ID   SWP1_ENCCU              Reviewed;         450 AA.
AC   Q9XZV1;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Spore wall protein 1;
DE   Flags: Precursor;
GN   Name=SWP1; OrderedLocusNames=ECU10_1660;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Bohne W., Ferguson D.J.P., Kohler K., Gross U.;
RT   "Molecular characterisation of a developmentally expressed spore wall
RT   protein from the human microsporidian Encephalitozoon cuniculi.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [3]
RP   IDENTIFICATION AS AN ANTIGEN, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=10722629; DOI=10.1128/iai.68.4.2268-2275.2000;
RA   Bohne W., Ferguson D.J.P., Kohler K., Gross U.;
RT   "Developmental expression of a tandemly repeated, glycine- and serine-rich
RT   spore wall protein in the microsporidian pathogen Encephalitozoon
RT   cuniculi.";
RL   Infect. Immun. 68:2268-2275(2000).
RN   [4]
RP   IDENTIFICATION AS A MAJOR ANTIGEN.
RX   PubMed=15181572; DOI=10.1086/421117;
RA   van Gool T., Biderre C., Delbac F., Wentink-Bonnema E., Peek R.,
RA   Vivares C.P.;
RT   "Serodiagnostic studies in an immunocompetent individual infected with
RT   Encephalitozoon cuniculi.";
RL   J. Infect. Dis. 189:2243-2249(2004).
RN   [5]
RP   SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX   PubMed=16469199; DOI=10.1017/s0031182005009777;
RA   Taupin V., Metenier G., Delbac F., Vivares C.P., Prensier G.;
RT   "Expression of two cell wall proteins during the intracellular development
RT   of Encephalitozoon cuniculi: an immunocytochemical and in situ
RT   hybridization study with ultrathin frozen sections.";
RL   Parasitology 132:815-825(2006).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: Spore wall component.
CC   -!- SUBCELLULAR LOCATION: Spore, perispore {ECO:0000269|PubMed:10722629,
CC       ECO:0000269|PubMed:16469199}.
CC   -!- DEVELOPMENTAL STAGE: Preferentially synthesized during differentiation
CC       from meronts to sporonts in early sporogony.
CC       {ECO:0000269|PubMed:10722629, ECO:0000269|PubMed:16469199,
CC       ECO:0000269|PubMed:16691553}.
CC   -!- MISCELLANEOUS: SWP1 is a major antigen recognized during host infection
CC       and might be a target for microsporidian infection diagnosis in the
CC       case of immunocompetent persons.
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DR   EMBL; AJ133745; CAB39735.1; -; mRNA.
DR   EMBL; AL590449; CAD25887.1; -; Genomic_DNA.
DR   RefSeq; NP_586283.1; NM_001042116.1.
DR   AlphaFoldDB; Q9XZV1; -.
DR   GeneID; 859934; -.
DR   KEGG; ecu:ECU10_1660; -.
DR   VEuPathDB; MicrosporidiaDB:ECU10_1660; -.
DR   HOGENOM; CLU_036413_0_0_1; -.
DR   OrthoDB; 1420888at2759; -.
DR   Proteomes; UP000000819; Chromosome X.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Reference proteome; Repeat; Signal; Sporulation.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..450
FT                   /note="Spore wall protein 1"
FT                   /id="PRO_0000022457"
FT   REPEAT          358..374
FT                   /note="1"
FT   REPEAT          375..391
FT                   /note="2"
FT   REPEAT          392..408
FT                   /note="3"
FT   REPEAT          409..425
FT                   /note="4"
FT   REPEAT          426..442
FT                   /note="5"
FT   REGION          58..80
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          357..450
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          358..442
FT                   /note="5 X 17 AA tandem repeats of G-S-G-S-G-G-S-S-G-G-S-S-
FT                   G-S-G-S-D"
SQ   SEQUENCE   450 AA;  45873 MW;  5E7071A3E3A6DF60 CRC64;
     MMKLSLLLGL VSFSAVLASE NRRGNCQMCP EGTRYFEKNN LLGRRFKNDV RKLCGDGMSS
     DVHDESSSHS SSSSDCKPRR RRHHRRYEDS CSFGSSDCDD SSTYSSCVSS ECSPPCRPVP
     LNCDYELKTP IINMGERIFE FLKNYEDQYK KAVVLFLTRI LSQIDGFAPS YPSADYEPLI
     EQLETLGVTV PSNMAADLAA LDAAEATSLA GTIRANAQKV IGDLLARVNT MCYLDLMSLV
     TSGLFASQVT SAFSNTQPII TIAGNDLFTK QMAVFQRLPG TLPSAAITAI TNALQANKNN
     FVTFFTTQTT NLQTDVQNAL TALITALTTL TSTTSTEFTQ FANSEIGALT GRIFGSSGSG
     SGGSSGGSSG SGSDGSGSGG SSGGSSGSGS DGSGSGGSSG GSSGSGSDGS GSGGSSGGSS
     GSGSDGSGSG GSSGGSSGSG SGGESGGSSS