SUN1_ASPFU
ID SUN1_ASPFU Reviewed; 414 AA.
AC Q4WGL5;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Secreted beta-glucosidase sun1;
DE EC=3.2.1.-;
DE Flags: Precursor;
GN Name=sun1; ORFNames=AFUA_7G05450;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP INDUCTION, DISRUPTION PHENOTYPE, FUNCTION, GLYCOSYLATION, CATALYTIC
RP ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=23508952; DOI=10.1074/jbc.m112.440172;
RA Gastebois A., Aimanianda V., Bachellier-Bassi S., Nesseir A., Firon A.,
RA Beauvais A., Schmitt C., England P., Beau R., Prevost M.C., d'Enfert C.,
RA Latge J.P., Mouyna I.;
RT "SUN proteins belong to a novel family of beta-(1,3)-glucan-modifying
RT enzymes involved in fungal morphogenesis.";
RL J. Biol. Chem. 288:13387-13396(2013).
CC -!- FUNCTION: Cell surface beta-glucosidase involved in cell wall
CC biosynthesis and septation, and thus required for normal growth and
CC correct hyphal morphogenesis. Has hydrolytic activity on linear (1->3)-
CC beta-D-glucans such as laminaribiose and other
CC laminarioligosaccharides. Has also a minor transferase activity.
CC {ECO:0000269|PubMed:23508952}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 5.5. {ECO:0000269|PubMed:23508952};
CC Temperature dependence:
CC Optimum temperature is 37 degrees Celsius.
CC {ECO:0000269|PubMed:23508952};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Secreted.
CC -!- INDUCTION: Expression was detected 4h after the initiation of spore
CC germination and during mycelial growth. {ECO:0000269|PubMed:23508952}.
CC -!- PTM: Highly glycosylated. {ECO:0000269|PubMed:23508952}.
CC -!- DISRUPTION PHENOTYPE: Leads to smaller colonies and swollen and
CC vacuolated hyphae with double cell wall and leaky tips. Leads also to
CC decreased conidiation. {ECO:0000269|PubMed:23508952}.
CC -!- SIMILARITY: Belongs to the SUN family. {ECO:0000305}.
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DR EMBL; AAHF01000009; EAL86926.1; -; Genomic_DNA.
DR RefSeq; XP_748964.1; XM_743871.1.
DR AlphaFoldDB; Q4WGL5; -.
DR SMR; Q4WGL5; -.
DR STRING; 746128.CADAFUBP00008850; -.
DR CAZy; GH132; Glycoside Hydrolase Family 132.
DR CLAE; EXG132A_ASPFU; -.
DR EnsemblFungi; EAL86926; EAL86926; AFUA_7G05450.
DR GeneID; 3506240; -.
DR KEGG; afm:AFUA_7G05450; -.
DR VEuPathDB; FungiDB:Afu7g05450; -.
DR eggNOG; ENOG502QPVV; Eukaryota.
DR HOGENOM; CLU_033459_1_0_1; -.
DR InParanoid; Q4WGL5; -.
DR OMA; CSYACQS; -.
DR OrthoDB; 1130819at2759; -.
DR Proteomes; UP000002530; Chromosome 7.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IDA:AspGD.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0006076; P:(1->3)-beta-D-glucan catabolic process; IDA:AspGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR GO; GO:0030448; P:hyphal growth; IMP:AspGD.
DR InterPro; IPR005556; SUN.
DR Pfam; PF03856; SUN; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cell wall; Cell wall biogenesis/degradation;
KW Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..414
FT /note="Secreted beta-glucosidase sun1"
FT /id="PRO_0000425080"
FT REGION 115..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 377
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 414 AA; 43504 MW; D99C641D515F8824 CRC64;
MKFNTVALTL ATAGSLVTAQ HHHQHRHHQH KREDVVESSA TVVQYELDGK PISLKQVCAG
LADNTLKFAN NDHPTGICDN LSSAAAPAST PEVTSAFAPA QFIELSSVVT SATPTSASSS
ETVQTPAASS SSASSSSTAT GLDADFPDGE LDCSTFPSEY GAIPLDYLKL GGWSGIQYVS
YAGNFINDIV TAVAGDTCKD GAMCSYACPP GYQKSQWPST QGATGQSVGG IECRNGKLHL
TNPSLSKKLC IPGVGGVHVQ NTLGETVAVC RTDYPGTESE TIPIGLGGND LQPLTCPDGE
TYYKWQGKTT SAQYYVNPKG VTPEKGCQWG DGTQPIGNWA PVNLGVGLNK GKWLSIFQNS
PTTSEKLDFN IKIKGDNLSG SCKYENGVFY SETGSSSSGC TVQVMSGDAT FVFY
