SUN4_YEAST
ID SUN4_YEAST Reviewed; 420 AA.
AC P53616; A2TBM4; D6W1B4; Q6B1F2; Q870H0;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Probable secreted beta-glucosidase SUN4;
DE EC=3.2.1.-;
DE AltName: Full=Septation protein SUN4;
DE AltName: Full=Soluble cell wall protein 3;
DE Flags: Precursor;
GN Name=SUN4; Synonyms=SCW3; OrderedLocusNames=YNL066W;
GN ORFNames=N2411, YNL2411W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / FY1676;
RX PubMed=8533472; DOI=10.1002/yea.320111008;
RA Bergez P., Doignon F., Crouzet M.;
RT "The sequence of a 44 420 bp fragment located on the left arm of chromosome
RT XIV from Saccharomyces cerevisiae.";
RL Yeast 11:967-974(1995).
RN [2]
RP ERRATUM OF PUBMED:8533472.
RX PubMed=8904343;
RX DOI=10.1002/(sici)1097-0061(19960315)12:3<297::aid-yea940>3.0.co;2-d;
RA Bergez P., Doignon F., Crouzet M.;
RL Yeast 12:297-297(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8701611;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<391::aid-yea921>3.0.co;2-n;
RA Poehlmann R., Philippsen P.;
RT "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT reveals 12 new open reading frames (ORFs) and an ancient duplication of six
RT ORFs.";
RL Yeast 12:391-402(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-92.
RC STRAIN=ATCC 204508 / S288c;
RA Zhang Z., Dietrich F.S.;
RT "YNL066W(SUN4) mRNA.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-66.
RC STRAIN=ATCC 201390 / BY4743;
RX PubMed=17244705; DOI=10.1073/pnas.0610354104;
RA Juneau K., Palm C., Miranda M., Davis R.W.;
RT "High-density yeast-tiling array reveals previously undiscovered introns
RT and extensive regulation of meiotic splicing.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007).
RN [9]
RP PROTEIN SEQUENCE OF 25-35, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 96099 / S288c / SEY6210;
RX PubMed=9748433; DOI=10.1128/jb.180.19.5030-5037.1998;
RA Cappellaro C., Mrsa V., Tanner W.;
RT "New potential cell wall glucanases of Saccharomyces cerevisiae and their
RT involvement in mating.";
RL J. Bacteriol. 180:5030-5037(1998).
RN [10]
RP FUNCTION.
RX PubMed=10870102;
RX DOI=10.1002/1097-0061(200007)16:10<905::aid-yea584>3.0.co;2-1;
RA Mouassite M., Camougrand N.M., Schwob E., Demaison G., Laclau M.,
RA Guerin M.G.;
RT "The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation.";
RL Yeast 16:905-919(2000).
RN [11]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [12]
RP SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=24040106; DOI=10.1371/journal.pone.0073882;
RA Kuznetsov E., Kucerova H., Vachova L., Palkova Z.;
RT "SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces
RT cerevisiae and produced dependently on oxygen level.";
RL PLoS ONE 8:E73882-E73882(2013).
CC -!- FUNCTION: Involved in the remodeling of the cell wall during the
CC various phases of yeast culture development and under various
CC environmental conditions and plays a role in septation.
CC {ECO:0000269|PubMed:10870102, ECO:0000269|PubMed:24040106}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:24040106,
CC ECO:0000269|PubMed:9748433}.
CC -!- INDUCTION: Expression is decreased during transition to slow growing or
CC stationary phases. {ECO:0000269|PubMed:24040106}.
CC -!- PTM: Glycosylated.
CC -!- DISRUPTION PHENOTYPE: Leads to increased resistance to zymolyase
CC treatment. {ECO:0000269|PubMed:24040106}.
CC -!- MISCELLANEOUS: Present with 24700 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SUN family. {ECO:0000305}.
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DR EMBL; U12141; AAA99645.1; -; Genomic_DNA.
DR EMBL; X86470; CAA60196.1; -; Genomic_DNA.
DR EMBL; Z71342; CAA95939.1; -; Genomic_DNA.
DR EMBL; AY693128; AAT93147.1; -; Genomic_DNA.
DR EMBL; AY245796; AAP04346.1; -; mRNA.
DR EMBL; EF123127; ABM97471.1; -; mRNA.
DR EMBL; BK006947; DAA10480.1; -; Genomic_DNA.
DR PIR; S53916; S53916.
DR RefSeq; NP_014333.1; NM_001182904.1.
DR AlphaFoldDB; P53616; -.
DR BioGRID; 35757; 125.
DR DIP; DIP-4365N; -.
DR STRING; 4932.YNL066W; -.
DR CAZy; GH132; Glycoside Hydrolase Family 132.
DR MaxQB; P53616; -.
DR PaxDb; P53616; -.
DR PRIDE; P53616; -.
DR EnsemblFungi; YNL066W_mRNA; YNL066W; YNL066W.
DR GeneID; 855659; -.
DR KEGG; sce:YNL066W; -.
DR SGD; S000005010; SUN4.
DR VEuPathDB; FungiDB:YNL066W; -.
DR eggNOG; ENOG502QPVV; Eukaryota.
DR GeneTree; ENSGT00940000176328; -.
DR HOGENOM; CLU_033459_2_0_1; -.
DR InParanoid; P53616; -.
DR OMA; NGECIYE; -.
DR BioCyc; YEAST:G3O-33096-MON; -.
DR PRO; PR:P53616; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53616; protein.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0015926; F:glucosidase activity; ISS:SGD.
DR GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR GO; GO:0007005; P:mitochondrion organization; TAS:SGD.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR005556; SUN.
DR Pfam; PF03856; SUN; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Cell cycle; Cell division; Cell wall;
KW Cell wall biogenesis/degradation; Direct protein sequencing; Glycoprotein;
KW Glycosidase; Hydrolase; Polysaccharide degradation; Reference proteome;
KW Secreted; Septation; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:9748433"
FT CHAIN 25..420
FT /note="Probable secreted beta-glucosidase SUN4"
FT /id="PRO_0000033466"
FT REGION 89..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 395
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 299
FT /note="L -> S (in Ref. 6; AAT93147)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 420 AA; 43442 MW; F1FB6CD46F2CDA13 CRC64;
MKLSATTLTA ASLIGYSTIV SALPYAADID TGCTTTAHGS HQHKRAVAVT YVYETVTVDK
NGQTVTPTST EASSTVASTT TLISESSVTK SSSKVASSSE STEQIATTSS SAQTTLTSSE
TSTSESSVPI STSGSASTSS AASSATGSIY GDLADFSGPY EKFEDGTIPC GQFPSGQGVI
PISWLDEGGW SGVENTDTST GGSCKEGSYC SYACQPGMSK TQWPSDQPSD GRSIGGLLCK
DGYLYRSNTD TDYLCEWGVD AAYVVSELSN DVAICRTDYP GTENMVIPTY VQAGDSLPLT
VVDQDTYYTW QGLKTSAQYY VNNAGISVED ACVWGSSSSG VGNWAPLNFG AGSSDGVAYL
SLIPNPNNGN ALNFNVKIVA ADDSSTVNGE CIYENGSFSG GSDGCTVSVT AGKAKFVLYN
