SWT17_ARATH
ID SWT17_ARATH Reviewed; 241 AA.
AC Q84WN3; F4JKX8; O23441;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Bidirectional sugar transporter SWEET17 {ECO:0000303|PubMed:21107422};
DE Short=AtSWEET17 {ECO:0000303|PubMed:21107422};
DE AltName: Full=Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 17 {ECO:0000303|PubMed:21107422};
GN Name=SWEET17 {ECO:0000303|PubMed:21107422};
GN OrderedLocusNames=At4g15920 {ECO:0000312|EMBL:AEE83666.1};
GN ORFNames=dl4000c {ECO:0000312|EMBL:CAB10371.1},
GN FCAALL.237 {ECO:0000312|EMBL:CAB78634.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INDUCTION BY PATHOGENS, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21107422; DOI=10.1038/nature09606;
RA Chen L.-Q., Hou B.-H., Lalonde S., Takanaga H., Hartung M.L., Qu X.-Q.,
RA Guo W.-J., Kim J.-G., Underwood W., Chaudhuri B., Chermak D., Antony G.,
RA White F.F., Somerville S.C., Mudgett M.B., Frommer W.B.;
RT "Sugar transporters for intercellular exchange and nutrition of
RT pathogens.";
RL Nature 468:527-532(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION BY LIGHT;
RP COLD AND NITROGEN DEFICIENCY, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=23583552; DOI=10.1016/j.cub.2013.03.021;
RA Chardon F., Bedu M., Calenge F., Klemens P.A.W., Spinner L., Clement G.,
RA Chietera G., Leran S., Ferrand M., Lacombe B., Loudet O., Dinant S.,
RA Bellini C., Neuhaus H.E., Daniel-Vedele F., Krapp A.;
RT "Leaf fructose content is controlled by the vacuolar transporter SWEET17 in
RT Arabidopsis.";
RL Curr. Biol. 23:697-702(2013).
RN [7]
RP SUBUNIT, AND INTERACTION WITH SWEET1; SWEET2; SWEET3; SWEET4; SWEET6;
RP SWEET7; SWEET8; SWEET9; SWEET11; SWEET12; SWEET13; SWEET15 AND SWEET16.
RX PubMed=24027245; DOI=10.1073/pnas.1311244110;
RA Xuan Y.H., Hu Y.B., Chen L.-Q., Sosso D., Ducat D.C., Hou B.-H.,
RA Frommer W.B.;
RT "Functional role of oligomerization for bacterial and plant SWEET sugar
RT transporter family.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3685-E3685(2013).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP INDUCTION BY DARKNESS AND FRUCTOSE, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=24381066; DOI=10.1104/pp.113.232751;
RA Guo W.-J., Nagy R., Chen H.-Y., Pfrunder S., Yu Y.-C., Santelia D.,
RA Frommer W.B., Martinoia E.;
RT "SWEET17, a facilitative transporter, mediates fructose transport across
RT the tonoplast of Arabidopsis roots and leaves.";
RL Plant Physiol. 164:777-789(2014).
RN [9]
RP REVIEW.
RC STRAIN=cv. Columbia;
RX PubMed=25988582; DOI=10.1016/j.pbi.2015.04.005;
RA Eom J.-S., Chen L.-Q., Sosso D., Julius B.T., Lin I.W., Qu X.-Q.,
RA Braun D.M., Frommer W.B.;
RT "SWEETs, transporters for intracellular and intercellular sugar
RT translocation.";
RL Curr. Opin. Plant Biol. 25:53-62(2015).
CC -!- FUNCTION: Acts as a vacuolar hexose transporter (PubMed:25988582).
CC Regulates fructose (Fru) homeostasis in leaves and roots by
CC exporting/importing Fru through the tonoplast regarding metabolic
CC demand (PubMed:23583552, PubMed:24381066).
CC {ECO:0000269|PubMed:23583552, ECO:0000269|PubMed:24381066,
CC ECO:0000303|PubMed:25988582}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=25.6 mM for fructose {ECO:0000269|PubMed:24381066};
CC Note=Vmax with fructose as substrate is 479.8 pmol/min/ul of vacuole.
