ID   SUS4_ORYSJ              Reviewed;         809 AA.
AC   Q10LP5;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Sucrose synthase 4;
DE            Short=OsSUS4;
DE            EC=2.4.1.13;
DE   AltName: Full=Sucrose-UDP glucosyltransferase 4;
GN   Name=SUS4; OrderedLocusNames=Os03g0340500, LOC_Os03g22120;
GN   ORFNames=OsJ_10783;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=21533550; DOI=10.1007/s10059-011-1038-y;
RA   Cho J.I., Kim H.B., Kim C.Y., Hahn T.R., Jeon J.S.;
RT   "Identification and characterization of the duplicate rice sucrose synthase
RT   genes OsSUS5 and OsSUS7 which are associated with the plasma membrane.";
RL   Mol. Cells 31:553-561(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [8]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   DOI=10.1016/j.plantsci.2008.02.009;
RA   Hirose T., Scofield G.N., Terao T.;
RT   "An expression analysis profile for the entire sucrose synthase gene family
RT   in rice.";
RL   Plant Sci. 174:534-543(2008).
CC   -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC       fructose for various metabolic pathways. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC         diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC         ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in the leaf tissues and in
CC       caryopses. {ECO:0000269|PubMed:21533550, ECO:0000269|Ref.8}.
CC   -!- DEVELOPMENTAL STAGE: In the caryopse, slightly expressed during the
CC       middle storage phase and was strongly expressed at the later storage
CC       phase. {ECO:0000269|PubMed:21533550}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC       synthase subfamily. {ECO:0000305}.
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DR   EMBL; HQ895722; AEX32877.1; -; mRNA.
DR   EMBL; DP000009; ABF95853.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF11978.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS84118.1; -; Genomic_DNA.
DR   EMBL; CM000140; EAZ26864.1; -; Genomic_DNA.
DR   EMBL; AK099406; BAG94112.1; -; mRNA.
DR   EMBL; AK102158; BAG95417.1; -; mRNA.
DR   RefSeq; XP_015632177.1; XM_015776691.1.
DR   AlphaFoldDB; Q10LP5; -.
DR   SMR; Q10LP5; -.
DR   STRING; 4530.OS03T0340500-01; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   PaxDb; Q10LP5; -.
DR   PRIDE; Q10LP5; -.
DR   EnsemblPlants; Os03t0340500-01; Os03t0340500-01; Os03g0340500.
DR   GeneID; 4332788; -.
DR   Gramene; Os03t0340500-01; Os03t0340500-01; Os03g0340500.
DR   KEGG; osa:4332788; -.
DR   eggNOG; KOG0853; Eukaryota.
DR   HOGENOM; CLU_019158_1_0_1; -.
DR   InParanoid; Q10LP5; -.
DR   OMA; WISRFEI; -.
DR   OrthoDB; 170782at2759; -.
DR   BRENDA; 2.4.1.13; 4460.
DR   PlantReactome; R-OSA-1119452; Galactose degradation II.
DR   PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q10LP5; baseline and differential.
DR   Genevisible; Q10LP5; OS.
DR   GO; GO:0016157; F:sucrose synthase activity; IBA:GO_Central.
DR   GO; GO:0005985; P:sucrose metabolic process; IEA:InterPro.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR000368; Sucrose_synth.
DR   InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR   PANTHER; PTHR45839; PTHR45839; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF00862; Sucrose_synth; 1.
DR   TIGRFAMs; TIGR02470; sucr_synth; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..809
FT                   /note="Sucrose synthase 4"
FT                   /id="PRO_0000418806"
FT   REGION          275..753
FT                   /note="GT-B glycosyltransferase"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   809 AA;  92217 MW;  4D20B0F0E42A56DC CRC64;
     MSGPKLDRTP SIRDRVEDTL HAHRNELVAL LSKYVSQGKG ILQPHHILDA LDEVQSSGGR
     ALVEGPFLDV LRSAQEAIVL PPFVAIAVRP RPGVWEYVRV NVHELSVEQL TVSEYLRFKE
     ELVDGQYNDP YILELDFEPF NASVPRPNRS SSIGNGVQFL NRHLSSIMFR NKDCLEPLLD
     FLRGHRHKGH VMMLNDRIQS LGRLQSVLTK AEEHLSKLPA DTPYSQFAYK FQEWGLEKGW
     GDTAGYVLEM IHLLLDVLQA PDPSTLETFL GRIPMIFNVV VVSPHGYFGQ ANVLGLPDTG
     GQIVYILDQV RALENEMVLR LKKQGLDFTP KILIVTRLIP EAKGTSCNQR LERISGTQHT
     YILRVPFRNE NGILRKWISR FDVWPYLEKF AEDAAGEIAA ELQGTPDFII GNYSDGNLVA
     SLLSYKMGIT QCNIAHALEK TKYPDSDIYW TKYDEKYHFS CQFTADIIAM NNADFIITST
     YQEIAGSKNT VGQYESHTAF TLPGLYRIVH GIDVFDPKFN IVSPGADMSI YFPYTEKAKR
     LTSLHGSLEN LISDPEQNDE HIGHLDDRSK PILFSMARLD RVKNITGLVE AYAKNARLRE
     LVNLVVVAGY NDVKKSKDRE EIAEIEKMHE LIKTYNLFGQ FRWISAQTNR ARNGELYRYI
     ADTHGAFVQP AFYEAFGLTV VEAMTCGLPT FATVHGGPAE IIEHGISGFH IDPYHPDQAA
     NLIADFFEQC KQDPNHWVEV SNRGLQRIYE KYTWKIYSER LMTLAGVYGF WKYVSKLERR
     ETRRYLEMFY ILKFRELAKT VPLAVDEAH