SWP82_YEAST
ID SWP82_YEAST Reviewed; 623 AA.
AC P43554; D6VTI1;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=SWI/SNF global transcription activator complex subunit SWP82;
GN Name=SWP82; OrderedLocusNames=YFL049W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, AND ELECTRON MICROSCOPY OF
RP THE SWI/SNF COMPLEX.
RX PubMed=12524530; DOI=10.1038/nsb888;
RA Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L.,
RA Peterson C.L.;
RT "Structural analysis of the yeast SWI/SNF chromatin remodeling complex.";
RL Nat. Struct. Biol. 10:141-145(2003).
RN [6]
RP IDENTIFICATION IN THE SWI/SNF COMPLEX, AND INTERACTION WITH SWI3 AND SNF12.
RX PubMed=16204215; DOI=10.1534/genetics.105.047589;
RA Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.;
RT "The RSC chromatin remodeling complex bears an essential fungal-specific
RT protein module with broad functional roles.";
RL Genetics 172:795-809(2006).
CC -!- FUNCTION: Involved in transcriptional activation. Component of the
CC SWI/SNF complex, an ATP-dependent chromatin remodeling complex, which
CC is required for the positive and negative regulation of gene expression
CC of a large number of genes. It changes chromatin structure by altering
CC DNA-histone contacts within a nucleosome, leading eventually to a
CC change in nucleosome position, thus facilitating or repressing binding
CC of gene-specific transcription factors.
CC -!- SUBUNIT: Interacts with SWI3 and SNF12. Component of the SWI/SNF global
CC transcription activator complex. The 1.14 MDa SWI/SNF complex is
CC composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2,
CC SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3,
CC SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
CC {ECO:0000269|PubMed:16204215}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 3180 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RSC7/SWP82 family. SWP82 subfamily.
CC {ECO:0000305}.
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DR EMBL; D50617; BAA09192.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12391.1; -; Genomic_DNA.
DR PIR; S56206; S56206.
DR RefSeq; NP_116605.1; NM_001179918.1.
DR PDB; 7C4J; EM; 2.89 A; E=1-623.
DR PDB; 7EGM; EM; 3.60 A; J=1-623.
DR PDB; 7EGP; EM; 6.90 A; J=1-623.
DR PDBsum; 7C4J; -.
DR PDBsum; 7EGM; -.
DR PDBsum; 7EGP; -.
DR AlphaFoldDB; P43554; -.
DR SMR; P43554; -.
DR BioGRID; 31098; 162.
DR ComplexPortal; CPX-1150; SWI/SNF chromatin remodelling complex.
DR DIP; DIP-4202N; -.
DR IntAct; P43554; 17.
DR MINT; P43554; -.
DR STRING; 4932.YFL049W; -.
DR MaxQB; P43554; -.
DR PaxDb; P43554; -.
DR PRIDE; P43554; -.
DR EnsemblFungi; YFL049W_mRNA; YFL049W; YFL049W.
DR GeneID; 850495; -.
DR KEGG; sce:YFL049W; -.
DR SGD; S000001845; SWP82.
DR VEuPathDB; FungiDB:YFL049W; -.
DR eggNOG; ENOG502QVDZ; Eukaryota.
DR HOGENOM; CLU_493537_0_0_1; -.
DR InParanoid; P43554; -.
DR OMA; YWQYKAG; -.
DR BioCyc; YEAST:G3O-30416-MON; -.
DR Reactome; R-SCE-4551638; SUMOylation of chromatin organization proteins.
DR PRO; PR:P43554; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43554; protein.
DR GO; GO:0000785; C:chromatin; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0016514; C:SWI/SNF complex; IDA:UniProtKB.
DR GO; GO:0006338; P:chromatin remodeling; IDA:ComplexPortal.
DR GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; IC:ComplexPortal.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IC:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR InterPro; IPR013933; CRC_Rsc7/Swp82.
DR Pfam; PF08624; CRC_subunit; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Chromatin regulator; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..623
FT /note="SWI/SNF global transcription activator complex
FT subunit SWP82"
FT /id="PRO_0000076350"
FT HELIX 32..49
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 53..56
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 122..130
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 188..194
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 205..210
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 231..240
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 283..295
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 328..334
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 443..446
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 451..460
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 463..465
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 466..501
FT /evidence="ECO:0007829|PDB:7C4J"
FT HELIX 512..524
FT /evidence="ECO:0007829|PDB:7C4J"
FT STRAND 551..553
FT /evidence="ECO:0007829|PDB:7C4J"
SQ SEQUENCE 623 AA; 70276 MW; A8AC00CAC8F0ED0E CRC64;
MLGEDEGNTV LEKGNNPSVK QGEVGAVFIV PKILIREHER VILKQILQIL DQDELVQPPL
DKFPYKKLEL PKYIDELKTR DATNTSYKMI QLDAYGEKKV GSNGELFGGR HYLFNTFTFT
AHMGVLLVLL QDVIKVLYQS NATHDEDEFI VQHDQILVME TSEEQTKFLA KNGVIPEESK
GSFKYITARS AFVEFGASVI AGGQRIVDDY WESLAKKQNL SSHQRVFKLS TNLISKISLL
RPSFQNNRIS NANEISANTN NTCTISTSKF ESQYPIVTEQ PSAEIREAYI ENFAKGEHIS
AIVPGQSISG TLELSAQFRV PRYHSKNSFQ QALQMKAMDI PIGRHEELLA QYESQAPDGS
ASISLPNHIP SVNPSNKPIK RMLSSILDIN VSSSKNKKSE ENEMIKPMNK GQHKNNTSLN
INGWKFESLP LKSAENSGKQ QYYRGLPLYE KNTLLERLKQ LTPNEIKELE HLHDAVFVNT
GLQNVRKVRT KKWKKYWQYK AGIPIGLKRS QLDEFKNKYL KDVLAQTSVT TNFNEITNTD
ETITTKRVPN PNFLGNCNIK DFKPPYIYSH VNKVPQNVAG DKTAVKLDTE VKNTNANPVV
ATDPVAAKPD NLANFSNEVA MNN
