ID   SUS3_ORYSJ              Reviewed;         816 AA.
AC   Q43009; A0A0P0X8V1; H6TFZ2; Q0D4N1; Q8GS23;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 2.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Sucrose synthase 3;
DE            Short=OsSUS3;
DE            Short=RSs3;
DE            EC=2.4.1.13;
DE   AltName: Full=Sucrose-UDP glucosyltransferase 3;
GN   Name=SUS3; Synonyms=RSS3, RSUS3;
GN   OrderedLocusNames=Os07g0616800, LOC_Os07g42490;
GN   ORFNames=B1056G08.118, OsJ_25133, P0552F09.101;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Tainung 67; TISSUE=Leaf, and Root;
RX   PubMed=9063969; DOI=10.1271/bbb.60.233;
RA   Huang J.W., Chen J.T., Yu W.P., Shyur L.F., Wang A.Y., Sung H.Y., Lee P.D.,
RA   Su J.C.;
RT   "Complete structures of three rice sucrose synthase isogenes and
RT   differential regulation of their expressions.";
RL   Biosci. Biotechnol. Biochem. 60:233-239(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA   Yoon U.H., Kim Y.H.;
RT   "Molecular cloning of sucrose synthase 3 genes in rice seeds.";
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=21533550; DOI=10.1007/s10059-011-1038-y;
RA   Cho J.I., Kim H.B., Kim C.Y., Hahn T.R., Jeon J.S.;
RT   "Identification and characterization of the duplicate rice sucrose synthase
RT   genes OsSUS5 and OsSUS7 which are associated with the plasma membrane.";
RL   Mol. Cells 31:553-561(2011).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [9]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=10555303; DOI=10.1093/oxfordjournals.pcp.a029608;
RA   Wang A.-Y., Kao M.-H., Yang W.-H., Sayion Y., Liu L.-F., Lee P.-D.,
RA   Su J.-C.;
RT   "Differentially and developmentally regulated expression of three rice
RT   sucrose synthase genes.";
RL   Plant Cell Physiol. 40:800-807(1999).
RN   [10]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=15701658; DOI=10.1093/pcp/pci066;
RA   Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H.,
RA   Terao T., Ishii R., Ohsugi R., Yamagishi T.;
RT   "Expression patterns of genes encoding carbohydrate-metabolizing enzymes
RT   and their relationship to grain filling in rice (Oryza sativa L.):
RT   comparison of caryopses located at different positions in a panicle.";
RL   Plant Cell Physiol. 46:620-628(2005).
RN   [11]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   DOI=10.1016/j.plantsci.2008.02.009;
RA   Hirose T., Scofield G.N., Terao T.;
RT   "An expression analysis profile for the entire sucrose synthase gene family
RT   in rice.";
RL   Plant Sci. 174:534-543(2008).
CC   -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC       fructose for various metabolic pathways. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC         diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC         ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC   -!- TISSUE SPECIFICITY: Expressed in parenchymatous tissues, aleurone
CC       layers and cell surrounding the vascular tissue in seeds (at protein
CC       level). Predominantly expressed in caryopses.
CC       {ECO:0000269|PubMed:10555303}.
CC   -!- DEVELOPMENTAL STAGE: Not expressed in seed development before 3 days
CC       after pollination (DAP), reaches a peak at 6 DAP and then decreases
CC       slightly (at protein level). In the caryopse, expressed specifically
CC       from the early to middle storage stage. {ECO:0000269|PubMed:10555303,
CC       ECO:0000269|PubMed:15701658, ECO:0000269|PubMed:21533550}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC       synthase subfamily. {ECO:0000305}.
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DR   EMBL; L03366; AAC41682.1; -; Genomic_DNA.
DR   EMBL; EF122474; ABL74561.1; -; mRNA.
DR   EMBL; HQ895721; AEX32876.1; -; mRNA.
DR   EMBL; AP004308; BAC16012.1; -; Genomic_DNA.
DR   EMBL; AP004988; BAC21489.1; -; Genomic_DNA.
DR   EMBL; AP008213; BAF22192.1; -; Genomic_DNA.
