SUN41_CANAL
ID SUN41_CANAL Reviewed; 418 AA.
AC Q59NP5; A0A1D8PPG8;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Secreted beta-glucosidase SUN41;
DE EC=3.2.1.-;
DE Flags: Precursor;
GN Name=SUN41 {ECO:0000303|PubMed:12492856}; Synonyms=SUN4;
GN OrderedLocusNames=CAALFM_C600820WA; ORFNames=CaO19.11124, CaO19.3642;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PREDICTION AS A SUBSTRATE FOR KEX2 CLEAVAGE.
RX PubMed=12419804; DOI=10.1074/jbc.m209713200;
RA Newport G., Kuo A., Flattery A., Gill C., Blake J.J., Kurtz M.B.,
RA Abruzzo G.K., Agabian N.;
RT "Inactivation of Kex2p diminishes the virulence of Candida albicans.";
RL J. Biol. Chem. 278:1713-1720(2003).
RN [5]
RP INDUCTION.
RX PubMed=12492856; DOI=10.1046/j.1365-2958.2003.03300.x;
RA Sohn K., Urban C., Brunner H., Rupp S.;
RT "EFG1 is a major regulator of cell wall dynamics in Candida albicans as
RT revealed by DNA microarrays.";
RL Mol. Microbiol. 47:89-102(2003).
RN [6]
RP INDUCTION.
RX PubMed=15917516; DOI=10.1128/aac.49.6.2226-2236.2005;
RA Liu T.T., Lee R.E., Barker K.S., Lee R.E., Wei L., Homayouni R.,
RA Rogers P.D.;
RT "Genome-wide expression profiling of the response to azole, polyene,
RT echinocandin, and pyrimidine antifungal agents in Candida albicans.";
RL Antimicrob. Agents Chemother. 49:2226-2236(2005).
RN [7]
RP INDUCTION.
RX PubMed=16854431; DOI=10.1016/j.jmb.2006.06.040;
RA Setiadi E.R., Doedt T., Cottier F., Noffz C., Ernst J.F.;
RT "Transcriptional response of Candida albicans to hypoxia: linkage of oxygen
RT sensing and Efg1p-regulatory networks.";
RL J. Mol. Biol. 361:399-411(2006).
RN [8]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=17873081; DOI=10.1128/ec.00314-07;
RA Norice C.T., Smith F.J. Jr., Solis N., Filler S.G., Mitchell A.P.;
RT "Requirement for Candida albicans Sun41 in biofilm formation and
RT virulence.";
RL Eukaryot. Cell 6:2046-2055(2007).
RN [9]
RP DISRUPTION PHENOTYPE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP SUBCELLULAR LOCATION.
RX PubMed=17905924; DOI=10.1128/ec.00285-07;
RA Hiller E., Heine S., Brunner H., Rupp S.;
RT "Candida albicans Sun41p, a putative glycosidase, is involved in
RT morphogenesis, cell wall biogenesis, and biofilm formation.";
RL Eukaryot. Cell 6:2056-2065(2007).
RN [10]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=18001349; DOI=10.1111/j.1365-2958.2007.06011.x;
RA Firon A., Aubert S., Iraqui I., Guadagnini S., Goyard S., Prevost M.C.,
RA Janbon G., d'Enfert C.;
RT "The SUN41 and SUN42 genes are essential for cell separation in Candida
RT albicans.";
RL Mol. Microbiol. 66:1256-1275(2007).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=19819358; DOI=10.1016/j.jprot.2009.10.003;
RA Thomas D.P., Lopez-Ribot J.L., Lee S.A.;
RT "A proteomic analysis of secretory proteins of a pre-vacuolar mutant of
RT Candida albicans.";
RL J. Proteomics 73:342-351(2009).
RN [12]
RP INDUCTION.
RX PubMed=19798425; DOI=10.1371/journal.ppat.1000601;
RA Sahni N., Yi S., Daniels K.J., Srikantha T., Pujol C., Soll D.R.;
RT "Genes selectively up-regulated by pheromone in white cells are involved in
RT biofilm formation in Candida albicans.";
RL PLoS Pathog. 5:E1000601-E1000601(2009).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=21622905; DOI=10.1128/ec.05011-11;
RA Sorgo A.G., Heilmann C.J., Dekker H.L., Bekker M., Brul S., de Koster C.G.,
RA de Koning L.J., Klis F.M.;
RT "Effects of fluconazole on the secretome, the wall proteome, and wall
RT integrity of the clinical fungus Candida albicans.";
RL Eukaryot. Cell 10:1071-1081(2011).
