SUL2_YEAST
ID SUL2_YEAST Reviewed; 893 AA.
AC Q12325; D6VY92;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Sulfate permease 2;
DE AltName: Full=High-affinity sulfate transporter 2;
GN Name=SUL2; Synonyms=SEL2; OrderedLocusNames=YLR092W; ORFNames=L9449.1;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION.
RX PubMed=9055073; DOI=10.1093/genetics/145.3.627;
RA Cherest H., Davidian J.C., Thomas D., Benes V., Ansorge W.,
RA Surdin-Kerjan Y.;
RT "Molecular characterization of two high affinity sulfate transporters in
RT Saccharomyces cerevisiae.";
RL Genetics 145:627-635(1997).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: High affinity uptake of sulfate into the cell.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
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DR EMBL; U53880; AAB67596.1; -; Genomic_DNA.
DR EMBL; Z73264; CAA97653.1; -; Genomic_DNA.
DR EMBL; Z73265; CAA97655.1; -; Genomic_DNA.
DR EMBL; U53876; AAB67550.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09408.1; -; Genomic_DNA.
DR PIR; S64926; S64926.
DR RefSeq; NP_013193.1; NM_001181979.1.
DR AlphaFoldDB; Q12325; -.
DR SMR; Q12325; -.
DR BioGRID; 31365; 81.
DR DIP; DIP-5082N; -.
DR IntAct; Q12325; 4.
DR STRING; 4932.YLR092W; -.
DR TCDB; 2.A.53.1.13; the sulfate permease (sulp) family.
DR PaxDb; Q12325; -.
DR PRIDE; Q12325; -.
DR TopDownProteomics; Q12325; -.
DR EnsemblFungi; YLR092W_mRNA; YLR092W; YLR092W.
DR GeneID; 850781; -.
DR KEGG; sce:YLR092W; -.
DR SGD; S000004082; SUL2.
DR VEuPathDB; FungiDB:YLR092W; -.
DR eggNOG; KOG0236; Eukaryota.
DR GeneTree; ENSGT01050000244925; -.
DR HOGENOM; CLU_003182_8_1_1; -.
DR InParanoid; Q12325; -.
DR OMA; EMFARNH; -.
DR BioCyc; YEAST:G3O-32242-MON; -.
DR PRO; PR:Q12325; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12325; protein.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IMP:SGD.
DR GO; GO:0015301; F:anion:anion antiporter activity; IBA:GO_Central.
DR GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IMP:SGD.
DR GO; GO:0008272; P:sulfate transport; IMP:SGD.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR018045; S04_transporter_CS.
DR InterPro; IPR011547; SLC26A/SulP_dom.
DR InterPro; IPR001902; SLC26A/SulP_fam.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR PANTHER; PTHR11814; PTHR11814; 1.
DR Pfam; PF00916; Sulfate_transp; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR TIGRFAMs; TIGR00815; sulP; 1.
DR PROSITE; PS01130; SLC26A; 1.
DR PROSITE; PS50801; STAS; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..893
FT /note="Sulfate permease 2"
FT /id="PRO_0000080185"
FT TOPO_DOM 1..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..221
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..305
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..350
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..399
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 421..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..483
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 505..532
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 552..559
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 560..580
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 581..893
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 676..854
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 893 AA; 99651 MW; 67826955AC7C0BF5 CRC64;
MSREGYPNFE EVEIPDFQET NNTVPDLDDL ELEYDQYKNN ENNDTFNDKD LESNSVAKHN
AVNSSKGVKG SKIDYFNPSD VSLYDNSVSQ FEETTVSLKE YYDHSIRSHL TVKGACSYLK
SVFPIINWLP HYNFSWFTAD LIAGITIGCV LVPQSMSYAQ VATLPAQYGL YSSFIGAYSY
SFFATSKDVC IGPVAVMSLQ TAKVIADVTA KYPDGDSAIT GPVIATTLAL LCGIISAAVG
FLRLGFLVEL ISLNAVAGFM TGSAFNILWG QVPALMGYNS LVNTRAATYK VVIETLKHLP
DTKLDAVFGL IPLFLLYVWK WWCGTYGPRL NDRYNSKNPR LHKIIKWTYF YAQASRNGII
IIVFTCIGWA ITRGKSKSER PISILGSVPS GLKEVGVFHV PPGLMSKLGP NLPASIIVLL
LEHIAISKSF GRINDYKVVP DQELIAIGVS NLLGTFFNAY PATGSFSRSA LKAKCNVRTP
LSGLFSGSCV LLALYCLTGA FFYIPKATLS AVIIHAVSDL LASYQTTWNF WKMNPLDFIC
FIVTVLITVF ASIEDGIYFA MCWSCAMLIL KVAFPAGKFL GRVEVAEVTD AYVRPDSDVV
SYVSENNNGI STLEDGGEDD KESSTKYVTN SSKKIETNVQ TKGFDSPSSS ISQPRIKYHT
KWIPFDHKYT RELNPDVQIL PPPDGVLVYR LSESYTYLNC SRHYNIITEE VKKVTRRGQL
IRHRKKSDRP WNDPGPWEAP AFLKNLKFWK KRENDPESME NAPSTSVDVE RDDRPLLKIL
CLDFSQVAQT DATALQSLVD LRKAINQYAD RQVEFHFVGI ISPWVKRGLI SRGFGTLNEE
YSDESIVAGH TSYHVARVPQ GEENPEKYSV YTASGTNLPF FHIDIPDFAK WDI
