SWA2_ARATH
ID SWA2_ARATH Reviewed; 1043 AA.
AC F4IDC2; B3TIK7; Q9C9E2;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Protein SLOW WALKER 2 {ECO:0000303|PubMed:19734265};
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 25 {ECO:0000303|PubMed:15634699};
GN Name=SWA2 {ECO:0000303|PubMed:19734265};
GN Synonyms=EDA25 {ECO:0000303|PubMed:15634699};
GN OrderedLocusNames=At1g72440 {ECO:0000312|Araport:AT1G72440};
GN ORFNames=T10D10.9 {ECO:0000312|EMBL:AAG52578.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, INTERACTION WITH NOC2, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=19734265; DOI=10.1104/pp.109.142414;
RA Li N., Yuan L., Liu N., Shi D., Li X., Tang Z., Liu J., Sundaresan V.,
RA Yang W.-C.;
RT "SLOW WALKER2, a NOC1/MAK21 homologue, is essential for coordinated cell
RT cycle progression during female gametophyte development in Arabidopsis.";
RL Plant Physiol. 151:1486-1497(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [5]
RP INTERACTION WITH RBL AND NOC2.
RX PubMed=30338215; DOI=10.1002/2211-5463.12504;
RA de Bossoreille S., Morel P., Trehin C., Negrutiu I.;
RT "REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana,
RT interacts with both nucleolar and nucleoplasmic proteins.";
RL FEBS Open Bio 8:1636-1648(2018).
CC -!- FUNCTION: Together with NOC2, probably involved in pre-ribosome export
CC from the nucleus to the cytoplasm (PubMed:19734265). Required for
CC coordinated cell cycle progression during female gametophyte and pollen
CC development (PubMed:19734265, PubMed:15634699).
CC {ECO:0000269|PubMed:15634699, ECO:0000269|PubMed:19734265}.
CC -!- SUBUNIT: Interacts with RBL in both the nucleolus and nucleoplasm
CC (PubMed:30338215). Binds to NOC2 (PubMed:19734265, PubMed:30338215).
CC {ECO:0000269|PubMed:19734265, ECO:0000269|PubMed:30338215}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC Nucleus, nucleolus {ECO:0000269|PubMed:19734265}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in actively dividing tissues (e.g.
CC root tips, lateral root primordia, shoot apices, young leaves,
CC inflorescences and pollen grains) through the plant, including roots,
CC stems, leaves, inflorescences, siliques and seedlings, and in
CC gametophytes. {ECO:0000269|PubMed:19734265}.
CC -!- DEVELOPMENTAL STAGE: During female gametophyte development, detected in
CC the gametophytic nucleus from one-nucleate to two-nucleate stages, and
CC fades out progressively during ovule maturation to be confined to the
CC central cell of mature embryo sac. {ECO:0000269|PubMed:19734265}.
CC -!- DISRUPTION PHENOTYPE: Female gametophyte mutant with impaired
CC progression of the mitotic cycles and lost synchrony of female
CC gametophyte development leading to an arrest of female gametophytes at
CC the two-, four-, or eight-nucleate stage and characterized by unfused
CC polar nuclei (PubMed:19734265, PubMed:15634699). Defective pollen
CC development (PubMed:19734265). {ECO:0000269|PubMed:15634699,
CC ECO:0000269|PubMed:19734265}.
CC -!- SIMILARITY: Belongs to the CBF/MAK21 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52578.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EU170440; ABX24524.1; -; mRNA.
DR EMBL; AC016529; AAG52578.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35325.1; -; Genomic_DNA.
DR PIR; E96748; E96748.
DR RefSeq; NP_177388.2; NM_105903.4.
DR AlphaFoldDB; F4IDC2; -.
DR STRING; 3702.AT1G72440.1; -.
DR iPTMnet; F4IDC2; -.
DR PaxDb; F4IDC2; -.
DR PRIDE; F4IDC2; -.
DR ProteomicsDB; 197460; -.
