SUS2_ORYSJ
ID SUS2_ORYSJ Reviewed; 808 AA.
AC P30298; A0A0P0WU51; A1YQI8; H6TFZ1; Q0DDW2; Q53X21; Q69V32;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Sucrose synthase 2;
DE Short=OsSUS2;
DE EC=2.4.1.13;
DE AltName: Full=Sucrose synthase 1;
DE Short=RSs1;
DE AltName: Full=Sucrose-UDP glucosyltransferase 2;
GN Name=SUS2; Synonyms=RSS1, RSUS2;
GN OrderedLocusNames=Os06g0194900, LOC_Os06g09450; ORFNames=P0648E08.22;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Tainung 67; TISSUE=Seed;
RX PubMed=1386537; DOI=10.1007/bf00027086;
RA Wang M.-B., Boulter D., Gatehouse J.A.;
RT "A complete sequence of the rice sucrose synthase-1 (RSs1) gene.";
RL Plant Mol. Biol. 19:881-885(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Tainung 67; TISSUE=Seed;
RX PubMed=9063969; DOI=10.1271/bbb.60.233;
RA Huang J.W., Chen J.T., Yu W.P., Shyur L.F., Wang A.Y., Sung H.Y., Lee P.D.,
RA Su J.C.;
RT "Complete structures of three rice sucrose synthase isogenes and
RT differential regulation of their expressions.";
RL Biosci. Biotechnol. Biochem. 60:233-239(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. NATO; TISSUE=Seed;
RA Odegard W., Liu J.J., de Lumen B.O.;
RT "Isolation and sequence of a sucrose synthase cDNA from developing rice
RT seeds.";
RL Plant Sci. 113:67-78(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA Yoon U.H., Kim Y.H.;
RT "Molecular cloning of sucrose synthase 2 genes in rice seeds.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Nipponbare;
RX PubMed=21533550; DOI=10.1007/s10059-011-1038-y;
RA Cho J.I., Kim H.B., Kim C.Y., Hahn T.R., Jeon J.S.;
RT "Identification and characterization of the duplicate rice sucrose synthase
RT genes OsSUS5 and OsSUS7 which are associated with the plasma membrane.";
RL Mol. Cells 31:553-561(2011).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [8]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP SUBUNIT.
RX PubMed=9784845; DOI=10.1080/15216549800203612;
RA Huang D.Y., Wang A.-Y.;
RT "Purification and characterization of sucrose synthase isozymes from
RT etiolated rice seedlings.";
RL Biochem. Mol. Biol. Int. 46:107-113(1998).
RN [11]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10555303; DOI=10.1093/oxfordjournals.pcp.a029608;
RA Wang A.-Y., Kao M.-H., Yang W.-H., Sayion Y., Liu L.-F., Lee P.-D.,
RA Su J.-C.;
RT "Differentially and developmentally regulated expression of three rice
RT sucrose synthase genes.";
RL Plant Cell Physiol. 40:800-807(1999).
RN [12]
RP FUNCTION, ACTIVITY REGULATION, PHOSPHORYLATION AT SER-10, AND MUTAGENESIS
RP OF SER-10.
RX PubMed=11910009; DOI=10.1105/tpc.010454;
RA Asano T., Kunieda N., Omura Y., Ibe H., Kawasaki T., Takano M., Sato M.,
RA Furuhashi H., Mujin T., Takaiwa F., Wu Cy C.Y., Tada Y., Satozawa T.,
RA Sakamoto M., Shimada H.;
RT "Rice SPK, a calmodulin-like domain protein kinase, is required for storage
RT product accumulation during seed development: phosphorylation of sucrose
RT synthase is a possible factor.";
RL Plant Cell 14:619-628(2002).
RN [13]
RP INDUCTION BY SUGARS.
RA Liao Y.-C., Wang A.-Y.;
RT "Sugar-modulated gene expression of sucrose synthase in suspension-cultured
RT cells of rice.";
RL Physiol. Plantarum 118:319-327(2003).
RN [14]
RP DEVELOPMENTAL STAGE.
