ID   SUT2_STYHA              Reviewed;         662 AA.
AC   P53392;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=High affinity sulfate transporter 2;
GN   Name=ST2;
OS   Stylosanthes hamata (Caribbean stylo).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   dalbergioids sensu lato; Dalbergieae; Pterocarpus clade; Stylosanthes.
OX   NCBI_TaxID=37660;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Verano; TISSUE=Root;
RX   PubMed=7568135; DOI=10.1073/pnas.92.20.9373;
RA   Smith F.W., Ealing P.M., Hawkesford M.J., Clarkson D.T.;
RT   "Plant members of a family of sulfate transporters reveal functional
RT   subtypes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:9373-9377(1995).
CC   -!- FUNCTION: High-affinity H(+)/sulfate cotransporter that mediates the
CC       uptake of sulfate by plant roots from low concentrations of sulfate in
CC       the soil solution.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC       {ECO:0000305}.
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DR   EMBL; X82256; CAA57711.1; -; mRNA.
DR   PIR; S51764; S51764.
DR   AlphaFoldDB; P53392; -.
DR   SMR; P53392; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 3.30.750.24; -; 1.
DR   InterPro; IPR018045; S04_transporter_CS.
DR   InterPro; IPR011547; SLC26A/SulP_dom.
DR   InterPro; IPR001902; SLC26A/SulP_fam.
DR   InterPro; IPR002645; STAS_dom.
DR   InterPro; IPR036513; STAS_dom_sf.
DR   PANTHER; PTHR11814; PTHR11814; 1.
DR   Pfam; PF01740; STAS; 1.
DR   Pfam; PF00916; Sulfate_transp; 1.
DR   SUPFAM; SSF52091; SSF52091; 1.
DR   TIGRFAMs; TIGR00815; sulP; 1.
DR   PROSITE; PS01130; SLC26A; 1.
DR   PROSITE; PS50801; STAS; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..662
FT                   /note="High affinity sulfate transporter 2"
FT                   /id="PRO_0000080190"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        383..403
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        420..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        447..467
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        481..501
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          532..655
FT                   /note="STAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..28
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   662 AA;  72728 MW;  7544D1E73FB76284 CRC64;
     MSQRVSDQAM AEVIAETRTN SSSRRHGGGD DTPSLPYMHK VGAPPKQTLF QEIKHSFNET
     FFPDKPFGNF KDQSGSRKFV LGLQYIFPIL EWGRHYDLKK FRGDFIAGLT IASLCIPQDL
     AYAKLANLDP WYGLYSSFVA PLVYAFMGTS RDIAIGPVAV VSLLLGTLLS NEISNTKSHD
     YLRLAFTATF FAGVTQMLLG VCRLGFLIDF LSHAAIVGFM AGAAITIGLQ QLKGLLGIKD
     FTKNSDIVSV MHSVWSNVHH GWNWETILIG LSFLIFLLIT KYIAKKNKKL FWVSAISPMI
     CVIVSTFFVY ITRADKRGVT IVKHIKSGVN PSSANEIFFH GKYLGAGVRV GVVAGLVALT
     EAMAIGRTFA AMKDYSIDGN KEMVAMGTMN IVGSLTSCYV TTGSFSRSAV NYMAGCKTAV
     SNIVMAIVVL LTLLVITPLF KYTPNAVLAS IIIAAVVNLV NIEAMVLLWK IDKFDFVACM
     GAFFGVIFKS VEIGLLIAVA ISFAKILLQV TRPRTAVLGK LPGTSVYRNI QQYPKAEQIP
     GMLIIRVDSA IYFSNSNYIK ERILRWLIDE GAQRTESELP EIQHLIVEMS PVTDIDTSGI
     HAFEELYKTL QKREVQLMLA NPGPVVIEKL HASNLAELIG EDKIFLTVAD AVATYGPKTA
     AF