SUT5_ORYSJ
ID SUT5_ORYSJ Reviewed; 535 AA.
AC Q69JW3; A0A0N7KFJ4; Q4PKJ2; Q84KR3;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Sucrose transport protein SUT5;
DE AltName: Full=Sucrose permease 5;
DE AltName: Full=Sucrose transporter 5;
DE Short=OsSUT5;
DE AltName: Full=Sucrose-proton symporter 5;
GN Name=SUT5; Synonyms=SUT5Z; OrderedLocusNames=Os02g0576600, LOC_Os02g36700;
GN ORFNames=B1342F01.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Nipponbare; TISSUE=Panicle;
RX PubMed=12668768; DOI=10.1093/pcp/pcg030;
RA Aoki N., Hirose T., Scofield G.N., Whitfeld P.R., Furbank R.T.;
RT "The sucrose transporter gene family in rice.";
RL Plant Cell Physiol. 44:223-232(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Zhonghua 11; TISSUE=Grain;
RX PubMed=18666953; DOI=10.1111/j.1744-7909.2007.00596.x;
RA Sun A.J., Xu H.L., Gong W.K., Zhai H.L., Meng K., Wang Y.Q., Wei X.L.,
RA Xiao G.F., Zhu Z.;
RT "Cloning and expression analysis of rice sucrose transporter genes OsSUT2M
RT and OsSUT5Z.";
RL J. Integr. Plant Biol. 50:62-75(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=19965875; DOI=10.1093/pcp/pcp172;
RA Sun Y., Reinders A., LaFleur K.R., Mori T., Ward J.M.;
RT "Transport activity of rice sucrose transporters OsSUT1 and OsSUT5.";
RL Plant Cell Physiol. 51:114-122(2010).
CC -!- FUNCTION: Responsible for the transport of sucrose into the cell, with
CC the concomitant uptake of protons (symport system). Can also transport
CC other glucosides such as maltose, arbutin, salicin, helicin, alpha-
CC phenylglucoside and beta-phenylglucoside.
CC {ECO:0000269|PubMed:19965875}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.32 mM for sucrose (at pH 5.6) {ECO:0000269|PubMed:19965875};
CC pH dependence:
CC Optimum pH is 5. {ECO:0000269|PubMed:19965875};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Highest expression in sink leaves
CC and lowest in germinating seeds. {ECO:0000269|PubMed:12668768,
CC ECO:0000269|PubMed:18666953}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing caryopses from 1 to 5 days
CC after flowering (DAF) and then declines to nearly undetectable levels
CC by 15 DAF. {ECO:0000269|PubMed:12668768, ECO:0000269|PubMed:18666953}.
CC -!- SIMILARITY: Belongs to the glycoside-pentoside-hexuronide (GPH) cation
CC symporter transporter (TC 2.A.2.4) family. {ECO:0000305}.
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DR EMBL; AB091674; BAC67165.1; -; mRNA.
DR EMBL; DQ072593; AAY83289.1; -; mRNA.
DR EMBL; AP006070; BAD34249.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09131.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79385.1; -; Genomic_DNA.
DR EMBL; AK073105; BAG93290.1; -; mRNA.
DR RefSeq; XP_015624726.1; XM_015769240.1.
DR AlphaFoldDB; Q69JW3; -.
DR STRING; 4530.OS02T0576600-01; -.
DR TCDB; 2.A.2.4.11; the glycoside-pentoside-hexuronide (gph):cation symporter family.
DR PaxDb; Q69JW3; -.
DR PRIDE; Q69JW3; -.
DR EnsemblPlants; Os02t0576600-01; Os02t0576600-01; Os02g0576600.
DR GeneID; 4329756; -.
DR Gramene; Os02t0576600-01; Os02t0576600-01; Os02g0576600.
DR KEGG; osa:4329756; -.
DR eggNOG; KOG0637; Eukaryota.
DR HOGENOM; CLU_025234_3_0_1; -.
DR InParanoid; Q69JW3; -.
DR OMA; VSMRGYR; -.
DR OrthoDB; 1230185at2759; -.
DR UniPathway; UPA00238; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q69JW3; OS.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0042951; F:arbutin transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005364; F:maltose:proton symporter activity; IDA:UniProtKB.
DR GO; GO:0042950; F:salicin transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008506; F:sucrose:proton symporter activity; IDA:UniProtKB.
DR GO; GO:0015768; P:maltose transport; IDA:UniProtKB.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015770; P:sucrose transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005989; Suc_symporter_pln.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR01301; GPH_sucrose; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..535
FT /note="Sucrose transport protein SUT5"
FT /id="PRO_0000398195"
FT TOPO_DOM 1..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..87
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..249
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..354
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..384
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..429
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..498
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 520..535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 26
FT /note="T -> M (in Ref. 1; BAC67165)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="Q -> R (in Ref. 2; AAY83289)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="V -> A (in Ref. 2; AAY83289)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="F -> Y (in Ref. 2; AAY83289)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="N -> D (in Ref. 1; BAC67165)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="E -> K (in Ref. 2; AAY83289)"
FT /evidence="ECO:0000305"
FT CONFLICT 418
FT /note="A -> T (in Ref. 2; AAY83289)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 535 AA; 56979 MW; 19F0FC13C6276FB0 CRC64;
MEEGRRGDRE GKSAAGWTAL STTKTTLEEK RRLQANGSVG GDAGTSGFRR IVRLFFACMV
AGGIQYGWAL QLSLLSPYSQ TLGISHSYVS LTWICGPIAG FVVQPIVGYY SDRCTMKMGR
RRPFILVGCL IICISVMIIG FSADIGRHLG DTKEHCSTYT GPRWSAAMVY IVGFWFLDFA
NNTVQGPARA MMADLSAGHH GPNVGQSIFS LWMAIGSVLG YLSGANGKWH EWFPWLKTAA
CCDACANLKG AFFTAVLLIV VSMTVTMYLA DEMPLDKQDV DTSGGGGCAV FVDLFKSLRN
LPPAMFKVLA VTAVTWLSWF PFIQYNTDWM GREIYHGEPQ GTAAKADVYD AGVREGAMGL
LFCSVALGVT SFVIPKLCRR LTSKVVWSIS NFLVFALMAV MVAVGMVSMR GYRPSLAAGL
TGPDPTLKAV ALVVFALIGI PQAVLFSVPW AVASEVTAEE GGGQGLAIGV LNIAIVVPQL
VIALTAGPID GAFNKGNTPA FGIGGAFAFI CGVLALIWLP KTRGVSNAAV VAGGH