RX PubMed=15701658; DOI=10.1093/pcp/pci066;
RA Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H.,
RA Terao T., Ishii R., Ohsugi R., Yamagishi T.;
RT "Expression patterns of genes encoding carbohydrate-metabolizing enzymes
RT and their relationship to grain filling in rice (Oryza sativa L.):
RT comparison of caryopses located at different positions in a panicle.";
RL Plant Cell Physiol. 46:620-628(2005).
RN [15]
RP GENE FAMILY, AND INDUCTION BY SUBMERGENCE.
RX DOI=10.1016/j.plantsci.2008.02.009;
RA Hirose T., Scofield G.N., Terao T.;
RT "An expression analysis profile for the entire sucrose synthase gene family
RT in rice.";
RL Plant Sci. 174:534-543(2008).
CC -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC fructose for various metabolic pathways (By similarity). Functions in
CC developing seeds by supplying substrates for the biosynthesis of
CC storage products (PubMed:11910009). {ECO:0000250,
CC ECO:0000269|PubMed:11910009}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC -!- ACTIVITY REGULATION: Activated by phosphorylation at Ser-10 by CPK23.
CC {ECO:0000269|PubMed:11910009}.
CC -!- SUBUNIT: Homotetramer or heterotetramer with SUS1.
CC {ECO:0000269|PubMed:9784845}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the leaf tissues.
CC Expressed in seeds, and at lower levels in roots. Expressed in leaf
CC mesophyll and phloem (at protein level). {ECO:0000269|PubMed:10555303,
CC ECO:0000269|PubMed:21533550}.
CC -!- DEVELOPMENTAL STAGE: Expressed early in seed development with a peak at
CC 6 days after pollination (DAP) and then decreases slightly (at protein
CC level). In the caryopse, slightly expressed during the pre-storage
CC phase and was strongly expressed from the early to middle storage
CC phase. {ECO:0000269|PubMed:10555303, ECO:0000269|PubMed:15701658,
CC ECO:0000269|PubMed:21533550}.
CC -!- INDUCTION: By anaerobic stress. Up-regulated by sucrose, fructose and
CC glucose and by sugar starvation. By submergence. {ECO:0000269|Ref.13,
CC ECO:0000269|Ref.15}.
CC -!- PTM: Phosphorylated at Ser-10 by CPK23 in developing seeds.
CC {ECO:0000269|PubMed:11910009}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC synthase subfamily. {ECO:0000305}.
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DR EMBL; L39940; AAL31375.1; -; Genomic_DNA.
DR EMBL; X64770; CAA46017.1; -; Genomic_DNA.
DR EMBL; Z15028; CAA78747.1; -; mRNA.
DR EMBL; EF122480; ABL74567.1; -; mRNA.
DR EMBL; EF122481; ABL74568.1; -; mRNA.
DR EMBL; HQ895720; AEX32875.1; -; mRNA.
DR EMBL; AP004280; BAD35646.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF18961.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS96603.1; -; Genomic_DNA.
DR EMBL; AK100546; BAG94649.1; -; mRNA.
DR PIR; S23543; S23543.
DR RefSeq; XP_015643025.1; XM_015787539.1.
DR RefSeq; XP_015643026.1; XM_015787540.1.
DR RefSeq; XP_015643027.1; XM_015787541.1.
DR AlphaFoldDB; P30298; -.
DR SMR; P30298; -.
DR STRING; 4530.OS06T0194900-03; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR iPTMnet; P30298; -.
DR PaxDb; P30298; -.
DR PRIDE; P30298; -.
DR EnsemblPlants; Os06t0194900-01; Os06t0194900-01; Os06g0194900.
DR EnsemblPlants; Os06t0194900-02; Os06t0194900-02; Os06g0194900.
DR EnsemblPlants; Os06t0194900-03; Os06t0194900-03; Os06g0194900.
DR GeneID; 4340386; -.
DR Gramene; Os06t0194900-01; Os06t0194900-01; Os06g0194900.
DR Gramene; Os06t0194900-02; Os06t0194900-02; Os06g0194900.
