SUT21_ARATH
ID SUT21_ARATH Reviewed; 677 AA.
AC O04722;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 146.
DE RecName: Full=Sulfate transporter 2.1;
DE AltName: Full=AST68;
GN Name=SULTR2;1; OrderedLocusNames=At5g10180; ORFNames=T31P16_170;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=9380766; DOI=10.1073/pnas.94.20.11102;
RA Takahashi H., Yamazaki M., Sasakura N., Watanabe A., Leustek T.,
RA de Almeida Engler J., Engler G., Van Montagu M., Saito K.;
RT "Regulation of sulfur assimilation in higher plants: a sulfate transporter
RT induced in sulfate-starved roots plays a central role in Arabidopsis
RT thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:11102-11107(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=10929111; DOI=10.1046/j.1365-313x.2000.00768.x;
RA Takahashi H., Watanabe-Takahashi A., Smith F.W., Blake-Kalff M.,
RA Hawkesford M.J., Saito K.;
RT "The roles of three functional sulphate transporters involved in uptake and
RT translocation of sulphate in Arabidopsis thaliana.";
RL Plant J. 23:171-182(2000).
CC -!- FUNCTION: Low-affinity H(+)/sulfate cotransporter that may be involved
CC in root-to-shoot translocation of sulfate. Plays a central role in the
CC regulation of sulfate assimilation. {ECO:0000269|PubMed:10929111}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in root cap, central cylinder of roots
CC and in vascular tissues of leaves. {ECO:0000269|PubMed:10929111,
CC ECO:0000269|PubMed:9380766}.
CC -!- INDUCTION: In roots by sulfate starvation or after selenate treatment.
CC {ECO:0000269|PubMed:10929111, ECO:0000269|PubMed:9380766}.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
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DR EMBL; AB003590; BAA20084.1; -; Genomic_DNA.
DR EMBL; AB003591; BAA20085.1; -; mRNA.
DR EMBL; AL356332; CAB92059.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91505.1; -; Genomic_DNA.
DR EMBL; AY062546; AAL32624.1; -; mRNA.
DR EMBL; AY093335; AAM13334.1; -; mRNA.
DR PIR; T50022; T50022.
DR RefSeq; NP_196580.1; NM_121056.3.
DR AlphaFoldDB; O04722; -.
DR SMR; O04722; -.
DR BioGRID; 16160; 2.
DR IntAct; O04722; 1.
DR STRING; 3702.AT5G10180.1; -.
DR PaxDb; O04722; -.
DR PRIDE; O04722; -.
DR ProteomicsDB; 226829; -.
DR EnsemblPlants; AT5G10180.1; AT5G10180.1; AT5G10180.
DR GeneID; 830882; -.
DR Gramene; AT5G10180.1; AT5G10180.1; AT5G10180.
DR KEGG; ath:AT5G10180; -.
DR Araport; AT5G10180; -.
DR TAIR; locus:2184158; AT5G10180.
DR eggNOG; KOG0236; Eukaryota.
DR HOGENOM; CLU_003182_13_2_1; -.
DR InParanoid; O04722; -.
DR OMA; MTVGLFQ; -.
DR OrthoDB; 289441at2759; -.
DR PhylomeDB; O04722; -.
DR PRO; PR:O04722; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O04722; baseline and differential.
DR Genevisible; O04722; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015301; F:anion:anion antiporter activity; IBA:GO_Central.
DR GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:1902358; P:sulfate transmembrane transport; IGI:TAIR.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR018045; S04_transporter_CS.
DR InterPro; IPR011547; SLC26A/SulP_dom.
DR InterPro; IPR001902; SLC26A/SulP_fam.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR PANTHER; PTHR11814; PTHR11814; 1.
DR Pfam; PF01740; STAS; 1.
DR Pfam; PF00916; Sulfate_transp; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR TIGRFAMs; TIGR00815; sulP; 1.
DR PROSITE; PS01130; SLC26A; 1.
DR PROSITE; PS50801; STAS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Sulfate transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..677
FT /note="Sulfate transporter 2.1"
FT /id="PRO_0000080175"
FT TOPO_DOM 1..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..360
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..426
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..490
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 491..511
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 512..677
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 548..672
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT REGION 23..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 255
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 677 AA; 74068 MW; EDFA66FB7E9FBE89 CRC64;
MKERDSESFE SLSHQVLPNT SNSTHMIQMA MANSGSSAAA QAGQDQPDRS KWLLDCPEPP
SPWHELKRQV KGSFLTKAKK FKSLQKQPFP KQILSVLQAI FPIFGWCRNY KLTMFKNDLM
AGLTLASLCI PQSIGYATLA KLDPQYGLYT SVVPPLIYAL MGTSREIAIG PVAVVSLLIS
SMLQKLIDPE TDPLGYKKLV LTTTFFAGIF QASFGLFRLG FLVDFLSHAA IVGFMGGAAI
VIGLQQLKGL LGITNFTTNT DIVSVLRAVW RSCQQQWSPH TFILGCSFLS FILITRFIGK
KYKKLFWLPA IAPLIAVVVS TLMVFLTKAD EHGVKTVRHI KGGLNPMSIQ DLDFNTPHLG
QIAKIGLIIA IVALTEAIAV GRSFAGIKGY RLDGNKEMVA IGFMNVLGSF TSCYAATGSF
SRTAVNFAAG CETAMSNIVM AVTVFVALEC LTRLLYYTPI AILASIILSA LPGLININEA
IHIWKVDKFD FLALIGAFFG VLFASVEIGL LVAVVISFAK IILISIRPGI ETLGRMPGTD
TFTDTNQYPM TVKTPGVLIF RVKSALLCFA NASSIEERIM GWVDEEEEEE NTKSNAKRKI
LFVVLDMSSL INVDTSGITA LLELHNKLIK TGVELVIVNP KWQVIHKLNQ AKFVDRIGGK
VYLTIGEALD ACFGLKV
