ID   SWP3_ENCCU              Reviewed;         221 AA.
AC   Q8SWI4;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Spore wall protein 3;
DE   Flags: Precursor;
GN   Name=SWP3; Synonyms=EnP2; OrderedLocusNames=ECU01_1270;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11157783; DOI=10.1101/gr.164301;
RA   Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M.,
RA   Weissenbach J., Saurin W., Vivares C.P.;
RT   "Sequence and analysis of chromosome I of the amitochondriate intracellular
RT   parasite Encephalitozoon cuniculi (Microspora).";
RL   Genome Res. 11:198-207(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=16368977; DOI=10.1128/iai.74.1.239-247.2006;
RA   Xu Y., Takvorian P.M., Cali A., Wang F., Zhang H.S., Orr G.A., Weiss L.M.;
RT   "Identification of a new spore wall protein from Encephalitozoon
RT   cuniculi.";
RL   Infect. Immun. 74:239-247(2006).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16368098; DOI=10.1016/j.ijpara.2005.10.005;
RA   Peuvel-Fanget I., Polonais V., Brosson D., Texier C., Kuhn L., Peyret P.,
RA   Vivares C.P., Delbac F.;
RT   "EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi
RT   endospore, the chitin-rich inner layer of the microsporidian spore wall.";
RL   Int. J. Parasitol. 36:309-318(2006).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: Spore wall component.
CC   -!- SUBCELLULAR LOCATION: Spore wall {ECO:0000269|PubMed:16368098,
CC       ECO:0000269|PubMed:16368977}. Membrane {ECO:0000269|PubMed:16368098,
CC       ECO:0000269|PubMed:16368977}; Lipid-anchor, GPI-anchor. Cytoplasm
CC       {ECO:0000269|PubMed:16368977}. Note=Is found in the cytoplasm during
CC       the proliferative and early sporogonic stages before localizing to cell
CC       surface and spore wall in sporonts and spores.
CC       {ECO:0000269|PubMed:16368098}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during all sporogonic stages.
CC       {ECO:0000269|PubMed:16368098, ECO:0000269|PubMed:16691553}.
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DR   EMBL; AL391737; CAD25000.1; -; Genomic_DNA.
DR   RefSeq; XP_965965.1; XM_960872.1.
DR   AlphaFoldDB; Q8SWI4; -.
DR   GeneID; 860306; -.
DR   KEGG; ecu:ECU01_1270; -.
DR   VEuPathDB; MicrosporidiaDB:ECU01_1270; -.
DR   HOGENOM; CLU_1245344_0_0_1; -.
DR   OrthoDB; 1596692at2759; -.
DR   Proteomes; UP000000819; Chromosome I.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0031160; C:spore wall; IDA:CACAO.
PE   1: Evidence at protein level;
KW   Cytoplasm; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..192
FT                   /note="Spore wall protein 3"
FT                   /id="PRO_0000377528"
FT   PROPEP          193..221
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000377529"
FT   REGION          150..203
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        150..169
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           192
FT                   /note="GPI-anchor amidated serine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   221 AA;  22506 MW;  2CE1043AD95CA44C CRC64;
     MVRRSLYFLA VMGVVRSSSG LYIPSVVLQE LGIASSQGCL MIAETNNGNF GIVSSGLENP
     VYITESPQGH REVSWQPVRG EDRAVPIYAP SAEEVRESIS SVRGSEPGQY IAPQPTGFVP
     ASTPVFGTIE SASTAGAAVP VEGVFVASTE NPASTGSSST STCPPKGTAG TTDNKGKAGG
     AAADDKSKSS SSSSSKKKKK GAKSLVALGA VATTALFSIV M