KOG0236; Eukaryota.
DR GeneTree; ENSGT01050000244925; -.
DR HOGENOM; CLU_003182_8_1_1; -.
DR InParanoid; P38359; -.
DR OMA; EWLPRYS; -.
DR BioCyc; YEAST:G3O-29212-MON; -.
DR Reactome; R-SCE-427601; Multifunctional anion exchangers.
DR PRO; PR:P38359; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38359; protein.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IMP:SGD.
DR GO; GO:0015301; F:anion:anion antiporter activity; IBA:GO_Central.
DR GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IMP:SGD.
DR GO; GO:0008272; P:sulfate transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR018045; S04_transporter_CS.
DR InterPro; IPR011547; SLC26A/SulP_dom.
DR InterPro; IPR001902; SLC26A/SulP_fam.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR PANTHER; PTHR11814; PTHR11814; 1.
DR Pfam; PF01740; STAS; 1.
DR Pfam; PF00916; Sulfate_transp; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR TIGRFAMs; TIGR00815; sulP; 1.
DR PROSITE; PS01130; SLC26A; 1.
DR PROSITE; PS50801; STAS; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..859
FT /note="Sulfate permease 1"
FT /id="PRO_0000080184"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 630..808
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 358
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 630
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 653
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 718
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 87
FT /note="D -> H (in Ref. 5; CAA84506)"
FT /evidence="ECO:0000305"
FT CONFLICT 457
FT /note="A -> T (in Ref. 5; CAA84506)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 859 AA; 95951 MW; A026DF572AF1FB53 CRC64;
MSRKSSTEYV HNQEDADIEV FESEYRTYRE SEAAENRDGL HNGDEENWKV NSSKQKFGVT
KNELSDVLYD SIPAYEESTV TLKEYYDHSI KNNLTAKSAG SYLVSLFPII KWFPHYNFTW
GYADLVAGIT VGCVLVPQSM SYAQIASLSP EYGLYSSFIG AFIYSLFATS KDVCIGPVAV
MSLQTAKVIA EVLKKYPEDQ TEVTAPIIAT TLCLLCGIVA TGLGILRLGF LVELISLNAV
AGFMTGSAFN IIWGQIPALM GYNSLVNTRE ATYKVVINTL KHLPNTKLDA VFGLIPLVIL
YVWKWWCGTF GITLADRYYR NQPKVANRLK SFYFYAQAMR NAVVIVVFTA ISWSITRNKS
SKDRPISILG TVPSGLNEVG VMKIPDGLLS NMSSEIPASI IVLVLEHIAI SKSFGRINDY
KVVPDQELIA IGVTNLIGTF FHSYPATGSF SRSALKAKCN VRTPFSGVFT GGCVLLALYC
LTDAFFFIPK ATLSAVIIHA VSDLLTSYKT TWTFWKTNPL DCISFIVTVF ITVFSSIENG
IYFAMCWSCA MLLLKQAFPA GKFLGRVEVA EVLNPTVQED IDAVISSNEL PNELNKQVKS
TVEVLPAPEY KFSVKWVPFD HGYSRELNIN TTVRPPPPGV IVYRLGDSFT YVNCSRHYDI
IFDRIKEETR RGQLITLRKK SDRPWNDPGE WKMPDSLKSL FKFKRHSATT NSDLPISNGS
SNGETYEKPL LKVVCLDFSQ VAQVDSTAVQ SLVDLRKAVN RYADRQVEFH FAGIISPWIK
RSLLSVKFGT TNEEYSDDSI IAGHSSFHVA KVLKDDVDYT DEDSRISTSY SNYETLCAAT
GTNLPFFHID IPDFSKWDV