DR   EMBL; AP014963; BAT02657.1; -; Genomic_DNA.
DR   EMBL; CM000144; EAZ40663.1; -; Genomic_DNA.
DR   EMBL; AK100306; BAG94543.1; -; mRNA.
DR   RefSeq; XP_015644807.1; XM_015789321.1.
DR   AlphaFoldDB; Q43009; -.
DR   SMR; Q43009; -.
DR   STRING; 4530.OS07T0616800-01; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   iPTMnet; Q43009; -.
DR   PaxDb; Q43009; -.
DR   PRIDE; Q43009; -.
DR   EnsemblPlants; Os07t0616800-01; Os07t0616800-01; Os07g0616800.
DR   GeneID; 4343910; -.
DR   Gramene; Os07t0616800-01; Os07t0616800-01; Os07g0616800.
DR   KEGG; osa:4343910; -.
DR   eggNOG; KOG0853; Eukaryota.
DR   HOGENOM; CLU_019158_1_0_1; -.
DR   InParanoid; Q43009; -.
DR   OMA; SYSAFEH; -.
DR   OrthoDB; 153947at2759; -.
DR   BRENDA; 2.4.1.13; 4460.
DR   PlantReactome; R-OSA-1119452; Galactose degradation II.
DR   PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000007752; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   Genevisible; Q43009; OS.
DR   GO; GO:0016157; F:sucrose synthase activity; IBA:GO_Central.
DR   GO; GO:0005985; P:sucrose metabolic process; IEA:InterPro.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR000368; Sucrose_synth.
DR   InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR   PANTHER; PTHR45839; PTHR45839; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF00862; Sucrose_synth; 1.
DR   TIGRFAMs; TIGR02470; sucr_synth; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..816
FT                   /note="Sucrose synthase 3"
FT                   /id="PRO_0000204657"
FT   REGION          280..757
FT                   /note="GT-B glycosyltransferase"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        11
FT                   /note="T -> N (in Ref. 1; AAC41682)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        425
FT                   /note="A -> R (in Ref. 1; AAC41682)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   816 AA;  93112 MW;  8A81C82705AD4831 CRC64;
     MGETTGERAL TRLHSMRERI GDSLSAHTNE LVAVFSRLVN QGKGMLQPHQ IIAEYNAAIP
     EGEREKLKDS ALEDVLRGAQ EAIVIPPWIA LAIRPRPGVW EYLRINVSQL GVEELSVPEY
     LQFKEQLVDG STQNNFVLEL DFEPFNASFP RPSLSKSIGN GVQFLNRHLS SKLFHDKESM
     YPLLNFLRAH NYKGMTMMLN DRIRSLDALQ GALRKAEKHL AGITADTPYS EFHHRFQELG
     LEKGWGDCAQ RVRETIHLLL DLLEAPEPSA LEKFLGTIPM VFNVVILSPH GYFAQANVLG
     YPDTGGQVVY ILDQVRAMEN EMLLRIKQQG LNITPRILIV TRLLPDAHGT TCGQRLEKVL
     GTEHTHILRV PFRTENGTVR KWISRFEVWP YLETYTDDVA HEISGELQAT PDLIIGNYSD
     GNLVACLLAH KLGVTHCTIA HALEKTKYPN SDLYWKKFED HYHFSCQFTA DLIAMNHADF
     IITSTFQEIA GNKETVGQYE SHMAFTMPGL YRVVHGIDVF DPKFNIVSPG ADMSIYFPFT
     ESQKRLTSLH LEIEELLFSD VENTEHKFVL KDKKKPIIFS MARLDHVKNL TGLVELYGRN
     PRLQELVNLV VVCGDHGKES KDKEEQAEFK KMFNLIEQYN LNGHIRWISA QMNRVRNGEL
     YRYICDMRGA FVQPALYEAF GLTVIEAMTC GLPTFATAYG GPAEIIVHGV SGYHIDPYQN
     DKASALLVEF FEKCQEDPNH WIKISQGGLQ RIEEKYTWKL YSERLMTLSG VYGFWKYVTN
     LDRRETRRYL EMLYALKYRK MATTVPLAIE GEASTK