SWET8_ARATH
ID SWET8_ARATH Reviewed; 239 AA.
AC Q8LFH5; Q2V2R4; Q9FL06;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Bidirectional sugar transporter SWEET8 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET8 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein RUPTURED POLLEN GRAIN 1 {ECO:0000303|PubMed:18434608};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 8 {ECO:0000303|PubMed:21107422};
GN Name=SWEET8 {ECO:0000303|PubMed:21107422};
GN Synonyms=RPG1 {ECO:0000303|PubMed:18434608}; OrderedLocusNames=At5g40260;
GN ORFNames=MSN9.17, MSN9_160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=18434608; DOI=10.1104/pp.108.118026;
RA Guan Y.-F., Huang X.-Y., Zhu J., Gao J.-F., Zhang H.-X., Yang Z.-N.;
RT "RUPTURED POLLEN GRAIN1, a member of the MtN3/saliva Gene family, is
RT crucial for exine pattern formation and cell integrity of microspores in
RT Arabidopsis.";
RL Plant Physiol. 147:852-863(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=16299181; DOI=10.1104/pp.105.067314;
RA Yu H.-J., Hogan P., Sundaresan V.;
RT "Analysis of the female gametophyte transcriptome of Arabidopsis by
RT comparative expression profiling.";
RL Plant Physiol. 139:1853-1869(2005).
RN [8]
RP INDUCTION BY PATHOGENS, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [9]
RP SUBUNIT, INTERACTION WITH SWEET4; SWEET5; SWEET6; SWEET7; SWEET9; SWEET10;
RP SWEET11; SWEET13; SWEET15; SWEET16 AND SWEET17, AND SUBCELLULAR LOCATION.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [10]
RP REVIEW.
RC STRAIN=cv. Columbia;
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the plasma membrane. Required, in pollen, for microspore cell integrity
CC and primexine pattern formation (PubMed:18434608, PubMed:25988582).
CC {ECO:0000250|UniProtKB:Q8L9J7, ECO:0000269|PubMed:18434608,
CC ECO:0000303|PubMed:25988582}.
CC -!- SUBUNIT: Forms homooligomers and heterooligomers with SWEET4, SWEET5,
CC SWEET6, SWEET7, SWEET9, SWEET10, SWEET11, SWEET13, SWEET15, SWEET16 and
CC SWEET17. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18434608,
CC ECO:0000269|PubMed:24027245}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:18434608}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LFH5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LFH5-2; Sequence=VSP_034712, VSP_034713;
CC -!- TISSUE SPECIFICITY: Expressed in inflorescences, embryo sacs and
CC pollen, and at a lower level in stems. Barely detected in roots, leaves
CC and seedlings. {ECO:0000269|PubMed:16299181,
CC ECO:0000269|PubMed:18434608}.
CC -!- DEVELOPMENTAL STAGE: Expressed during anther development in tapetal
CC cells and microsporocytes during meiosis. When the tapetum degenerates,
CC detected only in pollen grains. {ECO:0000269|PubMed:18434608}.
CC -!- INDUCTION: Induced by the pathogenic bacteria P.syringae pv. tomato.
CC {ECO:0000269|PubMed:21107422}.
CC -!- DISRUPTION PHENOTYPE: Dramatically reduced fertility due to a
CC postmeiotic rupture of microspores. {ECO:0000269|PubMed:18434608}.
CC -!- MISCELLANEOUS: [Isoform 2]: Not detected in inflorescence
CC (PubMed:18434608). {ECO:0000305|PubMed:18434608}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10907.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010699; BAB10907.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94526.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94527.1; -; Genomic_DNA.
DR EMBL; AK118347; BAC42961.1; -; mRNA.
DR EMBL; BT005477; AAO63897.1; -; mRNA.
DR EMBL; AY084840; AAM61405.1; -; mRNA.
DR RefSeq; NP_001031986.1; NM_001036909.1. [Q8LFH5-2]
DR RefSeq; NP_568579.1; NM_123390.3. [Q8LFH5-1]
DR AlphaFoldDB; Q8LFH5; -.
DR SMR; Q8LFH5; -.
DR BioGRID; 19275; 12.
DR IntAct; Q8LFH5; 2.
DR STRING; 3702.AT5G40260.1; -.
DR TCDB; 2.A.123.1.5; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR PaxDb; Q8LFH5; -.
DR PRIDE; Q8LFH5; -.
DR ProteomicsDB; 226555; -. [Q8LFH5-1]
DR EnsemblPlants; AT5G40260.1; AT5G40260.1; AT5G40260. [Q8LFH5-1]
DR EnsemblPlants; AT5G40260.2; AT5G40260.2; AT5G40260. [Q8LFH5-2]
DR GeneID; 834024; -.
DR Gramene; AT5G40260.1; AT5G40260.1; AT5G40260. [Q8LFH5-1]
DR Gramene; AT5G40260.2; AT5G40260.2; AT5G40260. [Q8LFH5-2]
DR KEGG; ath:AT5G40260; -.
DR Araport; AT5G40260; -.
DR TAIR; locus:2173782; AT5G40260.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_1_0_1; -.
DR InParanoid; Q8LFH5; -.
DR OMA; PLTIMYK; -.
DR PhylomeDB; Q8LFH5; -.
DR PRO; PR:Q8LFH5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LFH5; baseline and differential.
DR Genevisible; Q8LFH5; AT.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0052543; P:callose deposition in cell wall; IMP:TAIR.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR GO; GO:0010208; P:pollen wall assembly; IMP:TAIR.
DR GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome; Repeat;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..239
FT /note="Bidirectional sugar transporter SWEET8"
FT /id="PRO_0000343944"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..69
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..135
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 9..98
FT /note="MtN3/slv 1"
FT DOMAIN 134..185
FT /note="MtN3/slv 2"
FT VAR_SEQ 197..209
FT /note="ASNGIGTFLALSQ -> VIMGLEPFWHFLS (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034712"
FT VAR_SEQ 210..239
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034713"
SQ SEQUENCE 239 AA; 26886 MW; 6EFFBDF8A29DAA96 CRC64;
MVDAKQVRFI IGVIGNVISF GLFAAPAKTF WRIFKKKSVE EFSYVPYVAT VMNCMLWVFY
GLPVVHKDSI LVSTINGVGL VIELFYVGVY LMYCGHKKNH RRNILGFLAL EVILVVAIIL
ITLFALKGDF VKQTFVGVIC DVFNIAMYGA PSLAIIKVVK TKSVEYMPFL LSLVCFVNAG
IWTTYSLIFK IDYYVLASNG IGTFLALSQL IVYFMYYKST PKEKTVKPSE VEISATERV
