ID   SUS5_ORYSJ              Reviewed;         855 AA.
AC   H6TFZ4; Q0JE91; Q7XNQ9;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2012, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Sucrose synthase 5;
DE            Short=OsSUS5;
DE            EC=2.4.1.13;
DE   AltName: Full=Sucrose-UDP glucosyltransferase 5;
GN   Name=SUS5; OrderedLocusNames=Os04g0309600, LOC_Os04g24430;
GN   ORFNames=OSJNBa0033H08.16;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, SUBCELLULAR LOCATION,
RP   AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=21533550; DOI=10.1007/s10059-011-1038-y;
RA   Cho J.I., Kim H.B., Kim C.Y., Hahn T.R., Jeon J.S.;
RT   "Identification and characterization of the duplicate rice sucrose synthase
RT   genes OsSUS5 and OsSUS7 which are associated with the plasma membrane.";
RL   Mol. Cells 31:553-561(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   GENE FAMILY.
RX   DOI=10.1016/j.plantsci.2008.02.009;
RA   Hirose T., Scofield G.N., Terao T.;
RT   "An expression analysis profile for the entire sucrose synthase gene family
RT   in rice.";
RL   Plant Sci. 174:534-543(2008).
CC   -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC       fructose for various metabolic pathways. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC         diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC         ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21533550}. Membrane
CC       {ECO:0000269|PubMed:21533550}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:21533550}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=H6TFZ4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=H6TFZ4-2; Sequence=VSP_044079;
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in roots, flowers and
CC       immature seeds. {ECO:0000269|PubMed:21533550}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC       synthase subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE03984.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; HQ895723; AEX32878.1; -; mRNA.
DR   EMBL; AL662942; CAE03984.3; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008210; BAF14346.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS88514.1; -; Genomic_DNA.
DR   EMBL; AK063304; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015634172.1; XM_015778686.1. [H6TFZ4-1]
DR   AlphaFoldDB; H6TFZ4; -.
DR   SMR; H6TFZ4; -.
DR   STRING; 4530.OS04T0309600-01; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   PaxDb; H6TFZ4; -.
DR   PRIDE; H6TFZ4; -.
DR   EnsemblPlants; Os04t0309600-01; Os04t0309600-01; Os04g0309600. [H6TFZ4-2]
DR   GeneID; 4335447; -.
DR   Gramene; Os04t0309600-01; Os04t0309600-01; Os04g0309600. [H6TFZ4-2]
DR   KEGG; osa:4335447; -.
DR   eggNOG; KOG0853; Eukaryota.
DR   HOGENOM; CLU_019158_1_0_1; -.
DR   InParanoid; H6TFZ4; -.
DR   OMA; LEPVDNT; -.
DR   OrthoDB; 115766at2759; -.
DR   BRENDA; 2.4.1.13; 4460.
DR   PlantReactome; R-OSA-1119452; Galactose degradation II.
DR   PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; H6TFZ4; OS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016157; F:sucrose synthase activity; IBA:GO_Central.
DR   GO; GO:0005985; P:sucrose metabolic process; IEA:InterPro.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR000368; Sucrose_synth.
DR   InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR   PANTHER; PTHR45839; PTHR45839; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF00862; Sucrose_synth; 1.
DR   TIGRFAMs; TIGR02470; sucr_synth; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasm; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase.
FT   CHAIN           1..855
FT                   /note="Sucrose synthase 5"
FT                   /id="PRO_0000418807"
FT   REGION          279..758
FT                   /note="GT-B glycosyltransferase"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         589..599
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_044079"
SQ   SEQUENCE   855 AA;  97812 MW;  4A0C5F64A0346B50 CRC64;
     MASKLSFKRM DSIAETMPDA LRQSRYQMKR CFQRYVSKGK RLLKNQQLME ELEKSLDDKV
     EKEKLVEGFL GYIICSTQEA VVLPPFVAFA VRMNPGIWEY VKVHSDDLSV EGITPSEYLK
     FKETLYDEKW AKDDNSLEVD FGALDLSTPH LTLPSSIGNG LQFVSKFMSS KLGGKPESMK
     PLLDYLLTLN YRGEKLMIND TIDTVSKLQT ALLLAEVFVS GLPKYTPYLK FEQRFQEWGL
     ERGWGDTAER CKETLNCLSE VLQAPDPTNM EKFFSRVPSI FNIVIFSIHG YFGQEKVLGL
     PDTGGQVVYI LDQVRAMEEE LLQRIKQQGL HVTPKILVLT RLIPDAKGTK CNVELEPVEN
     TKYSHILRVP FKTEDGKDLR QWVSRFDIYP YLERYAQDSC AKILDILEGK PDLIIGNYTD
     GNLVASLLSN KLCVTQGTIA HALEKTKYED SDVKWREMDQ KYHFSCQFTA DMISMNTSDF
     IITSTYQEIA GSKEKPGQYE HHYAFTMPGL CRYATGINVF DPKFNIAAPG ADQSIYFPFT
     QKQKRLTDLH PQIDELLYSK DDTDEHIGYL ADRNKPIIFS MARLDKVKNI TGLVEWYGQN
     KKLRDLVNLV VVAGLLDASQ SKDREEIEEI NKMHNLMDRY QLKGQIRWIK AQTDRVRNGE
     LYRCIADTKG AFVQPALYEA FGLTVIEAMN CGLPTFATNQ GGPAEIIIDG VSGFHVNPIN
     DREAGIKIAD FFQKCKEDPS YWNKVSTAGL QRICECYTWK IYATRVLNMG STYSFWKTLN
     KEERQAKQRY LQIFYNVQYR NLAKAMARAG DQQARQTTTG VAPSEIVVRP KERKPQTRMQ
     RILTRLAGQK PPVSE