SWET9_ARATH
ID SWET9_ARATH Reviewed; 258 AA.
AC Q9ZV02; Q8LDE6;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Bidirectional sugar transporter SWEET9 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET9 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 9 {ECO:0000303|PubMed:21107422};
GN Name=SWEET9 {ECO:0000303|PubMed:21107422}; OrderedLocusNames=At2g39060;
GN ORFNames=T7F6.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [5]
RP INTERACTION WITH SWEET1; SWEET5; SWEET8; SWEET11; SWEET13; SWEET16 AND
RP SWEET17.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=24670640; DOI=10.1038/nature13082;
RA Lin I.W., Sosso D., Chen L.-Q., Gase K., Kim S.-G., Kessler D.,
RA Klinkenberg P.M., Gorder M.K., Hou B.-H., Qu X.-Q., Carter C.J.,
RA Baldwin I.T., Frommer W.B.;
RT "Nectar secretion requires sucrose phosphate synthases and the sugar
RT transporter SWEET9.";
RL Nature 508:546-549(2014).
RN [7]
RP REVIEW.
RC STRAIN=cv. Columbia;
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the plasma membrane (By similarity). Nectary-specific sugar transporter
CC required for nectar production by mediating the secretion of sucrose
CC from the nectary parenchyma to the extracellular space.
CC {ECO:0000250|UniProtKB:Q8L9J7, ECO:0000269|PubMed:24670640,
CC ECO:0000303|PubMed:25988582}.
CC -!- SUBUNIT: Forms heterooligomers with SWEET1, SWEET5, SWEET8, SWEET11,
CC SWEET13, SWEET16 and SWEET17. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24670640};
CC Multi-pass membrane protein {ECO:0000269|PubMed:24670640}. Cytoplasmic
CC vesicle membrane {ECO:0000269|PubMed:24670640}. Golgi apparatus, trans-
CC Golgi network membrane {ECO:0000269|PubMed:24670640}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in nectaries, mostly in the
CC lower half of nectary parenchyma. {ECO:0000269|PubMed:24670640}.
CC -!- DEVELOPMENTAL STAGE: Accumulates progressively in floral nectaries
CC during maturation, reaching highest levels during maximal nectar
CC secretion. {ECO:0000269|PubMed:24670640}.
CC -!- DISRUPTION PHENOTYPE: In sweet9-1, reduced nectar production
CC accompanied by starch accumulation in nectaries.
CC {ECO:0000269|PubMed:24670640}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
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DR EMBL; AC005770; AAC79616.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09631.1; -; Genomic_DNA.
DR EMBL; AY086047; AAM63257.1; -; mRNA.
DR PIR; F84812; F84812.
DR RefSeq; NP_181439.1; NM_129463.4.
DR AlphaFoldDB; Q9ZV02; -.
DR SMR; Q9ZV02; -.
DR BioGRID; 3831; 26.
DR IntAct; Q9ZV02; 19.
DR STRING; 3702.AT2G39060.1; -.
DR TCDB; 2.A.123.1.24; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR PaxDb; Q9ZV02; -.
DR PRIDE; Q9ZV02; -.
DR ProteomicsDB; 226536; -.
DR EnsemblPlants; AT2G39060.1; AT2G39060.1; AT2G39060.
DR GeneID; 818492; -.
DR Gramene; AT2G39060.1; AT2G39060.1; AT2G39060.
DR KEGG; ath:AT2G39060; -.
DR Araport; AT2G39060; -.
DR TAIR; locus:2064976; AT2G39060.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_4_0_1; -.
DR InParanoid; Q9ZV02; -.
DR OMA; TNAYMIV; -.
DR OrthoDB; 1116261at2759; -.
DR PhylomeDB; Q9ZV02; -.
DR PRO; PR:Q9ZV02; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZV02; baseline and differential.
DR Genevisible; Q9ZV02; AT.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0032588; C:trans-Golgi network membrane; IDA:UniProtKB.
DR GO; GO:0012506; C:vesicle membrane; IDA:UniProtKB.
DR GO; GO:0008515; F:sucrose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0071836; P:nectar secretion; IMP:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasmic vesicle; Golgi apparatus; Membrane;
KW Reference proteome; Repeat; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..258
FT /note="Bidirectional sugar transporter SWEET9"
FT /id="PRO_0000404109"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..69
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..258
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 10..96
FT /note="MtN3/slv 1"
FT DOMAIN 132..216
FT /note="MtN3/slv 2"
FT CONFLICT 4
FT /note="K -> Q (in Ref. 3; AAM63257)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 28716 MW; 0C58F140C586ADBD CRC64;
MFLKVHEIAF LFGLLGNIVS FGVFLSPVPT FYGIYKKKSS KGFQSIPYIC ALASATLLLY
YGIMKTHAYL IISINTFGCF IEISYLFLYI LYAPREAKIS TLKLIVICNI GGLGLLILLV
NLLVPKQHRV STVGWVCAAY SLAVFASPLS VMRKVIKTKS VEYMPFLLSL SLTLNAVMWF
FYGLLIKDKF IAMPNILGFL FGVAQMILYM MYQGSTKTDL PTENQLANKT DVNEVPIVAV
ELPDVGSDNV EGSVRPMK
