SWNT_METRA
ID SWNT_METRA Reviewed; 496 AA.
AC E9F8M0;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 2.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Transmembrane transporter swnT {ECO:0000303|PubMed:28381497};
DE AltName: Full=Swainsonine biosynthesis gene cluster protein T {ECO:0000303|PubMed:28381497};
GN Name=swnT {ECO:0000303|PubMed:28381497}; ORFNames=MAA_08619;
OS Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS anisopliae (strain ARSEF 23)).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=655844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA Wang C.;
RT "Genome sequencing and comparative transcriptomics of the model
RT entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL PLoS Genet. 7:E1001264-E1001264(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA St Leger R.J., Wang C.;
RT "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT adaptation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN [3]
RP FUNCTION, AND PATHWAY.
RX PubMed=28381497; DOI=10.1534/g3.117.041384;
RA Cook D., Donzelli B.G., Creamer R., Baucom D.L., Gardner D.R., Pan J.,
RA Moore N., Jaromczyk J.W., Schardl C.L.;
RT "Swainsonine biosynthesis genes in diverse symbiotic and pathogenic
RT fungi.";
RL G3 (Bethesda) 7:1791-1797(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32786262; DOI=10.1021/acschembio.0c00466;
RA Luo F., Hong S., Chen B., Yin Y., Tang G., Hu F., Zhang H., Wang C.;
RT "Unveiling of Swainsonine Biosynthesis via a Multibranched Pathway in
RT Fungi.";
RL ACS Chem. Biol. 15:2476-2484(2020).
CC -!- FUNCTION: Transmembrane transporter; part of the gene cluster that
CC mediates the biosynthesis of swainsonine, a cytotoxic fungal alkaloid
CC and a potential cancer therapy drug (PubMed:28381497). Does not mediate
CC the secretion of SW and the exact role of swnT in SW biosynthesis
CC remains to be determined (PubMed:32786262).
CC {ECO:0000269|PubMed:28381497, ECO:0000269|PubMed:32786262}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Leads to lower intracellular accumulation of SW
CC and higher extracellular yield of SW. {ECO:0000269|PubMed:32786262}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.
CC {ECO:0000305}.
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DR EMBL; ADNJ02000001; EFY95966.2; -; Genomic_DNA.
DR RefSeq; XP_007824808.2; XM_007826617.2.
DR AlphaFoldDB; E9F8M0; -.
DR SMR; E9F8M0; -.
DR EnsemblFungi; EFY95966; EFY95966; MAA_08619.
DR GeneID; 19262905; -.
DR KEGG; maj:MAA_08619; -.
DR HOGENOM; CLU_004495_2_4_1; -.
DR Proteomes; UP000002498; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..496
FT /note="Transmembrane transporter swnT"
FT /id="PRO_0000441190"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 467..487
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 225
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 496 AA; 53807 MW; 50C86408F86A0F5E CRC64;
MAKLGTMPPQ HAAKPVDEHA ARLIPEDSVP IQTQKPFTTL SAIGIGYGVT NTAVGIPLIL
STAMPMGGSP QVFWGFLAMA AVGLATATTL AELVSAMPHP GGQYIWVNAL APKRYRRGLS
YTTAMISWVA AVATGSSGNL SVPLNAFSIV TLLQPDFVYR RWMGFAAFQA INVVTCFGAC
FEHALPKLSK AFLLFNVVSV GVIIVALFAM ADARTSAKDF FTTVNTSGWP DGVAFIIGLN
GANWCFSCLD VATHLAEEIP SPGTNIPKAL LWTIFIASTS GLLVVLAVLV NLGPVDVSDY
SGIGIFYRIT GSKAAAIGLW IPVLILVLAS VWSIQTWQSR LAWTISRESG FPLHRHFSKI
FPAPFYTPIW SLVGSAVGTA LFGCLYLASE LAFNSLIATG ILLQYISYSI PTVLVLWQGR
RNFRHGQFWY PRLGLVANFI MLAWTVVAFV FYCFPANAQV RPSQMNYVSG VLVVIATFIA
ALWILYARKN YRVMEI
