SWM_DROME
ID SWM_DROME Reviewed; 1062 AA.
AC Q9VIV2; B1NLF3; B1NLF4;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Zinc finger protein swm {ECO:0000305};
DE AltName: Full=Protein second mitotic wave missing {ECO:0000312|EMBL:ABV58368.1};
GN Name=swm {ECO:0000312|FlyBase:FBgn0002044};
GN ORFNames=CG10084 {ECO:0000312|FlyBase:FBgn0002044};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:ABV58368.1, ECO:0000312|EMBL:ABV58369.1, ECO:0000312|EMBL:ABV58370.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ASP-921.
RX PubMed=18245841; DOI=10.1534/genetics.107.081638;
RA Casso D.J., Liu S., Iwaki D.D., Ogden S.K., Kornberg T.B.;
RT "A screen for modifiers of hedgehog signaling in Drosophila melanogaster
RT identifies swm and mts.";
RL Genetics 178:1399-1413(2008).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAM11384.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM11384.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM11384.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21203496; DOI=10.1371/journal.pgen.1001254;
RA Yamamoto-Hino M., Kanie Y., Awano W., Aoki-Kinoshita K.F., Yano H.,
RA Nishihara S., Okano H., Ueda R., Kanie O., Goto S.;
RT "Identification of genes required for neural-specific glycosylation using
RT functional genomics.";
RL PLoS Genet. 6:E1001254-E1001254(2010).
CC -!- FUNCTION: Negatively regulates Hedgehog (hh) protein signal in wing
CC development (PubMed:18245841). Regulates neural-specific glycosylation
CC by binding to FucTA mRNA and facilitating its nuclear export in neural
CC cells (PubMed:21203496). {ECO:0000269|PubMed:18245841,
CC ECO:0000269|PubMed:21203496}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18245841}.
CC -!- DEVELOPMENTAL STAGE: Expressed in precellular embryo along the ventral
CC midline and, in germ-band retraction embryos, in segmental stripes.
CC Expressed in third instar larvae in imaginal disks, salivary gland,
CC optic lobe, fat body, wreath cells and gastric caecae of the gut.
CC {ECO:0000269|PubMed:18245841}.
CC -!- DISRUPTION PHENOTYPE: Recessive lethal (PubMed:18245841). Heteroallelic
CC larvae are severely developmentally delayed and most have wing disks
CC smaller than wild-type (PubMed:18245841). Heteroallelic adult flies
CC show defects in wing hair orientation (PubMed:18245841). Defective
CC alpha-1,3-fucosylation (PubMed:21203496). RNAi-mediated knockdown in
CC the eye results in reduced FucTA protein levels (PubMed:21203496).
CC {ECO:0000269|PubMed:18245841, ECO:0000269|PubMed:21203496}.
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DR EMBL; EF601876; ABV58368.1; -; mRNA.
DR EMBL; EF601877; ABV58369.1; -; Genomic_DNA.
DR EMBL; EF601878; ABV58370.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF53812.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN11045.1; -; Genomic_DNA.
DR EMBL; AY095056; AAM11384.1; -; mRNA.
DR RefSeq; NP_609976.1; NM_136132.5.
DR RefSeq; NP_724201.1; NM_165304.2.
DR AlphaFoldDB; Q9VIV2; -.
DR SMR; Q9VIV2; -.
DR IntAct; Q9VIV2; 5.
DR STRING; 7227.FBpp0303743; -.
DR DNASU; 35235; -.
DR EnsemblMetazoa; FBtr0081244; FBpp0080785; FBgn0002044.
DR EnsemblMetazoa; FBtr0081245; FBpp0080786; FBgn0002044.
DR GeneID; 35235; -.
DR KEGG; dme:Dmel_CG10084; -.
DR UCSC; CG10084-RA; d. melanogaster.
DR CTD; 35235; -.
DR FlyBase; FBgn0002044; swm.
DR VEuPathDB; VectorBase:FBgn0002044; -.
DR eggNOG; KOG2135; Eukaryota.
DR GeneTree; ENSGT00510000046929; -.
DR HOGENOM; CLU_006190_1_0_1; -.
DR InParanoid; Q9VIV2; -.
DR OMA; HADQDQL; -.
DR PhylomeDB; Q9VIV2; -.
