SWEET_ECOU3
ID SWEET_ECOU3 Reviewed; 89 AA.
AC P0DMV3;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2015, sequence version 1.
DT 25-MAY-2022, entry version 18.
DE RecName: Full=Sugar transporter SemiSWEET {ECO:0000303|PubMed:25598322};
GN ORFNames=G925_04926 {ECO:0000312|EMBL:EQX17757.1};
OS Escherichia coli (strain UMEA 3162-1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=1281200;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UMEA 3162-1 {ECO:0000312|EMBL:EQX17757.1};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Feldgarden M., Frimodt-Moller N., Leihof R.F., Rasmussen L., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Escherichia coli UMEA 3162-1.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION,
RP SUBUNIT, AND MUTAGENESIS OF PHE-19; PRO-21; GLN-22; MET-39; TYR-40; TRP-50;
RP TYR-53; ARG-57 AND ASN-66.
RX PubMed=25598322; DOI=10.1038/ncomms7112;
RA Lee Y., Nishizawa T., Yamashita K., Ishitani R., Nureki O.;
RT "Structural basis for the facilitative diffusion mechanism by SemiSWEET
RT transporter.";
RL Nat. Commun. 6:6112-6112(2015).
CC -!- FUNCTION: The homodimer mediates transmembrane sugar transport down a
CC concentration gradient. Transport is probably effected by rocking-type
CC movements, where a cargo-binding cavity opens first on one and then on
CC the other side of the membrane. {ECO:0000269|PubMed:25598322}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:25598322}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:25598322}.
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DR EMBL; AWBU01000046; EQX17757.1; -; Genomic_DNA.
DR RefSeq; WP_000379710.1; NZ_KE701773.1.
DR PDB; 4X5M; X-ray; 2.00 A; A/B/C=1-89.
DR PDB; 4X5N; X-ray; 3.00 A; A/B/C/D=1-89.
DR PDBsum; 4X5M; -.
DR PDBsum; 4X5N; -.
DR AlphaFoldDB; P0DMV3; -.
DR SMR; P0DMV3; -.
DR TCDB; 2.A.123.2.16; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR EnsemblBacteria; EQX17757; EQX17757; G925_04926.
DR PATRIC; fig|1281200.3.peg.5089; -.
DR HOGENOM; CLU_135915_2_0_6; -.
DR Proteomes; UP000016035; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR006603; PQ-loop_rpt.
DR Pfam; PF04193; PQ-loop; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..89
FT /note="Sugar transporter SemiSWEET"
FT /id="PRO_0000432579"
FT TRANSMEM 4..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000269|PubMed:25598322"
FT TRANSMEM 35..55
FT /note="Helical; Name=2"
FT /evidence="ECO:0000269|PubMed:25598322"
FT TRANSMEM 60..82
FT /note="Helical; Name=3"
FT /evidence="ECO:0000269|PubMed:25598322"
FT DOMAIN 7..59
FT /note="PQ-loop"
FT /evidence="ECO:0000255"
FT MUTAGEN 19
FT /note="F->A: Decreases sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 21
FT /note="P->A: Strongly decreases sucrose transporter
FT activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 22
FT /note="Q->A: Slightly decreases sucrose transporter
FT activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 39
FT /note="M->A: No effect on sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 40
FT /note="Y->A: Decreases sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 50
FT /note="W->A: Increases sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 50
FT /note="W->F: No effect on sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 53
FT /note="Y->F: Increases sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 57
FT /note="R->A: Increases sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT MUTAGEN 66
FT /note="N->A: Impairs sucrose transporter activity."
FT /evidence="ECO:0000269|PubMed:25598322"
FT HELIX 3..17
FT /evidence="ECO:0007829|PDB:4X5M"
FT HELIX 20..29
FT /evidence="ECO:0007829|PDB:4X5M"
FT HELIX 37..57
FT /evidence="ECO:0007829|PDB:4X5M"
FT HELIX 60..86
FT /evidence="ECO:0007829|PDB:4X5M"
SQ SEQUENCE 89 AA; 9956 MW; 5A9141CD9225263B CRC64;
MDTILLTGLF AAFFTTFAFA PQSIKTIRTR NTEGISVVMY IMFLTGVISW IAYGIMRSDF
AVLIANIVTL FLAAPVLVIT LINRRKKHV
