SWTIE_ARATH
ID SWTIE_ARATH Reviewed; 2222 AA.
AC F4HRS2; B0ZC48; B0ZC49; B0ZC50; F4HRS0; F4HRS1; Q0WPN9; Q9ZW93;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Protein SWEETIE {ECO:0000303|PubMed:18452589};
GN Name=SWEETIE {ECO:0000303|PubMed:18452589};
GN OrderedLocusNames=At1g67140 {ECO:0000312|Araport:AT1G67140};
GN ORFNames=F5A8.5 {ECO:0000312|EMBL:AAD10656.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1641-2222 (ISOFORM 1/3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-154; 549-667 AND 1901-2094 (ISOFORM
RP 1/2/3).
RC STRAIN=cv. Bla-10, cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0,
RC cv. Da(1)-12, cv. Di-G, cv. Gr-3, cv. Landsberg erecta, cv. Li-3, cv. Mt-0,
RC cv. PHW-1, cv. PHW-32, and cv. Sha;
RX PubMed=18273534; DOI=10.1007/s00239-007-9063-3;
RA Moore R.C., Stevens M.H.H.;
RT "Local patterns of nucleotide polymorphism are highly variable in the
RT selfing species Arabidopsis thaliana.";
RL J. Mol. Evol. 66:116-129(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Root;
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=18452589; DOI=10.1111/j.1365-313x.2008.03541.x;
RA Veyres N., Danon A., Aono M., Galliot S., Karibasappa Y.B., Diet A.,
RA Grandmottet F., Tamaoki M., Lesur D., Pilard S., Boitel-Conti M.,
RA Sangwan-Norreel B.S., Sangwan R.S.;
RT "The Arabidopsis sweetie mutant is affected in carbohydrate metabolism and
RT defective in the control of growth, development and senescence.";
RL Plant J. 55:665-686(2008).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19704840; DOI=10.4161/psb.3.9.6470;
RA Veyres N., Aono M., Sangwan-Norreel B.S., Sangwan R.S.;
RT "Has Arabidopsis SWEETIE protein a role in sugar flux and utilization?";
RL Plant Signal. Behav. 3:722-725(2008).
CC -!- FUNCTION: May regulate multiple metabolic, hormonal and stress-related
CC pathways. Required for carbohydrate metabolism and homoeostasis. May
CC also monitor ethylene biosynthesis and senescence.
CC {ECO:0000269|PubMed:18452589, ECO:0000269|PubMed:19704840}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=F4HRS2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HRS2-2; Sequence=VSP_058998, VSP_058999;
CC Name=3;
CC IsoId=F4HRS2-3; Sequence=VSP_058998;
CC -!- DISRUPTION PHENOTYPE: Drastically altered morphogenesis, growth and
CC development, including severe dwarfism, lancet-shaped leaves, early
CC senescence and flower sterility. Strongly modified carbohydrate
CC metabolism leading to increased accumulation of endogenous sugars (e.g.
CC trehalose, trehalose-6-phosphate and starch). Ethylene over-production.
CC Up-regulation of genes involved in sugar metabolism, senescence,
CC ethylene biosynthesis and abiotic stress. In light, hypersensitivity to
CC sucrose and glucose during vegetative growth, with partial phenotypic
CC reversion in the presence of high sorbitol concentrations. Altered
CC sugar-mediated hypocotyl elongation response in the dark.
CC {ECO:0000269|PubMed:18452589, ECO:0000269|PubMed:19704840}.
CC -!- SIMILARITY: Belongs to the HEATR5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10656.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004146; AAD10656.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34600.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34601.2; -; Genomic_DNA.
DR EMBL; CP002684; AEE34602.1; -; Genomic_DNA.
DR EMBL; AK229024; BAF00910.1; -; mRNA.
DR EMBL; EU351115; ABZ04650.1; -; Genomic_DNA.
DR EMBL; EU351115; ABZ04651.1; -; Genomic_DNA.
DR EMBL; EU351115; ABZ04652.1; -; Genomic_DNA.
DR EMBL; EU351116; ABZ04653.1; -; Genomic_DNA.
DR EMBL; EU351116; ABZ04654.1; -; Genomic_DNA.
DR EMBL; EU351116; ABZ04655.1; -; Genomic_DNA.
DR EMBL; EU351117; ABZ04656.1; -; Genomic_DNA.
DR EMBL; EU351117; ABZ04657.1; -; Genomic_DNA.
DR EMBL; EU351118; ABZ04659.1; -; Genomic_DNA.
DR EMBL; EU351118; ABZ04660.1; -; Genomic_DNA.
