SUS2_ARATH
ID SUS2_ARATH Reviewed; 807 AA.
AC Q00917; F4K4S0; Q9FJ20;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Sucrose synthase 2;
DE Short=AtSUS2;
DE EC=2.4.1.13;
DE AltName: Full=Sucrose-UDP glucosyltransferase 2;
GN Name=SUS2; Synonyms=SSA; OrderedLocusNames=At5g49190; ORFNames=K21P3.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=1531031; DOI=10.1007/bf00018465;
RA Chopra S., Del-Favero J., Dolferus R., Jacobs M.;
RT "Sucrose synthase of Arabidopsis: genomic cloning and sequence
RT characterization.";
RL Plant Mol. Biol. 18:131-134(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP INDUCTION.
RX PubMed=10567234; DOI=10.1042/bj3440503;
RA Dejardin A., Sokolov L.N., Kleczkowski L.A.;
RT "Sugar/osmoticum levels modulate differential abscisic acid-independent
RT expression of two stress-responsive sucrose synthase genes in
RT Arabidopsis.";
RL Biochem. J. 344:503-509(1999).
RN [5]
RP GENE FAMILY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=14739263; DOI=10.1093/jxb/erh047;
RA Baud S., Vaultier M.N., Rochat C.;
RT "Structure and expression profile of the sucrose synthase multigene family
RT in Arabidopsis.";
RL J. Exp. Bot. 55:397-409(2004).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=17257168; DOI=10.1111/j.1365-313x.2006.03011.x;
RA Bieniawska Z., Paul Barratt D.H., Garlick A.P., Thole V., Kruger N.J.,
RA Martin C., Zrenner R., Smith A.M.;
RT "Analysis of the sucrose synthase gene family in Arabidopsis.";
RL Plant J. 49:810-828(2007).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=18635527; DOI=10.1093/jxb/ern180;
RA Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.;
RT "Localization of sucrose synthase in developing seed and siliques of
RT Arabidopsis thaliana reveals diverse roles for SUS during development.";
RL J. Exp. Bot. 59:3283-3295(2008).
RN [8]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=18701523; DOI=10.1093/pcp/pcn117;
RA Angeles-Nunez J.G., Kronenberger J., Wuilleme S., Lepiniec L., Rochat C.;
RT "Study of AtSUS2 localization in seeds reveals a strong association with
RT plastids.";
RL Plant Cell Physiol. 49:1621-1626(2008).
RN [9]
RP INDUCTION.
RX PubMed=19207209; DOI=10.1111/j.1365-313x.2009.03817.x;
RA Yamamoto A., Kagaya Y., Toyoshima R., Kagaya M., Takeda S., Hattori T.;
RT "Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by
RT interacting with seed-specific ABRE-binding factors.";
RL Plant J. 58:843-856(2009).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20559653; DOI=10.1007/s00425-010-1207-9;
RA Angeles-Nunez J.G., Tiessen A.;
RT "Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis and
RT direct carbon towards starch synthesis in developing seeds.";
RL Planta 232:701-718(2010).
RN [11]
RP FUNCTION, AND INDUCTION BY LEC2.
RX PubMed=22228409; DOI=10.1007/s11103-011-9871-0;
RA Angeles-Nunez J.G., Tiessen A.;
RT "Regulation of AtSUS2 and AtSUS3 by glucose and the transcription factor
RT LEC2 in different tissues and at different stages of Arabidopsis seed
RT development.";
RL Plant Mol. Biol. 78:377-392(2012).
CC -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC fructose for various metabolic pathways. Modulates metabolic
CC homeostasis and directs carbon towards starch synthesis in developing
CC seeds. {ECO:0000269|PubMed:20559653, ECO:0000269|PubMed:22228409}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18701523}. Plastid
CC membrane {ECO:0000269|PubMed:18701523}; Peripheral membrane protein
CC {ECO:0000269|PubMed:18701523}. Note=Associated with plastid membranes
CC during the maturation phase of the seed.
CC -!- TISSUE SPECIFICITY: Detected in the whole plant but at lower levels.
CC Predominantly expressed in developing siliques. Also detected in the
CC root tip. Detected in the embryo, endosperm and seed coat (at the
CC protein level). {ECO:0000269|PubMed:14739263,
CC ECO:0000269|PubMed:17257168, ECO:0000269|PubMed:18635527,
CC ECO:0000269|PubMed:18701523}.
CC -!- DEVELOPMENTAL STAGE: Specifically and highly expressed during seed
CC maturation at 12 days after flowering (at the protein level).
CC {ECO:0000269|PubMed:14739263, ECO:0000269|PubMed:17257168}.