DR Gramene; Os06t0194900-03; Os06t0194900-03; Os06g0194900.
DR KEGG; osa:4340386; -.
DR eggNOG; KOG0853; Eukaryota.
DR InParanoid; P30298; -.
DR OMA; DMSVYFP; -.
DR OrthoDB; 153947at2759; -.
DR BRENDA; 2.4.1.13; 4460.
DR PlantReactome; R-OSA-1119452; Galactose degradation II.
DR PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR ExpressionAtlas; P30298; baseline and differential.
DR Genevisible; P30298; OS.
DR GO; GO:0016157; F:sucrose synthase activity; IBA:GO_Central.
DR GO; GO:0051262; P:protein tetramerization; IPI:UniProtKB.
DR GO; GO:0010431; P:seed maturation; IMP:UniProtKB.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:InterPro.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR000368; Sucrose_synth.
DR InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR PANTHER; PTHR45839; PTHR45839; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR TIGRFAMs; TIGR02470; sucr_synth; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Phosphoprotein; Reference proteome; Stress response;
KW Transferase.
FT CHAIN 1..808
FT /note="Sucrose synthase 2"
FT /id="PRO_0000204655"
FT REGION 272..749
FT /note="GT-B glycosyltransferase"
FT /evidence="ECO:0000250"
FT MOD_RES 10
FT /note="Phosphoserine; by CPK"
FT /evidence="ECO:0000269|PubMed:11910009"
FT MUTAGEN 10
FT /note="S->V: Loss of phosphorylation."
FT CONFLICT 128
FT /note="F -> L (in Ref. 4; ABL74568)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="L -> P (in Ref. 3; CAA78747)"
FT /evidence="ECO:0000305"
FT CONFLICT 577
FT /note="D -> G (in Ref. 5; AEX32875)"
FT /evidence="ECO:0000305"
FT CONFLICT 649..652
FT /note="NGEL -> TGEM (in Ref. 5; AEX32875)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 808 AA; 92129 MW; 93E24B2571EB1DAE CRC64;
MAAKLARLHS LRERLGATFS SHPNELIALF SRYVNQGKGM LQRHQLLAEF DALIEADKEK
YAPFEDILRA AQEAIVLPPW VALAIRPRPG VWDYIRVNVS ELAVEELSVS EYLAFKEQLV
DGHTNSNFVL ELDFEPFNAS FPRPSMSKSI GNGVQFLNRH LSSKLFQDKE SLYPLLNFLK
AHNHKGTTMM LNDRIQSLRG LQSSLRKAEE YLMGIPQDTP YSEFNHRFQE LGLEKGWGDC
AKRVLDTIHL LLDLLEAPDP ANLEKFLGTI PMMFNVVILS PHGYFAQSNV LGYPDTGGQV
VYILDQVRAL ENEMLLRIKQ QGLDITPKIL IVTRLLPDAV GTTCGQRVEK VIGTEHTDIL
RVPFRSENGI LRKWISRFDV WPFLETYTED VANEIMREMQ AKPDLIIGNY SDGNLVATLL
AHKLGVTQCT IAHALEKTKY PNSDIYLDKF DSQYHFSCQF TADLIAMNHT DFIITSTFQE
IAGSKDTVGQ YESHIAFTLP GLYRVVHGID VFDPKFNIVS PGADMSVYFP YTEADKRLTA
FHPEIEELLY SEVENDEHKF VLKDKNKPII FSMARLDRVK NMTGLVEMYG KNAHLRDLAN
LVIVCGDHGN QSKDREEQAE FKKMYGLIDQ YKLKGHIRWI SAQMNRVRNG ELYRYICDTK
GVFVQPAFYE AFGLTVIEAM TCGLPTIATC HGGPAEIIVD GVSGLHIDPY HSDKAADILV
NFFEKCKQDS TYWDNISQGG LQRIYEKYTW KLYSERLMTL TGVYGFWKYV SNLERRETRR
YIEMFYALKY RSLASAVPLA VDGESTSK