DR EnsemblPlants; AT1G72440.1; AT1G72440.1; AT1G72440.
DR GeneID; 843576; -.
DR Gramene; AT1G72440.1; AT1G72440.1; AT1G72440.
DR KEGG; ath:AT1G72440; -.
DR Araport; AT1G72440; -.
DR TAIR; locus:2193972; AT1G72440.
DR eggNOG; KOG2038; Eukaryota.
DR HOGENOM; CLU_003417_1_1_1; -.
DR InParanoid; F4IDC2; -.
DR OMA; GNHIQYS; -.
DR OrthoDB; 551907at2759; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4IDC2; baseline and differential.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0051302; P:regulation of cell division; IMP:UniProtKB.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR005612; CCAAT-binding_factor.
DR InterPro; IPR040155; CEBPZ/Mak21-like.
DR PANTHER; PTHR12048; PTHR12048; 1.
DR Pfam; PF03914; CBF; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1043
FT /note="Protein SLOW WALKER 2"
FT /id="PRO_0000454494"
FT REGION 32..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 632..737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 861..1043
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 44..51
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 441..448
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 1022..1029
FT /note="Nuclear localization signal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 60..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 642..682
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..698
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 876..981
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 999..1026
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1027..1043
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 633
FT /note="I -> L (in Ref. 1; ABX24524)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1043 AA; 117024 MW; 9CBE6BBB062BE7E9 CRC64;
MSKIKPLSKS SQDLSLLTSD IASFASSIGL ASALPSSGFN DTDFRKPAKS KTQKRKKPKK
DQQHKDEDEE GEPKSNIGNE KGKDFGARKQ NKDAPVKQTL QPKPKPGFLS IDDESTGYKK
KRFDEFKSLP KLPLVKASLL SSEWYNDAAE FEEKVFGGRK VAVANKEDFK GVVEKKRELG
ERLMWQYAED FATSKGKGGD MKMVISAQKS GTVADKITAF EIMVGENPIA NMRSLDALLG
MVTSKVGKRF AFKGLKALSE ILIRLLPDRK LKSLLQRPLN IIPENKDGYS LLLFWYWEDC
LKQRYERFVT ALDESSKDML PELKDKALKT IYFMLTSKSE QERKLLVSLV NKLGDPQNKS
ASNADYHLTN LLADHPNMKA VVIDEVDSFL FRPHLGLRAK YHAVNFLSQI RLSHKGEDPK
VAKRLIDVYF ALFKVLTTEA NRKQGADDKG AADKKKSNPK DTKQEVSTDS PIELDSRILS
ALLTGVNRAF PYVSTDEADD IIESQTPVLF KLVHSANFNV GVQSLMLLDK ISSKNKIVSD
RFYRALYSKL LLPSAMNSSK AEMFIGLLLR AMKNDINIKR VAAFSKRVLQ VALQQPPQYA
CGCLFLLSEV LKSRPPLWKM VVQRESVEEE EDIEHFEDVI EGDDVDPNKK AENDENVVEV
DHDGVEKSSR DGDSSSDDEE ALAIRLSDEE DDNASDDSEE LIRNETPQLE EVMEVSNDME
KRSQPPMRPS SLPGGYDPRH REPSYCNADR ASWWELGVLS KHAHPSVATM AGTLLSGTNI
VYNGNPLNDL SLTAFLDKFM EKKPKQNTWH GGSQIEPSKK LDMSNRVIGA EILSLAEGDV
APEDLVFHKF YVNKMTSTKQ SKKKKKKKLP EEEAAEELYD VNDGDGGENY DSDVEFEAGD
ESDNEEIENM LDDVDDNAVE EEGGEYDYDD LDGVAGEDDE ELVADVSDAE MDTDMDMDLI
DDEDDNNVDD DGTGDGGDDD SDGDDGRSKK KKKEKRKRKS PFASLEEYKH LIDQDEKEDS
KTKRKATSEP TKKKKKKKSK ASE
