SUP_ARATH
ID SUP_ARATH Reviewed; 204 AA.
AC Q38895; Q4PSN1;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Transcriptional regulator SUPERMAN;
GN Name=SUP; Synonyms=FLO10; OrderedLocusNames=At3g23130; ORFNames=K14B15.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTANT SUP-3.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=7477325; DOI=10.1038/378199a0;
RA Sakai H., Medrano L.J., Meyerowitz E.M.;
RT "Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries.";
RL Nature 378:199-203(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND MUTANT FLO-10.
RC STRAIN=cv. Columbia;
RX PubMed=12324589; DOI=10.2307/3869229;
RA Schultz E.A., Pickett F.B., Haughn G.W.;
RT "The FLO10 gene product regulates the expression domain of homeotic genes
RT AP3 and PI in Arabidopsis flowers.";
RL Plant Cell 3:1221-1237(1991).
RN [6]
RP FUNCTION, AND MUTANT SUP-1.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=1352237; DOI=10.1242/dev.114.3.599;
RA Bowman J.L., Sakai H., Jack T., Weigel D., Mayer U., Meyerowitz E.M.;
RT "SUPERMAN, a regulator of floral homeotic genes in Arabidopsis.";
RL Development 114:599-615(1992).
RN [7]
RP FUNCTION, AND GENETIC REGULATION.
RX PubMed=11006335; DOI=10.2307/3871177;
RA Sakai H., Krizek B.A., Jacobsen S.E., Meyerowitz E.M.;
RT "Regulation of SUP expression identifies multiple regulators involved in
RT Arabidopsis floral meristem development.";
RL Plant Cell 12:1607-1618(2000).
RN [8]
RP FUNCTION, AND IDENTIFICATION OF THE CARBOXY-TERMINAL REPRESSION DOMAIN.
RX PubMed=11943180; DOI=10.1016/s0014-5793(02)02435-3;
RA Hiratsu K., Ohta M., Matsui K., Ohme-Takagi M.;
RT "The SUPERMAN protein is an active repressor whose carboxy-terminal
RT repression domain is required for the development of normal flowers.";
RL FEBS Lett. 514:351-354(2002).
RN [9]
RP INDUCTION.
RX PubMed=11898023; DOI=10.1038/nature731;
RA Jackson J.P., Lindroth A.M., Cao X., Jacobsen S.E.;
RT "Control of CpNpG DNA methylation by the KRYPTONITE histone H3
RT methyltransferase.";
RL Nature 416:556-560(2002).
RN [10]
RP STRUCTURE BY NMR OF 41-79.
RX PubMed=12616630; DOI=10.1002/cbic.200390028;
RA Isernia C., Bucci E., Leone M., Zaccaro L., Di Lello P., Digilio G.,
RA Esposito S., Saviano M., Di Blasio B., Pedone C., Pedone P.V.,
RA Fattorusso R.;
RT "NMR structure of the single QALGGH zinc finger domain from the Arabidopsis
RT thaliana SUPERMAN protein.";
RL ChemBioChem 4:171-180(2003).
CC -!- FUNCTION: Probable transcriptional regulator considered as cadastral
CC protein that acts indirectly to prevent the B class homeotic proteins
CC APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl.
CC Principal function is to balance cell proliferation in the third and
CC fourth whorls of developing flowers thereby maintaining the boundary
CC between stamens and carpels. May fulfill this role by repressing genes
CC implicated in cell division. Plays equally a role in the determinacy of
CC the floral meristem. Is also required for normal ovule development.
CC {ECO:0000269|PubMed:11006335, ECO:0000269|PubMed:11943180,
CC ECO:0000269|PubMed:12324589, ECO:0000269|PubMed:1352237,
CC ECO:0000269|PubMed:7477325}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed early in the third whorl, and later
CC becomes localized at the boundary between the stamen and carpel whorls.
CC Also detected in developing ovule primordia.