CC {ECO:0000269|PubMed:24381066};
CC -!- SUBUNIT: Forms homooligomers and heterooligomers with SWEET1, SWEET2,
CC SWEET3, SWEET4, SWEET6, SWEET7, SWEET8, SWEET9, SWEET11, SWEET12,
CC SWEET13, SWEET15 and SWEET16. {ECO:0000269|PubMed:24027245}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:23583552,
CC ECO:0000269|PubMed:24381066, ECO:0000303|PubMed:25988582}; Multi-pass
CC membrane protein {ECO:0000250|UniProtKB:Q8L9J7}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves at low levels, mostly in xylem
CC and parenchyma (PubMed:23583552, PubMed:24381066). Highly expressed in
CC the cortex of roots, predominantly in tips and mature regions,
CC especially in tonoplasts. Accumulates also in cotyledons, stems,
CC flowers, and siliques (PubMed:24381066). {ECO:0000269|PubMed:23583552,
CC ECO:0000269|PubMed:24381066}.
CC -!- INDUCTION: Slightly induced by the fungal pathogen B.cinerea.
CC Accumulates in response to nitrogen deficiency and cold stress.
CC Expression follows a diurnal rhythm with a peak in the middle of the
CC day (PubMed:23583552). Induced in roots by fructose (Fru) and darkness,
CC thus triggering accumulation and release of vacuolar Fru, respectively
CC (PubMed:24381066). {ECO:0000269|PubMed:21107422,
CC ECO:0000269|PubMed:23583552, ECO:0000269|PubMed:24381066}.
CC -!- DISRUPTION PHENOTYPE: High fructose levels in leaves, especially in
CC vacuoles, due to impaired vacuolar fructose export (PubMed:23583552).
CC Increased root sensitivity to high levels of fructose
CC (PubMed:24381066). {ECO:0000269|PubMed:23583552,
CC ECO:0000269|PubMed:24381066}.
CC -!- SIMILARITY: Belongs to the SWEET sugar transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB10371.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78634.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z97339; CAB10371.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161542; CAB78634.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83666.1; -; Genomic_DNA.
DR EMBL; BT002983; AAO22792.1; -; mRNA.
DR PIR; A71425; A71425.
DR RefSeq; NP_193327.5; NM_117684.6.
DR AlphaFoldDB; Q84WN3; -.
DR SMR; Q84WN3; -.
DR BioGRID; 12568; 13.
DR STRING; 3702.AT4G15920.1; -.
DR PaxDb; Q84WN3; -.
DR PRIDE; Q84WN3; -.
DR ProteomicsDB; 226803; -.
DR GeneID; 827274; -.
DR KEGG; ath:AT4G15920; -.
DR Araport; AT4G15920; -.
DR eggNOG; KOG1623; Eukaryota.
DR HOGENOM; CLU_048643_1_0_1; -.
DR InParanoid; Q84WN3; -.
DR OrthoDB; 1116261at2759; -.
DR PhylomeDB; Q84WN3; -.
DR PRO; PR:Q84WN3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q84WN3; baseline and differential.
DR Genevisible; Q84WN3; AT.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0005353; F:fructose transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0051119; F:sugar transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IBA:GO_Central.
DR GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IEP:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:1902334; P:fructose export from vacuole to cytoplasm; IMP:UniProtKB.
DR GO; GO:0015755; P:fructose transmembrane transport; IMP:UniProtKB.
DR GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR GO; GO:0009646; P:response to absence of light; IEP:UniProtKB.
DR GO; GO:0009750; P:response to fructose; IEP:UniProtKB.
DR InterPro; IPR004316; SWEET_sugar_transpr.
DR Pfam; PF03083; MtN3_slv; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Repeat; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..241
FT /note="Bidirectional sugar transporter SWEET17"
FT /id="PRO_0000404117"
FT TOPO_DOM 1..3
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 4..24
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 42..62
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..69
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 70..90
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 95..115
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..128
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 129..149
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 162..182
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..185
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 186..206
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 6..92
FT /note="MtN3/slv 1"
FT DOMAIN 129..212
FT /note="MtN3/slv 2"
FT CONFLICT 99..101
FT /note="DVD -> VVV (in Ref. 4; AAO22792)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="D -> E (in Ref. 3; AEE83666)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 241 AA; 26799 MW; BCC8DABD25E3CC31 CRC64;
MAEASFYIGV IGNVISVLVF LSPVETFWKI VKRRSTEEYK SLPYICTLLG SSLWTYYGIV
TPGEYLVSTV NGFGALVETI YVSLFLFYAP RHLKLKTVDV DAMLNVFFPI AAIVATRSAF
EDEKMRSQSI GFISAGLNII MYGSPLSAMK TVVTTKSVKY MPFWLSFFLF LNGAIWAVYA
LLQHDVFLLV PNGVGFVFGT MQLILYGIYR NAKPVGLSNG LSEIAQDEEE GLTSRVEPLL
S
