SWT16_ARATH
ID SWT16_ARATH Reviewed; 230 AA.
AC Q9LUR4;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Bidirectional sugar transporter SWEET16 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET16 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 16 {ECO:0000303|PubMed:21107422};
GN Name=SWEET16 {ECO:0000303|PubMed:21107422}; OrderedLocusNames=At3g16690;
GN ORFNames=MGL6.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [6]
RP SUBUNIT, AND INTERACTION WITH SWEET1; SWEET7; SWEET8; SWEET9 AND SWEET17.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY SUGARS AND OSMOTIC STRESSES,
RP SUBCELLULAR LOCATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=24028846; DOI=10.1104/pp.113.224972;
RA Klemens P.A., Patzke K., Deitmer J., Spinner L., Le Hir R., Bellini C.,
RA Bedu M., Chardon F., Krapp A., Neuhaus H.E.;
RT "Overexpression of the vacuolar sugar carrier AtSWEET16 modifies
RT germination, growth, and stress tolerance in Arabidopsis.";
RL Plant Physiol. 163:1338-1352(2013).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24381066; DOI=10.1104/pp.113.232751;
RA Guo W.-J., Nagy R., Chen H.-Y., Pfrunder S., Yu Y.-C., Santelia D.,
RA Frommer W.B., Martinoia E.;
RT "SWEET17, a facilitative transporter, mediates fructose transport across
RT the tonoplast of Arabidopsis roots and leaves.";
RL Plant Physiol. 164:777-789(2014).
RN [9]
RP REVIEW.
RC STRAIN=cv. Columbia;
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Mediates both low-affinity uptake and efflux of sugar across
CC the vacuolar membrane. Regulates sugars homeostasis in leaves and roots
CC by exporting/importing them through the tonoplast regarding metabolic
CC demand (PubMed:24028846). Acts as a vacuolar hexose transporter, such
CC as glucose (Glc), fructose (Fru), and sucrose (Suc) (PubMed:25988582,
CC PubMed:24381066, PubMed:24028846). {ECO:0000269|PubMed:24028846,
CC ECO:0000269|PubMed:24381066, ECO:0000303|PubMed:25988582}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9.9 mM for glucose {ECO:0000269|PubMed:24028846};
CC KM=15.7 mM for fructose {ECO:0000269|PubMed:24028846};
CC KM=10.8 mM for sucrose {ECO:0000269|PubMed:24028846};
CC Note=Vmax with glucose as substrate is 0.68 nmol/hour/Xenopus laevis
CC oocyte. Vmax with fructose as substrate is 0.27 nmol/hour/Xenopus
CC laevis oocyte. Vmax with sucrose as substrate is 0.14
CC nmol/hour/Xenopus laevis oocyte. {ECO:0000269|PubMed:24028846};
CC -!- SUBUNIT: Forms homooligomers and heterooligomers with SWEET1, SWEET7,
CC SWEET8, SWEET9 and SWEET17. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:24028846,
CC ECO:0000269|PubMed:24381066, ECO:0000303|PubMed:25988582}; Multi-pass
CC membrane protein {ECO:0000250|UniProtKB:Q8L9J7}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in the cortex of mature roots,
CC and, to a lower extent, in aerial organs such as leaves, stems, and
CC flowers (PubMed:24381066). Mainly present in vascular parenchyma cells,
CC especially in the petiole vasculature, flower stalks and at the base of
CC individual, not fully developed flowers (PubMed:24028846).
CC {ECO:0000269|PubMed:24028846, ECO:0000269|PubMed:24381066}.
CC -!- INDUCTION: Induced by nitrogen. Repressed by glucose (Glc), fructose
CC (Fru), sucrose (Suc), cold, osmotic stress, and nitrogen starvation.
CC {ECO:0000269|PubMed:24028846}.
CC -!- DISRUPTION PHENOTYPE: Increased root sensitivity to high levels of
CC fructose. {ECO:0000269|PubMed:24381066}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB022217; BAB02761.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75852.1; -; Genomic_DNA.
DR EMBL; BT010528; AAQ65151.1; -; mRNA.
DR EMBL; AK222159; BAD95254.1; -; mRNA.
DR RefSeq; NP_001319571.1; NM_001338249.1.
DR AlphaFoldDB; Q9LUR4; -.
DR SMR; Q9LUR4; -.
DR BioGRID; 6255; 12.
DR IntAct; Q9LUR4; 6.
DR STRING; 3702.AT3G16690.1; -.
DR TCDB; 2.A.123.1.23; the sweet, pq-loop, saliva, mtn3 (sweet) family.
DR PaxDb; Q9LUR4; -.
DR EnsemblPlants; AT3G16690.1; AT3G16690.1; AT3G16690.
DR GeneID; 820921; -.
DR Gramene; AT3G16690.1; AT3G16690.1; AT3G16690.
DR KEGG; ath:AT3G16690; -.
DR Araport; AT3G16690; -.
DR TAIR; locus:2089363; AT3G16690.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_4_1_1; -.
DR InParanoid; Q9LUR4; -.
DR PhylomeDB; Q9LUR4; -.
DR PRO; PR:Q9LUR4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUR4; baseline and differential.
DR Genevisible; Q9LUR4; AT.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005353; F:fructose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008515; F:sucrose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IEP:UniProtKB.
DR GO; GO:1902334; P:fructose export from vacuole to cytoplasm; IMP:UniProtKB.
DR GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEP:UniProtKB.
DR GO; GO:0009750; P:response to fructose; IEP:UniProtKB.
DR GO; GO:0009749; P:response to glucose; IEP:UniProtKB.
DR GO; GO:0006970; P:response to osmotic stress; IEP:UniProtKB.
DR GO; GO:0009744; P:response to sucrose; IEP:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Repeat; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..230
FT /note="Bidirectional sugar transporter SWEET16"
FT /id="PRO_0000404116"
FT TOPO_DOM 1..3
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..24
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..69
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..128
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..230
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 6..92
FT /note="MtN3/slv 1"
FT DOMAIN 129..212
FT /note="MtN3/slv 2"
SQ SEQUENCE 230 AA; 25744 MW; 05FCC1266200916C CRC64;
MADLSFYVGV IGNVISVLVF LSPVETFWRI VQRRSTEEYE CFPYICTLMS SSLWTYYGIV
TPGEYLVSTV NGFGALAESI YVLIFLFFVP KSRFLKTVVV VLALNVCFPV IAIAGTRTLF
GDANSRSSSM GFICATLNII MYGSPLSAIK TVVTTRSVQF MPFWLSFFLF LNGAIWGVYA
LLLHDMFLLV PNGMGFFLGI MQLLIYAYYR NAEPIVEDEE GLIPNQPLLA
