SWIC2_CAEEL
ID SWIC2_CAEEL Reviewed; 449 AA.
AC O02101;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=SWI/SNF chromatin-remodeling accessory subunit 2 {ECO:0000305};
GN Name=swsn-2.2 {ECO:0000312|WormBase:C18E3.2};
GN ORFNames=C18E3.2 {ECO:0000312|WormBase:C18E3.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=23457234; DOI=10.1534/genetics.112.148742;
RA Weinberg P., Flames N., Sawa H., Garriga G., Hobert O.;
RT "The SWI/SNF chromatin remodeling complex selectively affects multiple
RT aspects of serotonergic neuron differentiation.";
RL Genetics 194:189-198(2013).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24402584; DOI=10.1534/g3.113.009852;
RA Large E.E., Mathies L.D.;
RT "Caenorhabditis elegans SWI/SNF subunits control sequential developmental
RT stages in the somatic gonad.";
RL G3 (Bethesda) 4:471-483(2014).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, IDENTIFICATION IN THE
RP SWI/SNF COMPLEX, AND MUTAGENESIS OF 64-PRO--GLU-422.
RX PubMed=26739451; DOI=10.1534/genetics.115.183533;
RA Ertl I., Porta-de-la-Riva M., Gomez-Orte E., Rubio-Pena K.,
RA Aristizabal-Corrales D., Cornes E., Fontrodona L., Osteikoetxea X.,
RA Ayuso C., Askjaer P., Cabello J., Ceron J.;
RT "Functional Interplay of Two Paralogs Encoding SWI/SNF Chromatin-Remodeling
RT Accessory Subunits During Caenorhabditis elegans Development.";
RL Genetics 202:961-975(2016).
CC -!- FUNCTION: Involved in transcriptional activation and repression of
CC select genes by chromatin remodeling (alteration of DNA-nucleosome
CC topology). Component of SWI/SNF chromatin remodeling complexes that
CC carry out key enzymatic activities, changing chromatin structure by
CC altering DNA-histone contacts within a nucleosome in an ATP-dependent
CC manner (By similarity). Probably regulates vulva development through
CC the let-60/Ras pathway (PubMed:26739451). Involved in nuclear
CC reassembly after mitosis and recruitment of nuclear envelope protein,
CC mel-28, to the nuclear periphery in the early embryo and in the adult
CC germline (PubMed:26739451). Involved in gonadogenesis (PubMed:26739451,
CC PubMed:24402584). {ECO:0000250|UniProtKB:Q96GM5,
CC ECO:0000269|PubMed:24402584, ECO:0000269|PubMed:26739451}.
CC -!- SUBUNIT: Component of the multiprotein chromatin-remodeling complexes
CC SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes
CC (Probable). The canonical complex contains a catalytic subunit swsn-4,
CC core subunits swsn-1 and swsn-5, and accessory subunits swsn-3, swsn-6,
CC phf-10, dpff-1, swsn-9 and either ham-3/swsn-2.1 or swsn-2.2
CC (Probable). {ECO:0000305|PubMed:26739451}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:26739451}. Chromosome
CC {ECO:0000269|PubMed:26739451}. Nucleus envelope
CC {ECO:0000269|PubMed:26739451}. Note=Localizes to mitotic chromosomes in
CC the early embryo. {ECO:0000269|PubMed:26739451}.
CC -!- DEVELOPMENTAL STAGE: Expression begins at the gastrulation stage
CC (PubMed:23457234). Expressed broadly at L1 larval stage except in the
CC intestine (PubMed:23457234). Expression decreases in the subsequent
CC larval stages and is absent in adults (PubMed:23457234).
CC {ECO:0000269|PubMed:23457234}.
CC -!- DISRUPTION PHENOTYPE: Some knockouts exhibit maternal-effect embryonic
CC or larval lethality (PubMed:24402584). Germ lines are smaller
CC (PubMed:26739451). RNAi-mediated knockdown reduces brood size
CC (PubMed:26739451). Simultaneous knockouts of ham-3 do not survive
CC beyond the L2 larval stage (PubMed:24402584). Simultaneous RNAi-
CC mediated knockdown of ham-3 at the L1 larval stage causes sterility,
CC while at the L3 larval stage, causes embryonic lethality for the
CC progeny (PubMed:26739451). The sterile animals resulting from
CC simultaneous RNAi-mediated knockdown of ham-3 at the L1 larval stage
CC develop smaller germ lines (PubMed:26739451). Simultaneous RNAi-
CC mediated knockdown of ham-3 also results in vulva protrusion and
CC ectopic expression of egl-17 in cells derived from the vulval precursor
CC cells P5.p and P7.p (PubMed:26739451). {ECO:0000269|PubMed:24402584,
CC ECO:0000269|PubMed:26739451}.
