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SWNH2_METRA
ID   SWNH2_METRA             Reviewed;         331 AA.
AC   E9F8M4;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Dioxygenase swnH2 {ECO:0000303|PubMed:28381497};
DE            EC=1.14.11.- {ECO:0000305|PubMed:28381497};
DE   AltName: Full=Swainsonine biosynthesis gene cluster protein H2 {ECO:0000303|PubMed:28381497};
GN   Name=swnH2 {ECO:0000303|PubMed:28381497}; ORFNames=MAA_08623;
OS   Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS   anisopliae (strain ARSEF 23)).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=655844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA   Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA   Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA   Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA   Wang C.;
RT   "Genome sequencing and comparative transcriptomics of the model
RT   entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL   PLoS Genet. 7:E1001264-E1001264(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA   Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA   St Leger R.J., Wang C.;
RT   "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT   adaptation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN   [3]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=28381497; DOI=10.1534/g3.117.041384;
RA   Cook D., Donzelli B.G., Creamer R., Baucom D.L., Gardner D.R., Pan J.,
RA   Moore N., Jaromczyk J.W., Schardl C.L.;
RT   "Swainsonine biosynthesis genes in diverse symbiotic and pathogenic
RT   fungi.";
RL   G3 (Bethesda) 7:1791-1797(2017).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=32786262; DOI=10.1021/acschembio.0c00466;
RA   Luo F., Hong S., Chen B., Yin Y., Tang G., Hu F., Zhang H., Wang C.;
RT   "Unveiling of Swainsonine Biosynthesis via a Multibranched Pathway in
RT   Fungi.";
RL   ACS Chem. Biol. 15:2476-2484(2020).
CC   -!- FUNCTION: Dioxygenase; part of the gene cluster that mediates the
CC       biosynthesis of swainsonine (SW), a cytotoxic fungal alkaloid and a
CC       potential cancer therapy drug (PubMed:28381497, PubMed:32786262).
CC       Swainsonine production occurs via a multibranched pathway and is
CC       dispensable for fungal colonization of plants and infection of insect
CC       hosts (PubMed:32786262). The first step of swainsonine biosynthesis is
CC       the production of the precursor pipecolic acid (PA) via conversion of
CC       L-lysine (Lys) to 1-piperideine-6-carboxylate (P6C) by the
CC       aminotransferase swnA, the latter being further reduced to PA by the
CC       reductase swnR (PubMed:32786262). PA can be converted from lysine by
CC       both the SW biosynthetic cluster and the unclustered genes such as
CC       lysine cyclodeaminase (PubMed:32786262). The PKS-NRPS hybrid synthetase
CC       swnK uptakes and condensates PA and malonyl-CoA with and without
CC       skipping of the ketoreductase (KR) domain in order to produce 3
CC       intermediates, 1-oxoindolizidine, (1S)-1-hydroxyindolizin, and (1R)-1-
CC       hydroxyindolizine; with the transisomer (1S)-1-hydroxyindolizin being
CC       predominant (PubMed:32786262). The terminal thioester reductase (TE)
CC       domain of swnK is involved in reduction of the thioester bond to
CC       release the intermediate aldehydes (PubMed:32786262). The
CC       oxidoreductase swnN could contribute to the reduction of 1-
CC       oxoindolizidine to (1S)-1-hydroxyindolizin and (1R)-1-
CC       hydroxyindolizine, contributing to the major route of SW production
CC       (Probable). The dioxygenase swnH2 would be responsible for the
CC       oxidization of (1R)-1-hydroxyindolizine into (1R,2S)-1,2-
CC       dihydroxyindolizine and of (1S)-1-hydroxyindolizin to yield both
CC       (1R,2S)-1,2-dihydroxyindolizine and (1S,2S)-1,2-dihydroxyindolizine
CC       (PubMed:32786262). The dioxygenase swnH1 then performs the conversion
CC       of the 1,2-dihydroxyindolizine epimers to SW (PubMed:32786262).
CC       {ECO:0000269|PubMed:28381497, ECO:0000269|PubMed:32786262,
CC       ECO:0000305|PubMed:32786262}.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000250|UniProtKB:Q4WAW9};
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:32786262}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q4WAW9}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of swainsonine as well
CC       as of the 1,2-dihydroxyindolizine epimers intermediates.
CC       {ECO:0000269|PubMed:32786262}.
CC   -!- SIMILARITY: Belongs to the PhyH family. {ECO:0000305}.
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DR   EMBL; ADNJ02000001; EFY95970.1; -; Genomic_DNA.
DR   RefSeq; XP_007824812.1; XM_007826621.1.
DR   AlphaFoldDB; E9F8M4; -.
DR   SMR; E9F8M4; -.
DR   EnsemblFungi; EFY95970; EFY95970; MAA_08623.
DR   GeneID; 19262909; -.
DR   KEGG; maj:MAA_08623; -.
DR   HOGENOM; CLU_047725_0_0_1; -.
DR   Proteomes; UP000002498; Unassembled WGS sequence.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR008775; Phytyl_CoA_dOase.
DR   Pfam; PF05721; PhyH; 1.
PE   3: Inferred from homology;
KW   Dioxygenase; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN           1..331
FT                   /note="Dioxygenase swnH2"
FT                   /id="PRO_0000441184"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         173
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:O14832"
FT   BINDING         175
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:O14832"
FT   BINDING         250
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:O14832"
SQ   SEQUENCE   331 AA;  37049 MW;  2E78351D29AC0A88 CRC64;
     MINSDAQSAQ KQVEVEKPDE KYSAPRLLPP IPDSYQPAKA ITKIPATSSL EDILAILERD
     GGVILTDFVS LQELDKIDEE LEPYTKSSIA DDDSYNNFIG KKTLVIPGLV GKSDTIANIL
     DTNETIDKLL KVILEERYPA VFEQHTEELV VDPLLSICMG FHVGHGSPRQ ALHRDDMIFS
     SKHRPDMKIN EVDGFSCFLA GTRITRENGG TMVILGSHKW EHDRRGRPDE VSFLEMERGS
     AFIFLSTLAH GAGYNTIPGE VRKITNLVFC RGTLRTEENQ FLCVPRSKVL KMSPKMQTLL
     GFKKPAGSWL GMVENEDPAK DLEAIYEKML K
 
 
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