RN [14]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=23508952; DOI=10.1074/jbc.m112.440172;
RA Gastebois A., Aimanianda V., Bachellier-Bassi S., Nesseir A., Firon A.,
RA Beauvais A., Schmitt C., England P., Beau R., Prevost M.C., d'Enfert C.,
RA Latge J.P., Mouyna I.;
RT "SUN proteins belong to a novel family of beta-(1,3)-glucan-modifying
RT enzymes involved in fungal morphogenesis.";
RL J. Biol. Chem. 288:13387-13396(2013).
CC -!- FUNCTION: Cell surface beta-glucosidase involved in cytokinesis, cell
CC wall biogenesis, adhesion to host tissue, and biofilm formation; thus
CC playing an important role in the host-pathogen interaction. Has
CC hydrolytic activity on linear (1->3)-beta-D-glucans such as
CC laminaribiose and other laminarioligosaccharides.
CC {ECO:0000269|PubMed:17873081, ECO:0000269|PubMed:17905924,
CC ECO:0000269|PubMed:18001349, ECO:0000269|PubMed:23508952}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 5.5. {ECO:0000269|PubMed:23508952};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:21622905}. Secreted {ECO:0000269|PubMed:17905924,
CC ECO:0000269|PubMed:19819358}. Note=Secreted into the medium of cells
CC growing as blastospores as well as those forming hyphae
CC (PubMed:17905924). Secretion is decreased in cells lacking VPS4
CC (PubMed:19819358). {ECO:0000269|PubMed:17905924,
CC ECO:0000269|PubMed:19819358}.
CC -!- INDUCTION: Strongly up-regulated under hypoxic conditions and in white
CC but not opaque cells (PubMed:16854431, PubMed:19798425). Repressed by
CC exposure to caspofungin (PubMed:15917516). Expression is probably
CC regulated by EFG1 since SUN41 has 9 E-boxes in its promoter
CC (PubMed:12492856). {ECO:0000269|PubMed:12492856,
CC ECO:0000269|PubMed:15917516, ECO:0000269|PubMed:16854431,
CC ECO:0000269|PubMed:19798425}.
CC -!- PTM: Predicted to be a substrate for cleavage by KEX2.
CC {ECO:0000305|PubMed:12419804}.
CC -!- DISRUPTION PHENOTYPE: Leads to increased cell size, cytokinesis
CC defects, altered sensitivity to the cell wall-modifying substance Congo
CC red, defects in adhesion, and reduced biofilm formation.
CC {ECO:0000269|PubMed:17873081, ECO:0000269|PubMed:17905924,
CC ECO:0000269|PubMed:18001349}.
CC -!- SIMILARITY: Belongs to the SUN family. {ECO:0000305}.
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DR EMBL; CP017628; AOW30028.1; -; Genomic_DNA.
DR RefSeq; XP_711315.2; XM_706223.2.
DR AlphaFoldDB; Q59NP5; -.
DR BioGRID; 1230112; 3.
DR STRING; 237561.Q59NP5; -.
DR CAZy; GH132; Glycoside Hydrolase Family 132.
DR GeneID; 3647067; -.
DR KEGG; cal:CAALFM_C600820WA; -.
DR CGD; CAL0000175157; SUN41.
DR VEuPathDB; FungiDB:C6_00820W_A; -.
DR HOGENOM; CLU_033459_2_0_1; -.
DR InParanoid; Q59NP5; -.
DR OMA; CSYACQS; -.
DR OrthoDB; 1130819at2759; -.
DR PRO; PR:Q59NP5; -.
DR Proteomes; UP000000559; Chromosome 6.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR005556; SUN.
DR Pfam; PF03856; SUN; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cell wall; Cell wall biogenesis/degradation;
KW Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..418
FT /note="Secreted beta-glucosidase SUN41"
FT /id="PRO_0000425079"
FT REGION 81..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 418 AA; 43693 MW; 50A64905AD1E7B5A CRC64;
MRFSQATVLA FAALSLAAPA FEADNKNIKR EDCDKTSFHG HHKHKRAVAY DYAYVTVTVD
GNGNPITTVS PVLSIETIAK SEETSSTSTS ISSTTTIVQN DSLTSDEPKT LSLPSGTIKP
SSFATESQSQ SQSSSTGGSG SGSTNGIEGD LAAFEDPTEE FEDGVLSCSD FPSGQGVIPL
DHLGFGGWSG IENSDGSTGG NCKEGSYCSY ACQSGMSKTQ WPEDQPSNGV SIGGLLCKNG
KLYKSSTRSN YLCEWGVKKA NVVNKLSETV AICRTDYPGT ENMVIPTVVG GGSTSVITVV
DQSTYYTWRG GATSAQYYVN NAGVSWEDGC VWGTPGSGVG NWAPLNFGAG YANGIAYLSL
IPNPNNRDSL NFKVKIVGES GSTVSGSCSY ANGKFNGNSD DGCTVGVTSG EADFVLYN