CC -!- SIMILARITY: Belongs to the SMARCD family. {ECO:0000305}.
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DR EMBL; BX284601; CCD65137.1; -; Genomic_DNA.
DR PIR; T15185; T15185.
DR RefSeq; NP_491329.2; NM_058928.5.
DR AlphaFoldDB; O02101; -.
DR SMR; O02101; -.
DR ComplexPortal; CPX-1030; BAF chromatin remodeling complex.
DR ComplexPortal; CPX-1031; PBAF chromatin remodeling complex.
DR IntAct; O02101; 174.
DR STRING; 6239.C18E3.2.1; -.
DR EPD; O02101; -.
DR PaxDb; O02101; -.
DR PeptideAtlas; O02101; -.
DR EnsemblMetazoa; C18E3.2.1; C18E3.2.1; WBGene00015971.
DR GeneID; 172018; -.
DR KEGG; cel:CELE_C18E3.2; -.
DR UCSC; C18E3.2.2; c. elegans.
DR CTD; 172018; -.
DR WormBase; C18E3.2; CE30249; WBGene00015971; swsn-2.2.
DR eggNOG; KOG2570; Eukaryota.
DR GeneTree; ENSGT00970000196486; -.
DR HOGENOM; CLU_023529_0_2_1; -.
DR InParanoid; O02101; -.
DR OMA; PPLYPKF; -.
DR OrthoDB; 1027566at2759; -.
DR PhylomeDB; O02101; -.
DR Reactome; R-CEL-8939243; RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00015971; Expressed in embryo and 4 other tissues.
DR GO; GO:0000793; C:condensed chromosome; IDA:WormBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0016514; C:SWI/SNF complex; ISS:WormBase.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISS:WormBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IC:ComplexPortal.
DR GO; GO:2000781; P:positive regulation of double-strand break repair; IC:ComplexPortal.
DR GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IC:ComplexPortal.
DR GO; GO:2000819; P:regulation of nucleotide-excision repair; IC:ComplexPortal.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR Gene3D; 1.10.245.10; -; 1.
DR InterPro; IPR019835; SWIB_domain.
DR InterPro; IPR036885; SWIB_MDM2_dom_sf.
DR InterPro; IPR003121; SWIB_MDM2_domain.
DR Pfam; PF02201; SWIB; 1.
DR SMART; SM00151; SWIB; 1.
DR SUPFAM; SSF47592; SSF47592; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Chromosome; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..449
FT /note="SWI/SNF chromatin-remodeling accessory subunit 2"
FT /id="PRO_0000451155"
FT DOMAIN 223..300
FT /note="SWIB/MDM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01273"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 64..422
FT /note="Missing: In ok3161; partially lethal. Gonads are
FT smaller than wild type. The few surviving adults have egg-
FT laying defects and a protruding vulva. F1 progeny die at
FT the embryonic stage and the few surviving larvae do not
FT reach adulthood. Embryos exhibit severe defects in early
FT cell divisions that result in nuclei of different sizes."
FT /evidence="ECO:0000269|PubMed:26739451"
SQ SEQUENCE 449 AA; 52266 MW; D12B7C328F10494F CRC64;
MHSQQRPNPQ MNRHPYGTPG SAPQMRRPGG FAGQPPQMHG PRMVAPPAAP LPKKKKYADK
CIHPKIRELE PDAENYMALL ASEQKLDSTL SRKKLDIQEA LKRPSKVKKR LRIYISHTFI
EEKQPEKDTD EASLPMWELR VEGRLLDEQP PAPAIPGQRP VPKRKFSSFF KSLVIELDKE
MYGPDQHLVE WHRTPQTNET DGFQVKRAGD RPVKCRILLL LDNHPAKFKL HPRLAKVLGI
ATETRPKIIE ALWQYIKTHG LQDPQERDII NCDTFLSQCF GVNRMRFMEV PNKLHQLLQQ
TDPLEFNHII QRPKEGQEQV STCYDIDVEM EDPVKQFMHT FVHSPGLAND IQTLDQKCYD
IIEQINELKT RRDFYARFYT EPAEFIKSWV MSQNSDLKTM NELSGDLEAE RFAESYVRPE
TEEGVQRYMF QKVNQKRHEL EQSLGVRSN
