SWET5_ARATH
ID SWET5_ARATH Reviewed; 240 AA.
AC Q9FM10; A0MFR4; Q1PDG2;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Bidirectional sugar transporter SWEET5 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET5 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 5 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein VEGETATIVE CELL EXPRESSED 1 {ECO:0000303|PubMed:16055690};
DE Short=AtVEX1 {ECO:0000303|PubMed:16055690};
GN Name=SWEET5 {ECO:0000303|PubMed:21107422};
GN Synonyms=VEX1 {ECO:0000303|PubMed:16055690}; OrderedLocusNames=At5g62850;
GN ORFNames=MQB2.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 30-155.
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-240.
RC STRAIN=cv. Columbia; TISSUE=Flower bud;
RX PubMed=10907847; DOI=10.1093/dnares/7.3.175;
RA Asamizu E., Nakamura Y., Sato S., Tabata S.;
RT "A large scale analysis of cDNA in Arabidopsis thaliana: generation of
RT 12,028 non-redundant expressed sequence tags from normalized and size-
RT selected cDNA libraries.";
RL DNA Res. 7:175-180(2000).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=16055690; DOI=10.1104/pp.104.054213;
RA Engel M.L., Holmes-Davis R., McCormick S.;
RT "Green sperm. Identification of male gamete promoters in Arabidopsis.";
RL Plant Physiol. 138:2124-2133(2005).
RN [6]
RP INDUCTION BY PATHOGENS, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [7]
RP SUBUNIT, AND INTERACTION WITH SWEET6; SWEET8; SWEET9; SWEET11 AND SWEET12.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [8]
RP REVIEW.
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the plasma membrane (By similarity). May play roles in nurturing the
CC male gametophyte (PubMed:25988582). {ECO:0000250|UniProtKB:Q8L9J7,
CC ECO:0000303|PubMed:25988582}.
CC -!- SUBUNIT: Forms homooligomers and heterooligomers with SWEET6, SWEET8,
CC SWEET9, SWEET11 and SWEET12. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8L9J7};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8L9J7}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the vegetative cell during the later
CC stages of pollen development, mostly in tricellular pollen grains, and,
CC to a lower extent, in bicellular pollen grains.
CC {ECO:0000269|PubMed:16055690}.
CC -!- INDUCTION: Slightly induced by the powdery mildew fungus
CC G.cichoracearum and the pathogenic bacteria P.syringae pv. tomato.
CC {ECO:0000269|PubMed:21107422}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10854.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB009053; BAB10854.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97664.1; -; Genomic_DNA.
DR EMBL; DQ447106; ABE66274.1; -; mRNA.
DR EMBL; DQ653391; ABK28775.1; -; mRNA.
DR EMBL; AV533590; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001330980.1; NM_001345563.1.
DR RefSeq; NP_201091.2; NM_125680.3.
DR AlphaFoldDB; Q9FM10; -.
DR SMR; Q9FM10; -.
DR BioGRID; 21649; 34.
DR IntAct; Q9FM10; 27.
DR STRING; 3702.AT5G62850.1; -.
DR PaxDb; Q9FM10; -.
DR PRIDE; Q9FM10; -.
DR ProteomicsDB; 226554; -.
DR EnsemblPlants; AT5G62850.1; AT5G62850.1; AT5G62850.
DR GeneID; 836406; -.
DR Gramene; AT5G62850.1; AT5G62850.1; AT5G62850.
DR KEGG; ath:AT5G62850; -.
DR Araport; AT5G62850; -.
DR TAIR; locus:2170748; AT5G62850.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_1_0_1; -.
DR InParanoid; Q9FM10; -.
DR OMA; VEMIFMA; -.
DR OrthoDB; 1116261at2759; -.
DR PhylomeDB; Q9FM10; -.
DR PRO; PR:Q9FM10; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FM10; baseline and differential.
DR Genevisible; Q9FM10; AT.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..240
FT /note="Bidirectional sugar transporter SWEET5"
FT /id="PRO_0000404106"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..45
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..72
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..190
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 10..95
FT /note="MtN3/slv 1"
FT DOMAIN 133..217
FT /note="MtN3/slv 2"
FT CONFLICT 132
FT /note="M -> I (in Ref. 3; ABK28775)"
FT /evidence="ECO:0000305"
FT CONFLICT 154..155
FT /note="KL -> VG (in Ref. 3; ABK28775)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="K -> V (in Ref. 3; ABE66274)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 240 AA; 27210 MW; 556FC7D63F7B83DB CRC64;
MTDPHTARTI VGIVGNVISF GLFCAPIPTM VKIWKMKSVS EFKPDPYVAT VLNCMMWTFY
GLPFVQPDSL LVITINGTGL FMELVYVTIF FVFATSPVRR KITIAMVIEV IFMAVVIFCT
MYFLHTTKQR SMLIGILCIV FNVIMYAAPL TVMKLVIKTK SVKYMPFFLS LANFMNGVVW
VIYACLKFDP YILIPNGLGS LSGIIQLIIY ITYYKTTNWN DDDEDKEKRY SNAGIELGQA
