SUMM2_ARATH
ID SUMM2_ARATH Reviewed; 894 AA.
AC P60838; Q9LNB5;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Disease resistance protein SUMM2 {ECO:0000305};
DE AltName: Full=Disease resistance protein At1g12280 {ECO:0000305};
DE AltName: Full=Protein SUPPRESSOR OF MKK1 MKK2 2 {ECO:0000303|PubMed:22423965};
GN Name=SUMM2 {ECO:0000303|PubMed:22423965};
GN OrderedLocusNames=At1g12280 {ECO:0000312|Araport:AT1G12280};
GN ORFNames=F5O11.3 {ECO:0000312|EMBL:AAF79659.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, ACTIVITY REGULATION, AND MUTAGENESIS OF GLY-2; SER-33; PRO-152;
RP GLY-186; LEU-255; PRO-307 AND ASP-478.
RX PubMed=22423965; DOI=10.1016/j.chom.2012.01.015;
RA Zhang Z., Wu Y., Gao M., Zhang J., Kong Q., Liu Y., Ba H., Zhou J.,
RA Zhang Y.;
RT "Disruption of PAMP-induced MAP kinase cascade by a Pseudomonas syringae
RT effector activates plant immunity mediated by the NB-LRR protein SUMM2.";
RL Cell Host Microbe 11:253-263(2012).
RN [4]
RP FUNCTION.
RX PubMed=22643122; DOI=10.1105/tpc.112.097253;
RA Kong Q., Qu N., Gao M., Zhang Z., Ding X., Yang F., Li Y., Dong O.X.,
RA Chen S., Li X., Zhang Y.;
RT "The MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates immunity
RT mediated by a mitogen-activated protein kinase kinase kinase in
RT Arabidopsis.";
RL Plant Cell 24:2225-2236(2012).
RN [5]
RP INTERACTION WITH PAT1.
RX PubMed=25603932; DOI=10.15252/embj.201488645;
RA Roux M.E., Rasmussen M.W., Palma K., Lolle S., Regue A.M., Bethke G.,
RA Glazebrook J., Zhang W., Sieburth L., Larsen M.R., Mundy J., Petersen M.;
RT "The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4
RT and immune receptor SUMM2.";
RL EMBO J. 34:593-608(2015).
CC -!- FUNCTION: Disease resistance protein that mediates defense responses
CC against the bacterial pathogen Pseudomonas syringae pv tomato strain
CC DC3000, and the virulent oomycete Hyaloperonospora arabidopsidis
CC isolate Noco2. Becomes active when the MEKK1-MKK1-MKK2-MPK4 kinase
CC cascade is disrupted by the microbial effector hopAI1. Does not seem to
CC be required for the activation of MPK4 by flg22, or flg22-induced up-
CC regulation of PAD3 (PubMed:22423965). Functions downstream of
CC MEKK2/SUMM1 in immune responses, including cell death and defense
CC responses (PubMed:22643122). {ECO:0000269|PubMed:22423965,
CC ECO:0000269|PubMed:22643122}.
CC -!- ACTIVITY REGULATION: Negatively regulated by the MEKK1-MKK1-MKK2-MPK4
CC kinase cascade. {ECO:0000269|PubMed:22423965}.
CC -!- SUBUNIT: Interacts with PAT1. {ECO:0000269|PubMed:25603932}.
CC -!- DOMAIN: The LRR repeats probably act as specificity determinant of
CC pathogen recognition. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79659.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AC025416; AAF79659.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28861.1; -; Genomic_DNA.
DR RefSeq; NP_172692.1; NM_101100.2.
DR AlphaFoldDB; P60838; -.
DR SMR; P60838; -.
DR BioGRID; 23022; 3.
DR STRING; 3702.AT1G12280.1; -.
DR iPTMnet; P60838; -.
DR PaxDb; P60838; -.
DR PRIDE; P60838; -.
DR ProteomicsDB; 228402; -.
DR EnsemblPlants; AT1G12280.1; AT1G12280.1; AT1G12280.
DR GeneID; 837782; -.
DR Gramene; AT1G12280.1; AT1G12280.1; AT1G12280.
DR KEGG; ath:AT1G12280; -.
