SURE_XYLFA
ID SURE_XYLFA Reviewed; 262 AA.
AC Q9PF20;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=5'-nucleotidase SurE {ECO:0000255|HAMAP-Rule:MF_00060};
DE EC=3.1.3.5 {ECO:0000255|HAMAP-Rule:MF_00060};
DE AltName: Full=Nucleoside 5'-monophosphate phosphohydrolase {ECO:0000255|HAMAP-Rule:MF_00060};
GN Name=surE {ECO:0000255|HAMAP-Rule:MF_00060}; OrderedLocusNames=XF_0858;
OS Xylella fastidiosa (strain 9a5c).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xylella.
OX NCBI_TaxID=160492;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9a5c;
RX PubMed=10910347; DOI=10.1038/35018003;
RA Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M.,
RA Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S.,
RA Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S.,
RA Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H.,
RA Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M.,
RA Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H.,
RA Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S.,
RA Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H.,
RA Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D.,
RA Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E.,
RA Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F.,
RA Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N.,
RA Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F.,
RA Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y.,
RA Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O.,
RA Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A.,
RA de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R.,
RA Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B.,
RA Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G.,
RA Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M.,
RA da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z.,
RA Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D.,
RA Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S.,
RA Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.;
RT "The genome sequence of the plant pathogen Xylella fastidiosa.";
RL Nature 406:151-159(2000).
CC -!- FUNCTION: Nucleotidase that shows phosphatase activity on nucleoside
CC 5'-monophosphates. {ECO:0000255|HAMAP-Rule:MF_00060}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +
CC phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00060};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00060};
CC Note=Binds 1 divalent metal cation per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00060};
CC -!- INTERACTION:
CC Q9PF20; Q9PF20: surE; NbExp=2; IntAct=EBI-7531616, EBI-7531616;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00060}.
CC -!- SIMILARITY: Belongs to the SurE nucleotidase family.
CC {ECO:0000255|HAMAP-Rule:MF_00060}.
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DR EMBL; AE003849; AAF83668.1; -; Genomic_DNA.
DR PIR; D82754; D82754.
DR RefSeq; WP_010893378.1; NC_002488.3.
DR PDB; 5KSQ; X-ray; 2.63 A; A/B=1-262.
DR PDB; 5KSR; X-ray; 1.96 A; A/B/C/D=1-262.
DR PDB; 5KSS; X-ray; 2.82 A; A/B=1-262.
DR PDB; 5KST; X-ray; 2.76 A; A/B/C/D=1-262.
DR PDBsum; 5KSQ; -.
DR PDBsum; 5KSR; -.
DR PDBsum; 5KSS; -.
DR PDBsum; 5KST; -.
DR AlphaFoldDB; Q9PF20; -.
DR SMR; Q9PF20; -.
DR MINT; Q9PF20; -.
DR STRING; 160492.XF_0858; -.
DR EnsemblBacteria; AAF83668; AAF83668; XF_0858.
DR KEGG; xfa:XF_0858; -.
DR eggNOG; COG0496; Bacteria.
DR HOGENOM; CLU_045192_1_2_6; -.
DR OMA; DCVHIAL; -.
DR Proteomes; UP000000812; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0106411; F:XMP 5'-nucleosidase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.1210.10; -; 1.
DR HAMAP; MF_00060; SurE; 1.
DR InterPro; IPR030048; SurE.
DR InterPro; IPR002828; SurE-like_Pase/nucleotidase.
DR InterPro; IPR036523; SurE-like_sf.
DR PANTHER; PTHR30457; PTHR30457; 1.
DR Pfam; PF01975; SurE; 1.
DR SUPFAM; SSF64167; SSF64167; 1.
DR TIGRFAMs; TIGR00087; surE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Metal-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..262
FT /note="5'-nucleotidase SurE"
FT /id="PRO_0000111857"
FT BINDING 8
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00060"
FT BINDING 9
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00060"
FT BINDING 40
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00060"
FT BINDING 92
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00060"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 14..25
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 29..37
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 54..58
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 68..76
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 85..94
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 98..100
FT /evidence="ECO:0007829|PDB:5KSR"
FT TURN 101..103
FT /evidence="ECO:0007829|PDB:5KSS"
FT HELIX 105..115
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 138..154
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 159..170
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 192..196
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 202..206
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 220..225
FT /evidence="ECO:0007829|PDB:5KSR"
FT STRAND 229..234
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 241..243
FT /evidence="ECO:0007829|PDB:5KSR"
FT HELIX 244..258
FT /evidence="ECO:0007829|PDB:5KSR"
SQ SEQUENCE 262 AA; 28391 MW; 68907928DDE937B5 CRC64;
MRVLVSNDDG VDAPGIKILA DALRNAGHEV MVVAPDRDRS GASNSLTLDT PIRAKQIDMH
TYSVAGTPTD CVHLALTGLL NYDPDIVVSG INNTGNLGDD VIYSGTVSAA MEGRFLGLPA
VAVSLVTLYR EGQQAPQYET AAHAAINIVA QLKTDPLPAD TILNVNVPDV TWQQMRGFKV
TRLGNRHRSA PCLTQTDPRG HTIYWIGPAG PEQDAGPGTD FDAVRNTYIS ITPIHVDLTR
YQALENVTRW TDRLTAHMDW PT
