SWET1_ARATH
ID SWET1_ARATH Reviewed; 247 AA.
AC Q8L9J7; Q9LPL1;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Bidirectional sugar transporter SWEET1 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET1 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 1 {ECO:0000303|PubMed:21107422};
GN Name=SWEET1 {ECO:0000303|PubMed:21107422};
GN OrderedLocusNames=At1g21460 {ECO:0000312|EMBL:AEE30104.1};
GN ORFNames=F24J8.9 {ECO:0000312|EMBL:AAF87899.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION BY PATHOGENS, DISRUPTION PHENOTYPE, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [6]
RP SUBUNIT, INTERACTION WITH SWEET9; SWEET11; SWEET13; SWEET15; SWEET16 AND
RP SWEET17, MUTAGENESIS OF PRO-23; TYR-57; GLY-58 AND GLY-180, AND SUBCELLULAR
RP LOCATION.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [7]
RP REVIEW.
RC STRAIN=cv. Columbia;
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the plasma membrane. Can transport glucose, and, to a lower extent,
CC mannose, fructose and galactose. {ECO:0000269|PubMed:21107422}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9 mM for glucose {ECO:0000269|PubMed:21107422};
CC Vmax=51.7 pmol/sec/mg enzyme with glucose as substrate (for uptake)
CC {ECO:0000269|PubMed:21107422};
CC pH dependence:
CC Optimum pH is 8.5 for uptake. {ECO:0000269|PubMed:21107422};
CC -!- SUBUNIT: Forms homooligomers and heterooligomers with SWEET9, SWEET11,
CC SWEET13, SWEET15, SWEET16 and SWEET17. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:21107422,
CC ECO:0000269|PubMed:24027245}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21107422}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:24027245}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in flowers.
CC {ECO:0000269|PubMed:21107422}.
CC -!- INDUCTION: Slightly induced by the powdery mildew fungus
CC G.cichoracearum. {ECO:0000269|PubMed:21107422}.
CC -!- DISRUPTION PHENOTYPE: Impaired glucose transport activity.
CC {ECO:0000269|PubMed:21107422}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF87899.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC015447; AAF87899.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30104.1; -; Genomic_DNA.
DR EMBL; BT004185; AAO42204.1; -; mRNA.
DR EMBL; BT005476; AAO63896.1; -; mRNA.
DR EMBL; AY088391; AAM65929.1; -; mRNA.
DR PIR; F86347; F86347.
DR RefSeq; NP_564140.1; NM_101997.3.
DR AlphaFoldDB; Q8L9J7; -.
DR SMR; Q8L9J7; -.
DR BioGRID; 23983; 15.
DR DIP; DIP-61791N; -.
DR IntAct; Q8L9J7; 9.
DR STRING; 3702.AT1G21460.1; -.
DR TCDB; 2.A.123.1.9; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR PaxDb; Q8L9J7; -.
DR PRIDE; Q8L9J7; -.
DR EnsemblPlants; AT1G21460.1; AT1G21460.1; AT1G21460.
DR GeneID; 838744; -.
DR Gramene; AT1G21460.1; AT1G21460.1; AT1G21460.
DR KEGG; ath:AT1G21460; -.
DR Araport; AT1G21460; -.
DR TAIR; locus:2026997; AT1G21460.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_1_0_1; -.
DR InParanoid; Q8L9J7; -.
DR OMA; KLWISIE; -.
DR OrthoDB; 1116261at2759; -.
DR PhylomeDB; Q8L9J7; -.
DR PRO; PR:Q8L9J7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L9J7; baseline and differential.
DR Genevisible; Q8L9J7; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Endoplasmic reticulum; Membrane; Reference proteome; Repeat;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..247
FT /note="Bidirectional sugar transporter SWEET1"
FT /id="PRO_0000404102"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..71
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..127
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..187
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 7..94
FT /note="MtN3/slv 1"
FT DOMAIN 130..213
FT /note="MtN3/slv 2"
FT REGION 221..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 23
FT /note="P->T: Loss of sugar transport activity associated
FT with reduced plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:24027245"
FT MUTAGEN 57
FT /note="Y->A: Loss of sugar transport activity associated
FT with reduced plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:24027245"
FT MUTAGEN 58
FT /note="G->D: Loss of sugar transport activity associated
FT with reduced plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:24027245"
FT MUTAGEN 180
FT /note="G->D: Loss of sugar transport activity associated
FT with reduced plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:24027245"
SQ SEQUENCE 247 AA; 27268 MW; E7D5A0CC8DBA725E CRC64;
MNIAHTIFGV FGNATALFLF LAPSITFKRI IKNKSTEQFS GIPYPMTLLN CLLSAWYGLP
FVSKDNTLVS TINGTGAVIE TVYVLIFLFY APKKEKIKIF GIFSCVLAVF ATVALVSLFA
LQGNGRKLFC GLAATVFSII MYASPLSIMR LVVKTKSVEF MPFFLSLFVF LCGTSWFVYG
LIGRDPFVAI PNGFGCALGT LQLILYFIYC GNKGEKSADA QKDEKSVEMK DDEKKQNVVN
GKQDLQV