DR Araport; AT1G12280; -.
DR TAIR; locus:2034770; AT1G12280.
DR eggNOG; KOG4658; Eukaryota.
DR HOGENOM; CLU_000427_4_0_1; -.
DR InParanoid; P60838; -.
DR OMA; DMAMWIA; -.
DR OrthoDB; 236715at2759; -.
DR PhylomeDB; P60838; -.
DR PRO; PR:P60838; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P60838; baseline and differential.
DR Genevisible; P60838; AT.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IGI:TAIR.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Leucine-rich repeat; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..894
FT /note="Disease resistance protein SUMM2"
FT /id="PRO_0000212733"
FT DOMAIN 140..443
FT /note="NB-ARC"
FT REPEAT 517..538
FT /note="LRR 1"
FT REPEAT 539..561
FT /note="LRR 2"
FT REPEAT 564..586
FT /note="LRR 3"
FT REPEAT 588..610
FT /note="LRR 4"
FT REPEAT 611..633
FT /note="LRR 5"
FT REPEAT 634..656
FT /note="LRR 6"
FT REPEAT 660..681
FT /note="LRR 7"
FT COILED 31..71
FT /evidence="ECO:0000255"
FT BINDING 183..190
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 2
FT /note="G->R: In summ2-5; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 33
FT /note="S->F: In summ2-6; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 152
FT /note="P->S: In summ2-7; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 186
FT /note="G->E: In summ2-2; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 255
FT /note="L->S: In summ2-1; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 307
FT /note="P->S: In summ2-3; suppresses the extreme dwarfism
FT and autoimune phenotype of the double mutant mkk1 and
FT mkk2."
FT /evidence="ECO:0000269|PubMed:22423965"
FT MUTAGEN 478
FT /note="D->V: Constitutively active protein that induces
FT pathogen-independent hypersensitive response."
FT /evidence="ECO:0000269|PubMed:22423965"
SQ SEQUENCE 894 AA; 102641 MW; DBA6F5CF9A48BB96 CRC64;
MGACLTLSFS CDEVVNQISQ GLCINVGYIC ELSKNVVAMK KDMEVLKKKR DDVKRRVDIE
EFTRRRERLS QVQGWLTNVS TVENKFNELL TTNDAELQRL CLFGFCSKNV KMSYLYGKRV
VLMLKEIESL SSQGDFDTVT LATPIARIEE MPIQPTIVGQ ETMLERVWTR LTEDGDEIVG
LYGMGGVGKT TLLTRINNKF SEKCSGFGVV IWVVVSKSPD IHRIQGDIGK RLDLGGEEWD
NVNENQRALD IYNVLGKQKF VLLLDDIWEK VNLEVLGVPY PSRQNGCKVV FTTRSRDVCG
RMRVDDPMEV SCLEPNEAWE LFQMKVGENT LKGHPDIPEL ARKVAGKCCG LPLALNVIGE
TMACKRMVQE WRNAIDVLSS YAAEFPGMEQ ILPILKYSYD NLNKEQVKPC FLYCSLFPED
YRMEKERLID YWICEGFIDE NESRERALSQ GYEIIGILVR ACLLLEEAIN KEQVKMHDVV
REMALWIASD LGEHKERCIV QVGVGLREVP KVKNWSSVRR MSLMENEIEI LSGSPECLEL
TTLFLQKNDS LLHISDEFFR CIPMLVVLDL SGNSSLRKLP NQISKLVSLR YLDLSWTYIK
RLPVGLQELK KLRYLRLDYM KRLKSISGIS NISSLRKLQL LQSKMSLDMS LVEELQLLEH
LEVLNISIKS SLVVEKLLNA PRLVKCLQIL VLRGVQEESS GVLTLPDMDN LNKVIIRKCG
MCEIKIERKT LSLSSNRSPK TQFLHNLSTV HISSCDGLKD LTWLLFAPNL TSLEVLDSEL
VEGIINQEKA MTMSGIIPFQ KLESLRLHNL AMLRSIYWQP LSFPCLKTIH ITKCPELRKL
PLDSEIAIRD EELVIKYQEE EWLERVEWDN EATRLRFLPF FKFFGPEWQV SYVR
