SUN4_ARATH
ID SUN4_ARATH Reviewed; 596 AA.
AC F4I8I0; Q0WQI7; Q9C9H3;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=SUN domain-containing protein 4 {ECO:0000305};
DE Short=AtSUN4 {ECO:0000303|PubMed:25217773};
GN Name=SUN4 {ECO:0000303|PubMed:25217773};
GN OrderedLocusNames=At1g71360 {ECO:0000312|Araport:AT1G71360};
GN ORFNames=F26A9.26 {ECO:0000312|EMBL:AAG51822.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH SUN1; SUN2; SUN3 AND TIK,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=25217773; DOI=10.1093/jxb/eru368;
RA Graumann K., Vanrobays E., Tutois S., Probst A.V., Evans D.E., Tatout C.;
RT "Characterization of two distinct subfamilies of SUN-domain proteins in
RT Arabidopsis and their interactions with the novel KASH-domain protein
RT AtTIK.";
RL J. Exp. Bot. 65:6499-6512(2014).
RN [5]
RP REVIEW.
RX PubMed=25740919; DOI=10.1093/jxb/erv082;
RA Zhou X., Graumann K., Meier I.;
RT "The plant nuclear envelope as a multifunctional platform LINCed by SUN and
RT KASH.";
RL J. Exp. Bot. 66:1649-1659(2015).
CC -!- FUNCTION: Encodes a member of the mid-SUN subfamily of SUN-domain
CC proteins that is localized to both the nuclear envelope and the ER. It
CC is involved in early seed development and nuclear morphology. [TAIR].
CC -!- SUBUNIT: Forms homomers and heteromers with SUN3. Interacts with SUN1,
CC SUN2 and TIK. {ECO:0000269|PubMed:25217773}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:25217773};
CC Multi-pass membrane protein {ECO:0000255}. Endoplasmic reticulum
CC membrane {ECO:0000269|PubMed:25217773}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4I8I0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I8I0-2; Sequence=VSP_059087;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Embryo lethal when
CC associated with disruption mutants SUN3 and SUN5.
CC {ECO:0000269|PubMed:25217773}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51822.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC016163; AAG51822.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35193.1; -; Genomic_DNA.
DR EMBL; AK228709; BAF00612.1; -; mRNA.
DR RefSeq; NP_177292.4; NM_105805.6. [F4I8I0-1]
DR AlphaFoldDB; F4I8I0; -.
DR SMR; F4I8I0; -.
DR STRING; 3702.AT1G71360.1; -.
DR iPTMnet; F4I8I0; -.
DR PaxDb; F4I8I0; -.
DR PRIDE; F4I8I0; -.
DR EnsemblPlants; AT1G71360.1; AT1G71360.1; AT1G71360. [F4I8I0-1]
DR GeneID; 843477; -.
DR Gramene; AT1G71360.1; AT1G71360.1; AT1G71360. [F4I8I0-1]
DR KEGG; ath:AT1G71360; -.
DR Araport; AT1G71360; -.
DR TAIR; locus:2825334; AT1G71360.
DR eggNOG; KOG1396; Eukaryota.
DR HOGENOM; CLU_022850_2_0_1; -.
DR InParanoid; F4I8I0; -.
DR OMA; DSWKSHI; -.
DR PRO; PR:F4I8I0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I8I0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043621; F:protein self-association; IPI:UniProtKB.
DR GO; GO:0006997; P:nucleus organization; IGI:TAIR.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR045120; Suco/Slp1-like.
DR InterPro; IPR012919; SUN_dom.
DR PANTHER; PTHR12953; PTHR12953; 1.
DR Pfam; PF07738; Sad1_UNC; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR PROSITE; PS51469; SUN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum; Membrane;
KW Nucleus; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..596
FT /note="SUN domain-containing protein 4"
FT /id="PRO_0000441679"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 545..565
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 576..596
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 179..343
FT /note="SUN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00802"
FT REGION 58..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 475..544
FT /evidence="ECO:0000255"
FT COMPBIAS 79..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 368..520
FT /note="Missing (in isoform 2)"
FT /id="VSP_059087"
SQ SEQUENCE 596 AA; 67272 MW; 9F8F796BFEE32E58 CRC64;
MQRSRRALLV RRRVSETTSN GRNRFYKVSL SLVFLIWGLV FLSTLWISHV DGDKGRSLVD
SVEKGEPDDE RADETAESVD ATSLESTSVH SNPGLSSDVD IAAAGESKGS ETILKQLEVD
NTIVIVGNVT ESKDNVPMKQ SEINNNTVPG NDTETTGSKL DQLSRAVPLG LDEFKSRASN
SRDKSLSGQV TGVIHRMEPG GKEYNYAAAS KGAKVLSSNK EAKGASSIIC RDKDKYLRNP
CSTEGKFVVI ELSEETLVNT IKIANFEHYS SNLKDFEILG TLVYPTDTWV HLGNFTALNM
KHEQNFTFAD PKWVRYLKLN LLSHYGSEFY CTLSLLEVYG VDAVERMLED LISIQDKNIL
KLQEGDTEQK EKKTMQAKES FESDEDKSKQ KEKEQEASPE NAVVKDEVSL EKRKLPDPVE
EIKHQPGSRM PGDTVLKILM QKIRSLDVSL SVLESYLEER SLKYGMIFKE MDLEASKREK
EVETMRLEVE GMKEREENTK KEAMEMRKWR MRVETELEKA ENEKEKVKER LEQVLERLEW
MEKKGVVVFT ICVGFGTIAV VAVVFGMGIV RAEKQGGLAW LLLLISSTFV MFILSL