DR BioGRID-ORCS; 35235; 0 hits in 3 CRISPR screens.
DR ChiTaRS; swm; fly.
DR GenomeRNAi; 35235; -.
DR PRO; PR:Q9VIV2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0002044; Expressed in cleaving embryo and 31 other tissues.
DR ExpressionAtlas; Q9VIV2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IDA:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:InterPro.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:1900364; P:negative regulation of mRNA polyadenylation; IMP:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0046833; P:positive regulation of RNA export from nucleus; IMP:FlyBase.
DR CDD; cd12258; RRM2_RBM26_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR002483; PWI_dom.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR039511; RBM26-like_RRM2.
DR InterPro; IPR045137; RBM26/27.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR14398; PTHR14398; 2.
DR Pfam; PF01480; PWI; 1.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; Metal-binding; mRNA transport; Nucleus;
KW Reference proteome; RNA-binding; Transport; Zinc; Zinc-finger.
FT CHAIN 1..1062
FT /note="Zinc finger protein swm"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438659"
FT DOMAIN 7..75
FT /note="PWI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00627"
FT DOMAIN 561..635
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT ZN_FING 363..391
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 119..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 666..704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 716..741
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 822..847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 886..920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1004..1062
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..327
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..441
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..704
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 823..841
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1020..1037
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 921
FT /note="D->N: In swmF15; recessive lethal and induces
FT reduced fucosylation levels."
FT /evidence="ECO:0000269|PubMed:18245841,
FT ECO:0000269|PubMed:21203496"
FT CONFLICT 402
FT /note="S -> A (in Ref. 1; ABV58369/ABV58370)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1062 AA; 115717 MW; 8A80CF12C504380D CRC64;
MILENSDKLK DWLSVVLEPL CDADSSALAR YVIALLKKDK SDKDLKRIMI EQLDVFLSEE
TTRFVERLFD AIASEEYLTV PAAPLITASS ASTAELTVDQ ELALVIDGPQ DDIEAVLVAD
SPPPPPKDNV IKPDSNQVKL EQASQDAREA EALAFISQEA GIAMHVPTDA KPAFDHKTKD
SHNQQSASNY QHHHVRSASP PGRSSGVSGS GGGGPGGAGL AAGYADKENQ PRDSRRRRAS
LRSRSRSRSR SNERAFRRSR SRDRRVNERE KTQRQFRNKS PPGSQTDNRH HGRRNFDRRR
IGGNADDRPR FGNNKSRRSH SRSMSPERNA RRNQNSPDRV QTAQANLIPA PVAPPAPVEH
PASHPRQRCR DFDEKGYCVR GETCPWDHGV NPVVFEGINN TSLMMSMREY NPDAPEIWAR
SGGPPPGAGQ GPVPPPTQPG QTTINPFSGN VRPTTLMSGS GPSPMGVPNP ADYARNPGAP
PQAAMMPFPF NPTAVTTPLQ RQLIPIPVVD GAPTGGVAEV GKRRFELEDT VAIADVPTKR
KVPINSRLGP RVNPNMQQHN SSLELRKVPR GLNTIAHLNN HFAKFGKIVN IQVSYEGDPE
AAIVTFSTHA EANVAYRSTE AVLNNRFIKV FWHNDSSGAD VGQMNQMGGG GGGGGRKNAS
QYHLHNVPAV PTPNADGAKI SNANPLTEAG AGNIGTPATE QANTAAPASM RLKLNTTTAG
GSAGGAAGAG APGSGRPLNP AANAASIRKK QEEQQKAVHQ LANGLRKRKQ ELLQSYMKQM
KTALELVERM DQQDPQRAPT LQTIKVLQMT IDKLRKEIKA DQDQLQAQMQ QQQQQQQPPV
KKTKEQQKKE LLDMELELIA QQQEGNDTTA IQKRLEELQR NLGVGSAANN KSTHYAPASG
APGGGAGRKR PNLPEGPTRV DRRPKAIVVT GFAAEEADFV LGHFKNFGEI SKHDVDREIP
QLILSYATRL NAEQAVLRGK MYKDKRLQIS WAPVVTPAPA PMAAPVEKSA APGDMSVSLE
NPKQLIQSVS ESESLLGSDT LPELRLEDEE EDEESEDRSW RR
