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SWI6_YEAST
ID   SWI6_YEAST              Reviewed;         803 AA.
AC   P09959; D6VYI6;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 216.
DE   RecName: Full=Regulatory protein SWI6;
DE   AltName: Full=Cell-cycle box factor subunit SWI6;
DE   AltName: Full=MBF subunit P90;
DE   AltName: Full=Trans-acting activator of HO endonuclease gene;
GN   Name=SWI6; OrderedLocusNames=YLR182W; ORFNames=L9470.8;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2821408; DOI=10.1038/329651a0;
RA   Breeden L., Nasmyth K.;
RT   "Similarity between cell-cycle genes of budding yeast and fission yeast and
RT   the Notch gene of Drosophila.";
RL   Nature 329:651-654(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=1608451; DOI=10.1038/357508a0;
RA   Dirick L., Moll T., Auer H., Nasmyth K.;
RT   "A central role for SWI6 in modulating cell cycle Start-specific
RT   transcription in yeast.";
RL   Nature 357:508-513(1992).
RN   [5]
RP   INTERACTION WITH STB1.
RX   PubMed=10409718; DOI=10.1128/mcb.19.8.5267;
RA   Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J.;
RT   "Regulation of transcription at the Saccharomyces cerevisiae start
RT   transition by Stb1, a Swi6-binding protein.";
RL   Mol. Cell. Biol. 19:5267-5278(1999).
RN   [6]
RP   NUCLEOCYTOPLASMIC SHUTTLING.
RX   PubMed=12697814; DOI=10.1128/mcb.23.9.3126-3140.2003;
RA   Queralt E., Igual J.C.;
RT   "Cell cycle activation of the Swi6p transcription factor is linked to
RT   nucleocytoplasmic shuttling.";
RL   Mol. Cell. Biol. 23:3126-3140(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   PHOSPHORYLATION AT SER-160.
RX   PubMed=14993267; DOI=10.1128/mcb.24.6.2277-2285.2004;
RA   Geymonat M., Spanos A., Wells G.P., Smerdon S.J., Sedgwick S.G.;
RT   "Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular
RT   localization of Swi6.";
RL   Mol. Cell. Biol. 24:2277-2285(2004).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND THR-182, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-547, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [11]
RP   INTERACTION WITH MSA1.
RX   PubMed=18160399; DOI=10.1074/jbc.m708248200;
RA   Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III,
RA   Wittenberg C.;
RT   "The SBF- and MBF-associated protein Msa1 is required for proper timing of
RT   G1-specific transcription in Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 283:6040-6049(2008).
RN   [12]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [13]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-176; THR-179 AND
RP   THR-182, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
RN   [14]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [15]
RP   X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 188-514.
RX   PubMed=10048928; DOI=10.1038/5845;
RA   Foord R., Taylor I.A., Sedgwick S.G., Smerdon S.J.;
RT   "X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals
RT   variations of the ankyrin fold and has implications for Swi6 function.";
RL   Nat. Struct. Biol. 6:157-165(1999).
CC   -!- FUNCTION: Part of a complex involved in cell-cycle-dependent
CC       transcription. SWI4 and SWI6 are required for formation of the cell-
CC       cycle box factor-DNA complex. The repeated element in the upstream
CC       region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).
CC   -!- SUBUNIT: Component of the transcription complex MCB-binding factor
CC       (MBF) composed of SWI6 and MBP1. Component of the transcription complex
CC       SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA1
CC       and STB1. {ECO:0000269|PubMed:10409718, ECO:0000269|PubMed:18160399}.
CC   -!- INTERACTION:
CC       P09959; P13365: CLN3; NbExp=2; IntAct=EBI-18641, EBI-4490;
CC       P09959; P39678: MBP1; NbExp=4; IntAct=EBI-18641, EBI-10485;
CC       P09959; P25302: SWI4; NbExp=8; IntAct=EBI-18641, EBI-18626;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
CC   -!- PTM: Phosphorylated by CDC28 and dephosphorylated by CDC14.
CC       {ECO:0000269|PubMed:14993267}.
CC   -!- MISCELLANEOUS: Present with 3340 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X06238; CAA29581.1; -; Genomic_DNA.
DR   EMBL; U17246; AAB67460.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09502.1; -; Genomic_DNA.
DR   PIR; S03161; RGBYW6.
DR   RefSeq; NP_013283.1; NM_001182069.1.
DR   PDB; 1SW6; X-ray; 2.10 A; A/B=188-512.
DR   PDB; 2XFV; X-ray; 1.90 A; A/B=2-126.
DR   PDB; 5XW5; X-ray; 1.85 A; C=155-164.
DR   PDBsum; 1SW6; -.
DR   PDBsum; 2XFV; -.
DR   PDBsum; 5XW5; -.
DR   AlphaFoldDB; P09959; -.
DR   SMR; P09959; -.
DR   BioGRID; 31453; 698.
DR   ComplexPortal; CPX-946; SBF transcription complex.
DR   ComplexPortal; CPX-950; MBP transcription complex.