CC -!- INDUCTION: Repressed by KRYPTONITE/SUVH4, member of the histone H3-K9
CC methyltransferase family that contributes with other factors to the
CC CpNpG methylation of the SUP gene resulting in its silencing. The
CC alternative epigenetic states of the SUPERMAN locus have been called
CC Clark Kent alleles. Positively regulated at an early stage of
CC development by LEAFY and by B class homeotic proteins APETALA3 and
CC PISTILLATA. Later expression is maintained by both the B class homeotic
CC proteins and the C class homeotic protein AGAMOUS. These two steps of
CC regulation require the intervention of additional factors.
CC {ECO:0000269|PubMed:11898023}.
CC -!- DOMAIN: Contains two overlapping leucine-zipper-like motifs at the C-
CC terminal region, which might serve as a site for protein-protein
CC interaction. In this domain, a slightly degenerated ERF-associated
CC amphiphilic repression (EAR) motif seems to be involved in the activity
CC of transcriptional repression.
CC -!- MISCELLANEOUS: Mutations in SUP cause the ectopic expression of
CC APETALA3 leading to the development of extra stamens at the expense of
CC the central carpels of the flower.
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DR EMBL; U38946; AAC49116.1; -; Genomic_DNA.
DR EMBL; AB025608; BAA95724.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76721.1; -; Genomic_DNA.
DR EMBL; DQ056605; AAY78753.1; -; mRNA.
DR PIR; S60325; S60325.
DR RefSeq; NP_188954.1; NM_113214.2.
DR PDB; 1NJQ; NMR; -; A=42-78.
DR PDB; 2L1O; NMR; -; A=42-78.
DR PDBsum; 1NJQ; -.
DR PDBsum; 2L1O; -.
DR AlphaFoldDB; Q38895; -.
DR BMRB; Q38895; -.
DR SMR; Q38895; -.
DR BioGRID; 7220; 10.
DR IntAct; Q38895; 10.
DR STRING; 3702.AT3G23130.1; -.
DR PaxDb; Q38895; -.
DR PRIDE; Q38895; -.
DR EnsemblPlants; AT3G23130.1; AT3G23130.1; AT3G23130.
DR GeneID; 821888; -.
DR Gramene; AT3G23130.1; AT3G23130.1; AT3G23130.
DR KEGG; ath:AT3G23130; -.
DR Araport; AT3G23130; -.
DR TAIR; locus:2086268; AT3G23130.
DR eggNOG; ENOG502RXP0; Eukaryota.
DR HOGENOM; CLU_068782_0_2_1; -.
DR InParanoid; Q38895; -.
DR OMA; ENACETM; -.
DR OrthoDB; 1376259at2759; -.
DR PhylomeDB; Q38895; -.
DR EvolutionaryTrace; Q38895; -.
DR PRO; PR:Q38895; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q38895; baseline and differential.
DR Genevisible; Q38895; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0090691; P:formation of plant organ boundary; IMP:TAIR.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IMP:TAIR.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:TAIR.
DR GO; GO:0008361; P:regulation of cell size; IMP:TAIR.
DR GO; GO:0010093; P:specification of floral organ identity; IMP:CACAO.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; Differentiation; DNA-binding;
KW Flowering; Metal-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..204
FT /note="Transcriptional regulator SUPERMAN"
FT /id="PRO_0000047839"
FT ZN_FING 47..69
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 78..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 153..204
FT /note="Leucine-zipper-like"
FT MOTIF 195..199
FT /note="EAR-like (transcriptional repression)"
FT MUTAGEN 63
FT /note="G->D: In sup-3."
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:1NJQ"
FT TURN 50..52
FT /evidence="ECO:0007829|PDB:1NJQ"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:1NJQ"
FT HELIX 59..67
FT /evidence="ECO:0007829|PDB:1NJQ"
FT TURN 74..77
FT /evidence="ECO:0007829|PDB:1NJQ"
SQ SEQUENCE 204 AA; 23084 MW; 2CF36E29A821F76E CRC64;
MERSNSIELR NSFYGRARTS PWSYGDYDNC QQDHDYLLGF SWPPRSYTCS FCKREFRSAQ
ALGGHMNVHR RDRARLRLQQ SPSSSSTPSP PYPNPNYSYS TMANSPPPHH SPLTLFPTLS
PPSSPRYRAG LIRSLSPKSK HTPENACKTK KSSLLVEAGE ATRFTSKDAC KILRNDEIIS
LELEIGLINE SEQDLDLELR LGFA