CC -!- INDUCTION: By anaerobic stress and by glucose. Positively regulated by
CC the NF-Y/HAP transcription factor complex at least composed of
CC NFYB9/LEC1 or NFYB6/L1L and NFYC2/HAP5B in association with
CC DPBF2/BZIP67. Positively regulated by LEC2.
CC {ECO:0000269|PubMed:10567234, ECO:0000269|PubMed:19207209,
CC ECO:0000269|PubMed:22228409}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Diminution of the starch
CC content of developing seeds and increased lipid accumulation early
CC during seed development. {ECO:0000269|PubMed:14739263,
CC ECO:0000269|PubMed:20559653}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC synthase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10337.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA43303.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; X60987; CAA43303.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB016872; BAB10337.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95780.1; -; Genomic_DNA.
DR PIR; S19125; YUMU.
DR RefSeq; NP_199730.1; NM_124296.3.
DR AlphaFoldDB; Q00917; -.
DR SMR; Q00917; -.
DR BioGRID; 20224; 1.
DR IntAct; Q00917; 1.
DR STRING; 3702.AT5G49190.1; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; Q00917; -.
DR PRIDE; Q00917; -.
DR ProteomicsDB; 228403; -.
DR EnsemblPlants; AT5G49190.1; AT5G49190.1; AT5G49190.
DR GeneID; 834978; -.
DR Gramene; AT5G49190.1; AT5G49190.1; AT5G49190.
DR KEGG; ath:AT5G49190; -.
DR Araport; AT5G49190; -.
DR TAIR; locus:2155894; AT5G49190.
DR eggNOG; KOG0853; Eukaryota.
DR HOGENOM; CLU_019158_1_0_1; -.
DR InParanoid; Q00917; -.
DR OrthoDB; 170782at2759; -.
DR BRENDA; 2.4.1.13; 399.
DR PRO; PR:Q00917; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q00917; baseline and differential.
DR Genevisible; Q00917; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0042170; C:plastid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016157; F:sucrose synthase activity; IMP:TAIR.
DR GO; GO:0001666; P:response to hypoxia; IEP:TAIR.
DR GO; GO:0010431; P:seed maturation; IMP:TAIR.
DR GO; GO:0005982; P:starch metabolic process; IMP:TAIR.
DR GO; GO:0005985; P:sucrose metabolic process; IMP:TAIR.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR000368; Sucrose_synth.
DR InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR PANTHER; PTHR45839; PTHR45839; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR TIGRFAMs; TIGR02470; sucr_synth; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Glycosyltransferase; Membrane; Plastid; Reference proteome;
KW Stress response; Transferase.
FT CHAIN 1..807
FT /note="Sucrose synthase 2"
FT /id="PRO_0000204645"
FT REGION 274..752
FT /note="GT-B glycosyltransferase"
FT /evidence="ECO:0000250"
SQ SEQUENCE 807 AA; 92064 MW; 48391FF65C4584DB CRC64;
MPTGRFETMR EWVYDAISAQ RNELLSLFSR YVAQGKGILQ SHQLIDEFLK TVKVDGTLED
LNKSPFMKVL QSAEEAIVLP PFVALAIRPR PGVREYVRVN VYELSVDHLT VSEYLRFKEE
LVNGHANGDY LLELDFEPFN ATLPRPTRSS SIGNGVQFLN RHLSSIMFRN KESMEPLLEF
LRTHKHDGRP MMLNDRIQNI PILQGALARA EEFLSKLPLA TPYSEFEFEL QGMGFERGWG
DTAQKVSEMV HLLLDILQAP DPSVLETFLG RIPMVFNVVI LSPHGYFGQA NVLGLPDTGG
QVVYILDQVR ALENEMLLRI QKQGLEVIPK ILIVTRLLPE AKGTTCNQRL ERVSGTEHAH
ILRIPFRTEK GILRKWISRF DVWPYLETFA EDASNEISAE LQGVPNLIIG NYSDGNLVAS
LLASKLGVIQ CNIAHALEKT KYPESDIYWR NHEDKYHFSS QFTADLIAMN NADFIITSTY
QEIAGSKNNV GQYESHTAFT MPGLYRVVHG IDVFDPKFNI VSPGADMTIY FPYSDKERRL
TALHESIEEL LFSAEQNDEH VGLLSDQSKP IIFSMARLDR VKNLTGLVEC YAKNSKLREL
ANLVIVGGYI DENQSRDREE MAEIQKMHSL IEQYDLHGEF RWIAAQMNRA RNGELYRYIA
DTKGVFVQPA FYEAFGLTVV ESMTCALPTF ATCHGGPAEI IENGVSGFHI DPYHPDQVAA
TLVSFFETCN TNPNHWVKIS EGGLKRIYER YTWKKYSERL LTLAGVYAFW KHVSKLERRE
TRRYLEMFYS LKFRDLANSI PLATDEN