DR   DIP; DIP-598N; -.
DR   ELM; P09959; -.
DR   IntAct; P09959; 57.
DR   MINT; P09959; -.
DR   STRING; 4932.YLR182W; -.
DR   CarbonylDB; P09959; -.
DR   iPTMnet; P09959; -.
DR   MaxQB; P09959; -.
DR   PaxDb; P09959; -.
DR   PRIDE; P09959; -.
DR   EnsemblFungi; YLR182W_mRNA; YLR182W; YLR182W.
DR   GeneID; 850879; -.
DR   KEGG; sce:YLR182W; -.
DR   SGD; S000004172; SWI6.
DR   VEuPathDB; FungiDB:YLR182W; -.
DR   eggNOG; ENOG502QPWC; Eukaryota.
DR   GeneTree; ENSGT00940000173997; -.
DR   HOGENOM; CLU_017827_0_0_1; -.
DR   InParanoid; P09959; -.
DR   OMA; DILMGWI; -.
DR   BioCyc; YEAST:G3O-32306-MON; -.
DR   EvolutionaryTrace; P09959; -.
DR   PRO; PR:P09959; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; P09959; protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0030907; C:MBF transcription complex; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0033309; C:SBF transcription complex; IDA:SGD.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003713; F:transcription coactivator activity; IDA:SGD.
DR   GO; GO:0000082; P:G1/S transition of mitotic cell cycle; IDA:ComplexPortal.
DR   GO; GO:0010845; P:positive regulation of reciprocal meiotic recombination; IMP:SGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR   GO; GO:0061408; P:positive regulation of transcription from RNA polymerase II promoter in response to heat stress; IMP:SGD.
DR   GO; GO:0071931; P:positive regulation of transcription involved in G1/S transition of mitotic cell cycle; IMP:SGD.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:ComplexPortal.
DR   Gene3D; 1.25.40.20; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR040822; Swi6_N.
DR   Pfam; PF13606; Ank_3; 1.
DR   Pfam; PF18530; Swi6_N; 1.
DR   SMART; SM00248; ANK; 2.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 2.
DR   PROSITE; PS50088; ANK_REPEAT; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; ANK repeat; Cytoplasm; DNA-binding; Nucleus;
KW   Phosphoprotein; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..803
FT                   /note="Regulatory protein SWI6"
FT                   /id="PRO_0000067071"
FT   REPEAT          318..346
FT                   /note="ANK 1"
FT   REPEAT          347..383
FT                   /note="ANK 2"
FT   REPEAT          384..469
FT                   /note="ANK 3"
FT   REPEAT          470..498
FT                   /note="ANK 4"
FT   REPEAT          499..514
FT                   /note="ANK 5"
FT   REGION          108..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          169..212
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          266..288
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          512..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        115..136
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        172..186
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..212
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        512..526
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         149
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         160
FT                   /note="Phosphoserine; by CDC28"
FT                   /evidence="ECO:0000269|PubMed:14993267,
FT                   ECO:0007744|PubMed:17287358"
FT   MOD_RES         176
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         179
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         182
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         547
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17287358"
FT   STRAND          3..11
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   STRAND          16..23
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   TURN            24..26
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   STRAND          29..31
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           32..34
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           35..44
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           47..49
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           58..68
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   STRAND          80..82
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           84..93
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   TURN            97..100
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   HELIX           101..103
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   STRAND          104..106
FT                   /evidence="ECO:0007829|PDB:2XFV"
FT   STRAND          214..216
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   TURN            225..227
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           244..258
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           292..302
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           321..327
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           331..339
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           354..360
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           363..366
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           370..377
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           378..382
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           391..399
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           405..421
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           422..424
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   STRAND          427..429
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           448..452
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           455..461
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   TURN            462..464
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           473..480
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           483..491
FT                   /evidence="ECO:0007829|PDB:1SW6"
FT   HELIX           506..509
FT                   /evidence="ECO:0007829|PDB:1SW6"
SQ   SEQUENCE   803 AA;  90560 MW;  9B317FCACEEC493C CRC64;
     MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET NPDSFPTDEE
     RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF QDAFEFEEPE TDQDEEDPSH
     SKLPENKTKS ENSKDNISSK RINNLQDMSL DSDAHRELGS PLKKLKIDTS VIDAESDSTP
     NTARGKPNDD INKGPSGDNE NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV
     VNDNEQKMKL EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN
     DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM GESCLVKAVK
     SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG MTGCSAAAKY YLDILMGWIV
     KKQNRPIQSG TNEKESKPND KNGERKDSIL ENLDLKWIIA NMLNAQDSNG DTCLNIAARL
     GNISIVDALL DYGADPFIAN KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG
     KAKKIRSQLL KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA
     NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE SSGIDWDSSE
     YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI MEQIRNQLPA EKIQSMLPPT
     VLLKARINAY KRNDKHLTNV LDTISTKQSE LENKFRRVLS LCLKIDENKV DNMLDGLLQA
     ISSEDPQDID TDEMQDFLKK HAS
 
 
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