SWA1_ARATH
ID SWA1_ARATH Reviewed; 530 AA.
AC O82266; Q8GXN5;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein SLOW WALKER 1 {ECO:0000303|PubMed:15980260};
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 13 {ECO:0000303|PubMed:15634699};
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 19 {ECO:0000303|PubMed:15634699};
GN Name=SWA1 {ECO:0000303|PubMed:15980260};
GN Synonyms=EDA13 {ECO:0000303|PubMed:15634699},
GN EDA19 {ECO:0000303|PubMed:15634699};
GN OrderedLocusNames=At2g47990 {ECO:0000312|Araport:AT2G47990};
GN ORFNames=F17A22 {ECO:0000312|EMBL:AAC63653.1},
GN T9J23.30 {ECO:0000312|EMBL:AAM15129.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=15980260; DOI=10.1105/tpc.105.033563;
RA Shi D.-Q., Liu J., Xiang Y.-H., Ye D., Sundaresan V., Yang W.-C.;
RT "SLOW WALKER1, essential for gametogenesis in Arabidopsis, encodes a WD40
RT protein involved in 18S ribosomal RNA biogenesis.";
RL Plant Cell 17:2340-2354(2005).
CC -!- FUNCTION: Essential protein required for nuclear division and
CC organization during embryo sac development in female gametophyte,
CC probably by promoting rRNA biogenesis essential for the progression of
CC the mitotic division cycles during gametogenesis (PubMed:15634699,
CC PubMed:15980260). Involved in nucleolar processing of pre-18S ribosomal
CC RNA (PubMed:15980260). {ECO:0000269|PubMed:15634699,
CC ECO:0000269|PubMed:15980260}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:15980260}.
CC Note=Localized in the nucleolus in interphase cells.
CC {ECO:0000269|PubMed:15980260}.
CC -!- TISSUE SPECIFICITY: Expressed in cells undergoing active cell
CC divisions, including functional megaspores and the female gametophytic
CC cells. Accumulates in roots, stems, leaves, inflorescences and
CC siliques. {ECO:0000269|PubMed:15980260}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in root tips and lateral root
CC primordia. Present in young leaf and stem vascular tissues. Expressed
CC throughout pollen development from very young floral buds to dehisced
CC anthers, especially in microsporogenous cells, microspores, and mature
CC pollen grains. In female reproductive organs, detected in megaspores
CC and embryo sacs from one-nucleate to seven-celled embryo sacs.
CC {ECO:0000269|PubMed:15980260}.
CC -!- DISRUPTION PHENOTYPE: Embryo sac development arrest at two-, four-, or
CC eight-nucleate stages, associated with abnormal nuclear numbers and
CC positions in embryo sac, aberrant embryo sacs and unfused polar nuclei
CC (PubMed:15634699). Disrupted progression of the mitotic division cycles
CC of the female gametophyte leading to an impaired synchrony of female
CC gametophyte development (PubMed:15980260). Reduced root growth and
CC accumulation of unprocessed 18S pre-rRNA (PubMed:15980260).
CC {ECO:0000269|PubMed:15634699, ECO:0000269|PubMed:15980260}.
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DR EMBL; AC005309; AAC63653.1; -; Genomic_DNA.
DR EMBL; AC006072; AAM15129.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10921.1; -; Genomic_DNA.
DR EMBL; AK118137; BAC42762.1; -; mRNA.
DR PIR; A84922; A84922.
DR RefSeq; NP_182320.1; NM_130366.3.
DR AlphaFoldDB; O82266; -.
DR SMR; O82266; -.
DR STRING; 3702.AT2G47990.1; -.
DR PaxDb; O82266; -.
DR PRIDE; O82266; -.
DR ProteomicsDB; 226789; -.
DR DNASU; 819411; -.
DR EnsemblPlants; AT2G47990.1; AT2G47990.1; AT2G47990.
DR GeneID; 819411; -.
DR Gramene; AT2G47990.1; AT2G47990.1; AT2G47990.
DR KEGG; ath:AT2G47990; -.
DR Araport; AT2G47990; -.
DR TAIR; locus:2043333; AT2G47990.
DR eggNOG; KOG0310; Eukaryota.
DR HOGENOM; CLU_021102_4_0_1; -.
DR InParanoid; O82266; -.
DR OMA; WKEHKQP; -.
DR OrthoDB; 621827at2759; -.
DR PhylomeDB; O82266; -.
DR PRO; PR:O82266; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82266; baseline and differential.
DR Genevisible; O82266; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0009561; P:megagametogenesis; IMP:TAIR.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR018983; U3_snoRNA-assocProt_15_C.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF09384; UTP15_C; 1.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Developmental protein; Nucleus;
KW Reference proteome; Repeat; rRNA processing; WD repeat.
FT CHAIN 1..530
FT /note="Protein SLOW WALKER 1"
FT /id="PRO_0000432652"
FT REPEAT 50..89
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 91..130
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 133..173
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 176..216
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 220..258
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 262..304
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 320..363
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT MOTIF 392..399
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT CONFLICT 188
FT /note="P -> S (in Ref. 3; BAC42762)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="K -> E (in Ref. 3; BAC42762)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 530 AA; 58903 MW; 6F1B4AB984F3162B CRC64;
MEEELRVRLN DHQVSKVFPV KPKSTAKPVS ESETPESRYW SSFKNHSTPN LVSSVAALAF
SPVHPHSLAV AHSATVSLFS SQSLSSSRRF SFRDVVSSVC FRSDGALFAA CDLSGVVQVF
DIKERMALRT LRSHSAPARF VKYPVQDKLH LVSGGDDGVV KYWDVAGATV ISDLLGHKDY
VRCGDCSPVN DSMLVTGSYD HTVKVWDARV HTSNWIAEIN HGLPVEDVVY LPSGGLIATA
GGNSVKVWDL IGGGKMVCSM ESHNKTVTSL RVARMESAES RLVSVALDGY MKVFDYGRAK
VTYSMRFPAP LMSLGLSPDG STRVIGGSNG MVFAGKKKVR DVVGGQKKSL NLWSLISDVD
ESRRRALRPT YFRYFQRGQS EKPSKDDYLV KEKKGLKLTR HDKLLKKFRH KEALVSVLEE
KKPANVVAVM EELVARRKLM KCVSNMEEGE LGMLLGFLQR YCTVQRYSGL LMGLTKKVLE
TRAEDIKGKN EFKGLLRNLK REVNQEIRIQ QSLLEIQGVI APLMRIAGRS
