SULP_BACSU
ID SULP_BACSU Reviewed; 354 AA.
AC O34734; Q796I6;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Sulfate permease CysP;
GN Name=cysP; Synonyms=ylnA; OrderedLocusNames=BSU15580;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Foulger D., Errington J.;
RT "Cloning and sequencing 8 Kbp of DNA from Bacillus subtilis downstream of
RT the pyr operon.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INDUCTION.
RC STRAIN=168 / JH642;
RX PubMed=11004190; DOI=10.1128/jb.182.20.5885-5892.2000;
RA Mansilla M.C., Albanesi D., de Mendoza D.;
RT "Transcriptional control of the sulfur-regulated cysH operon, containing
RT genes involved in L-cysteine biosynthesis in Bacillus subtilis.";
RL J. Bacteriol. 182:5885-5892(2000).
RN [4]
RP FUNCTION AS A SULFATE PERMEASE, AND SUBCELLULAR LOCATION.
RC STRAIN=168 / JH642;
RX PubMed=10784039; DOI=10.1099/00221287-146-4-815;
RA Mansilla M.C., de Mendoza D.;
RT "The Bacillus subtilis cysP gene encodes a novel sulphate permease related
RT to the inorganic phosphate transporter (Pit) family.";
RL Microbiology 146:815-821(2000).
CC -!- FUNCTION: Involved in the import of sulfate.
CC {ECO:0000269|PubMed:10784039}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10784039};
CC Multi-pass membrane protein {ECO:0000269|PubMed:10784039}.
CC -!- INDUCTION: Up-regulated by sulfur starvation and repressed by cysteine.
CC Also induced by O-acetyl-L-serine (OAS), a direct precursor of
CC cysteine, maybe via inactivation of a putative transcriptional
CC repressor of the cysH operon whose activity is controlled by the
CC intracellular levels of OAS. {ECO:0000269|PubMed:11004190}.
CC -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC 2.A.20) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ000974; CAA04410.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13432.1; -; Genomic_DNA.
DR PIR; A69877; A69877.
DR RefSeq; NP_389441.1; NC_000964.3.
DR RefSeq; WP_003232101.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O34734; -.
DR SMR; O34734; -.
DR STRING; 224308.BSU15580; -.
DR TCDB; 2.A.20.4.1; the inorganic phosphate transporter (pit) family.
DR PaxDb; O34734; -.
DR EnsemblBacteria; CAB13432; CAB13432; BSU_15580.
DR GeneID; 935990; -.
DR KEGG; bsu:BSU15580; -.
DR PATRIC; fig|224308.179.peg.1698; -.
DR eggNOG; COG0306; Bacteria.
DR InParanoid; O34734; -.
DR OMA; RMFVAWG; -.
DR BioCyc; BSUB:BSU15580-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006817; P:phosphate ion transport; IEA:InterPro.
DR GO; GO:0008272; P:sulfate transport; IEA:UniProtKB-KW.
DR InterPro; IPR001204; Phos_transporter.
DR PANTHER; PTHR11101; PTHR11101; 1.
DR Pfam; PF01384; PHO4; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sulfate transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..354
FT /note="Sulfate permease CysP"
FT /id="PRO_0000378203"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 354 AA; 36836 MW; 800F20F686D72C38 CRC64;
MELAAILFSL FFAMNIGASG AAASMGVAYG SGAIKKKTYA LILCAVGVFA GAVIGGGEVV
KTISSGIIPE QTITLTIVCI IIGAAALSLF TANLLGIPLS TSEVTVGAVV GVGVAYKVLF
VNNLLIIVSF WVFVPLFAFG FTYFVSKLFR YFKIEVKSSK KQKILGIVLL VAGFFEAFSA
GMNNVANAVG PLVAAGVLDV GKGTLYGGAF VALGALLLGR RVLETNGKKI TRFSKGEGIL
LSGTGAGLVI ISSVFGMPVP LAQVTSSSII GIGMAKNGPN VFHKQVVQTM LKVWIVSPFL
SLSISYLLVS LFLKADYYSI FIMVSVLLAA GGAISLTKAI RKERRSVHEQ GGGI
