SUT1_ORYSJ
ID SUT1_ORYSJ Reviewed; 538 AA.
AC Q10R54; A0A0P0VTV7; O49838; Q10R52; Q10R53; Q10R55; Q8S7X2;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Sucrose transport protein SUT1;
DE AltName: Full=Sucrose permease 1;
DE AltName: Full=Sucrose transporter 1;
DE Short=OsSUT1;
DE AltName: Full=Sucrose-proton symporter 1;
GN Name=SUT1; OrderedLocusNames=Os03g0170900, LOC_Os03g07480;
GN ORFNames=OSJNBa0091P11.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare; TISSUE=Leaf;
RX PubMed=9522469; DOI=10.1093/oxfordjournals.pcp.a029134;
RA Hirose T., Imaizumi N., Scofield G.N., Furbank R.T., Ohsugi R.;
RT "cDNA cloning and tissue specific expression of a gene for sucrose
RT transporter from rice (Oryza sativa L.).";
RL Plant Cell Physiol. 38:1389-1396(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=10982424; DOI=10.1104/pp.124.1.85;
RA Matsukura C., Saitoh T., Hirose T., Ohsugi R., Perata P., Yamaguchi J.;
RT "Sugar uptake and transport in rice embryo. Expression of companion cell-
RT specific sucrose transporter (OsSUT1) induced by sugar and light.";
RL Plant Physiol. 124:85-93(2000).
RN [8]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12668768; DOI=10.1093/pcp/pcg030;
RA Aoki N., Hirose T., Scofield G.N., Whitfeld P.R., Furbank R.T.;
RT "The sucrose transporter gene family in rice.";
RL Plant Cell Physiol. 44:223-232(2003).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20603282; DOI=10.1093/jxb/erq175;
RA Hirose T., Zhang Z., Miyao A., Hirochika H., Ohsugi R., Terao T.;
RT "Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen
RT function but pollen maturation is unaffected.";
RL J. Exp. Bot. 61:3639-3646(2010).
RN [10]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=19965875; DOI=10.1093/pcp/pcp172;
RA Sun Y., Reinders A., LaFleur K.R., Mori T., Ward J.M.;
RT "Transport activity of rice sucrose transporters OsSUT1 and OsSUT5.";
RL Plant Cell Physiol. 51:114-122(2010).
CC -!- FUNCTION: Responsible for the transport of sucrose into the cell, with
CC the concomitant uptake of protons (symport system). Can also transport
CC other glucosides such as maltose, salicin, helicin, and alpha-
CC phenylglucoside. Probably required for apoplastic phloem sucrose
CC loading in source tissues (e.g. leaves) in order to transport it to
CC sink tissues (e.g. roots, flowers). Probably not involved in transport
CC of sugars across the symplastic discontinuity between the endosperm and
CC the embryo. Essential for normal pollen germination, but not for pollen
CC maturation or starch accumulation in pollen.
CC {ECO:0000269|PubMed:19965875, ECO:0000269|PubMed:20603282,
CC ECO:0000269|PubMed:9522469}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=7.5 mM for sucrose (at pH 5.6) {ECO:0000269|PubMed:19965875};
CC pH dependence:
CC Optimum pH is 5. {ECO:0000269|PubMed:19965875};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q10R54-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q10R54-2; Sequence=VSP_039741;
CC Name=3;
CC IsoId=Q10R54-3; Sequence=VSP_039742, VSP_039743;
CC Name=4;
CC IsoId=Q10R54-4; Sequence=VSP_039739, VSP_039740;
CC -!- TISSUE SPECIFICITY: Widely expressed. Highly expressed in embryo,
CC endosperm, germinating seeds, source leaf sheaths and panicles, but at
CC very low levels in roots. Expressed in phloem companion cells.
CC {ECO:0000269|PubMed:10982424, ECO:0000269|PubMed:12668768,
CC ECO:0000269|PubMed:9522469}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing caryopses from 3 days
CC after flowering (DAF), increases to a maximal level at 5 to 7 DAF, and
CC then gradually declines to a barely detectable level by 20 DAF when
CC grain filling stage nearly terminates. Expressed in developing pollen
CC and anther walls from 5 to 1 day before anthesis.
CC {ECO:0000269|PubMed:12668768, ECO:0000269|PubMed:20603282}.
CC -!- INDUCTION: By exogenous sugar supply. Down-regulated by sugar
CC starvation and light in dark-grown seedlings.
CC {ECO:0000269|PubMed:10982424, ECO:0000269|PubMed:9522469}.
CC -!- DISRUPTION PHENOTYPE: Male sterility probably due to impaired pollen
CC germination. {ECO:0000269|PubMed:20603282}.
