SUT1_YEAST
ID SUT1_YEAST Reviewed; 299 AA.
AC P53032; D6VTY9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Sterol uptake protein 1;
GN Name=SUT1; OrderedLocusNames=YGL162W; ORFNames=G1828;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX PubMed=7489925; DOI=10.1016/0378-1119(95)00478-o;
RA Bourot S., Karst F.;
RT "Isolation and characterization of the Saccharomyces cerevisiae SUT1 gene
RT involved in sterol uptake.";
RL Gene 165:97-102(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8585324; DOI=10.1002/yea.320111409;
RA James C.M., Indge K.J., Oliver S.G.;
RT "DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae
RT chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2,
RT PMR1, RCK1, AMS1 and CAL1/CDC43.";
RL Yeast 11:1413-1419(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP FUNCTION.
RX PubMed=11248676; DOI=10.1046/j.1432-1033.2001.02029.x;
RA Ness F., Bourot S., Regnacq M., Spagnoli R., Berges T., Karst F.;
RT "SUT1 is a putative Zn[II]2Cys6-transcription factor whose upregulation
RT enhances both sterol uptake and synthesis in aerobically growing
RT Saccharomyces cerevisiae cells.";
RL Eur. J. Biochem. 268:1585-1595(2001).
RN [6]
RP FUNCTION, AND INTERACTION WITH SSN6.
RX PubMed=11401714; DOI=10.1046/j.1365-2958.2001.02450.x;
RA Regnacq M., Alimardani P., El Moudni B., Berges T.;
RT "SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-
RT Tup1p repression in Saccharomyces cerevisiae.";
RL Mol. Microbiol. 40:1085-1096(2001).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Transcription factor involved in the induction of hypoxic
CC gene transcription when the cells are shifted from aerobiosis to
CC anaerobiosis. Involved in sterol uptake. {ECO:0000269|PubMed:11248676,
CC ECO:0000269|PubMed:11401714}.
CC -!- SUBUNIT: Interacts with SSN6/CYC8. {ECO:0000269|PubMed:11401714}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1100 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SUT1 family. {ECO:0000305}.
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DR EMBL; X77766; CAA54806.1; -; Genomic_DNA.
DR EMBL; Z48618; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z72684; CAA96874.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07950.1; -; Genomic_DNA.
DR PIR; JC4374; JC4374.
DR RefSeq; NP_011353.1; NM_001181027.1.
DR AlphaFoldDB; P53032; -.
DR BioGRID; 33091; 98.
DR DIP; DIP-5748N; -.
DR IntAct; P53032; 10.
DR MINT; P53032; -.
DR STRING; 4932.YGL162W; -.
DR iPTMnet; P53032; -.
DR MaxQB; P53032; -.
DR PaxDb; P53032; -.
DR PRIDE; P53032; -.
DR EnsemblFungi; YGL162W_mRNA; YGL162W; YGL162W.
DR GeneID; 852714; -.
DR KEGG; sce:YGL162W; -.
DR SGD; S000003130; SUT1.
DR VEuPathDB; FungiDB:YGL162W; -.
DR eggNOG; ENOG502S235; Eukaryota.
DR GeneTree; ENSGT00940000176592; -.
DR HOGENOM; CLU_051216_0_0_1; -.
DR InParanoid; P53032; -.
DR OMA; ADSNECA; -.
DR BioCyc; YEAST:G3O-30651-MON; -.
DR PRO; PR:P53032; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53032; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IGI:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR CDD; cd00067; GAL4; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR SUPFAM; SSF57701; SSF57701; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..299
FT /note="Sterol uptake protein 1"
FT /id="PRO_0000115014"
FT REGION 133..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 299 AA; 33191 MW; A77B0B506177586D CRC64;
MSTSITVRNR DRSLPPLLLP NVSLLEKDIR RKGTQNVGIT DPELLSTTWT RKRAFPTDEL
LGGYKRLKPA AADSNECAIG IATVTPPPTL PVSAIVPPPQ NYTPPLFEYH PHALASMVNE
DANASCTQMS IISRSTSNST TSSATSTSSI SKRQRSGPSC DKCRLKKIKC NAKIEILLQD
DTIMPMISNK LRYVLTPDDI RLYRGTLLRN IAIPDDVIEG TGSRKLIKHI DKLVLLTPCL
PCIKKKHSSS STNFPKNDKC TFSKGFTRAD INISSKISLK FKDKTIYDIT YDDYKSIDF