CC -!- SIMILARITY: Belongs to the glycoside-pentoside-hexuronide (GPH) cation
CC symporter transporter (TC 2.A.2.4) family. {ECO:0000305}.
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DR EMBL; D87819; BAA24071.1; -; mRNA.
DR EMBL; AC073556; AAL84308.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94211.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94212.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94213.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94214.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11025.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82530.1; -; Genomic_DNA.
DR EMBL; AK100027; BAG94404.1; -; mRNA.
DR PIR; T02982; T02982.
DR RefSeq; XP_015633173.1; XM_015777687.1. [Q10R54-2]
DR RefSeq; XP_015633174.1; XM_015777688.1. [Q10R54-1]
DR AlphaFoldDB; Q10R54; -.
DR STRING; 4530.OS03T0170900-01; -.
DR PaxDb; Q10R54; -.
DR PRIDE; Q10R54; -.
DR EnsemblPlants; Os03t0170900-01; Os03t0170900-01; Os03g0170900. [Q10R54-1]
DR GeneID; 4331775; -.
DR Gramene; Os03t0170900-01; Os03t0170900-01; Os03g0170900. [Q10R54-1]
DR KEGG; osa:4331775; -.
DR eggNOG; KOG0637; Eukaryota.
DR HOGENOM; CLU_025234_3_0_1; -.
DR InParanoid; Q10R54; -.
DR OMA; FQWYALF; -.
DR OrthoDB; 1230185at2759; -.
DR UniPathway; UPA00238; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q10R54; baseline and differential.
DR Genevisible; Q10R54; OS.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005364; F:maltose:proton symporter activity; IDA:UniProtKB.
DR GO; GO:0042950; F:salicin transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008506; F:sucrose:proton symporter activity; IDA:UniProtKB.
DR GO; GO:0015768; P:maltose transport; IDA:UniProtKB.
DR GO; GO:0009846; P:pollen germination; IMP:UniProtKB.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015770; P:sucrose transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005989; Suc_symporter_pln.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR01301; GPH_sucrose; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Sugar transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..538
FT /note="Sucrose transport protein SUT1"
FT /id="PRO_0000398189"
FT TOPO_DOM 1..52
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..162
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..247
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..306
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..357
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..388
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 410..433
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..470
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 492..499
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 500..520
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 521..538
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 447..453
FT /note="LYSVPFA -> RITDTST (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_039739"
FT VAR_SEQ 454..538
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_039740"
FT VAR_SEQ 468..538
FT /note="LCTGVLNISIVIPQVVIALGAGPWDELFGKGNIPAFGLASGFALIGGVAGIF
FT LLPKISKRQFRSVSMGGGH -> MNPNILAAFLDIIRCCLDAGLIHGDLQGSAPASSTS
FT PSSSLRWSSRWEPGRGTSCSGRGTSRRSASPRDSRSSAASPEYSCCPRSPSASSGRSAW
FT AAVTDRPALAAGCGGGGGRSSAAGSGELCSSRLQWIKGAPSFFLHRVIN (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_039741"
FT VAR_SEQ 483
FT /note="V -> N (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_039742"
FT VAR_SEQ 484..538
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_039743"
FT CONFLICT 29
FT /note="Missing (in Ref. 1; BAA24071)"
FT /evidence="ECO:0000305"
FT CONFLICT 530
FT /note="R -> W (in Ref. 1; BAA24071)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 56158 MW; 8DC0E7712A2167A1 CRC64;
MARGSGAGGG GGGGGGGLEL SVGVGGGGGA RGGGGGEAAA AVETAAPISL GRLILSGMVA
GGVQYGWALQ LSLLTPYVQT LGLSHALTSF MWLCGPIAGM VVQPCVGLYS DRCTSKWGRR
RPYILTGCVL ICLAVVVIGF SADIGYAMGD TKEDCSVYHG SRWHAAIVYV LGFWLLDFSN
NTVQGPARAL MADLSGRHGP GTANSIFCSW MAMGNILGYS SGSTNNWHKW FPFLKTRACC
EACANLKGAF LVAVIFLSLC LVITLIFAKE VPFKGNAALP TKSNEPAEPE GTGPLAVLKG
FRNLPTGMPS VLIVTGLTWL SWFPFILYDT DWMGREIYHG DPKGTDPQIE AFNQGVRAGA
FGLLLNSIVL GFSSFLIEPM CRKVGPRVVW VTSNFLVCIA MAATALISFW SLKDFHGTVQ
KAITADKSIK AVCLVLFAFL GVPLAVLYSV PFAVTAQLAA TRGGGQGLCT GVLNISIVIP
QVVIALGAGP WDELFGKGNI PAFGLASGFA LIGGVAGIFL LPKISKRQFR SVSMGGGH