DR EMBL; EU351118; ABZ04661.1; -; Genomic_DNA.
DR EMBL; EU351119; ABZ04662.1; -; Genomic_DNA.
DR EMBL; EU351119; ABZ04663.1; -; Genomic_DNA.
DR EMBL; EU351120; ABZ04665.1; -; Genomic_DNA.
DR EMBL; EU351120; ABZ04666.1; -; Genomic_DNA.
DR EMBL; EU351121; ABZ04668.1; -; Genomic_DNA.
DR EMBL; EU351121; ABZ04669.1; -; Genomic_DNA.
DR EMBL; EU351122; ABZ04671.1; -; Genomic_DNA.
DR EMBL; EU351122; ABZ04672.1; -; Genomic_DNA.
DR EMBL; EU351123; ABZ04674.1; -; Genomic_DNA.
DR EMBL; EU351123; ABZ04675.1; -; Genomic_DNA.
DR EMBL; EU351124; ABZ04677.1; -; Genomic_DNA.
DR EMBL; EU351124; ABZ04678.1; -; Genomic_DNA.
DR EMBL; EU351125; ABZ04680.1; -; Genomic_DNA.
DR EMBL; EU351125; ABZ04681.1; -; Genomic_DNA.
DR EMBL; EU351126; ABZ04683.1; -; Genomic_DNA.
DR EMBL; EU351126; ABZ04684.1; -; Genomic_DNA.
DR EMBL; EU351126; ABZ04685.1; -; Genomic_DNA.
DR EMBL; EU351127; ABZ04686.1; -; Genomic_DNA.
DR EMBL; EU351127; ABZ04687.1; -; Genomic_DNA.
DR EMBL; EU351128; ABZ04689.1; -; Genomic_DNA.
DR EMBL; EU351128; ABZ04690.1; -; Genomic_DNA.
DR PIR; C96695; C96695.
DR RefSeq; NP_001185337.1; NM_001198408.1. [F4HRS2-2]
DR RefSeq; NP_001319331.1; NM_001334274.1. [F4HRS2-1]
DR RefSeq; NP_176885.7; NM_105384.8. [F4HRS2-3]
DR AlphaFoldDB; F4HRS2; -.
DR STRING; 3702.AT1G67140.3; -.
DR iPTMnet; F4HRS2; -.
DR MetOSite; F4HRS2; -.
DR PaxDb; F4HRS2; -.
DR PRIDE; F4HRS2; -.
DR ProteomicsDB; 226769; -. [F4HRS2-1]
DR EnsemblPlants; AT1G67140.1; AT1G67140.1; AT1G67140. [F4HRS2-3]
DR EnsemblPlants; AT1G67140.2; AT1G67140.2; AT1G67140. [F4HRS2-2]
DR EnsemblPlants; AT1G67140.3; AT1G67140.3; AT1G67140. [F4HRS2-1]
DR GeneID; 843034; -.
DR Gramene; AT1G67140.1; AT1G67140.1; AT1G67140. [F4HRS2-3]
DR Gramene; AT1G67140.2; AT1G67140.2; AT1G67140. [F4HRS2-2]
DR Gramene; AT1G67140.3; AT1G67140.3; AT1G67140. [F4HRS2-1]
DR KEGG; ath:AT1G67140; -.
DR Araport; AT1G67140; -.
DR TAIR; locus:2033666; AT1G67140.
DR eggNOG; KOG1822; Eukaryota.
DR InParanoid; F4HRS2; -.
DR OMA; ADTHNEE; -.
DR OrthoDB; 71064at2759; -.
DR PRO; PR:F4HRS2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HRS2; baseline and differential.
DR GO; GO:0005975; P:carbohydrate metabolic process; IMP:TAIR.
DR GO; GO:0009693; P:ethylene biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0010364; P:regulation of ethylene biosynthetic process; IMP:UniProtKB.
DR GO; GO:1900055; P:regulation of leaf senescence; IMP:UniProtKB.
DR GO; GO:0005982; P:starch metabolic process; IMP:TAIR.
DR GO; GO:0005991; P:trehalose metabolic process; IMP:TAIR.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR044218; SWEETIE.
DR PANTHER; PTHR46975; PTHR46975; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Ethylene biosynthesis; Reference proteome; Repeat;
KW Stress response.
FT CHAIN 1..2222
FT /note="Protein SWEETIE"
FT /id="PRO_0000440858"
FT REPEAT 37..75
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 228..265
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 331..368
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 510..540
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 541..578
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 611..648
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 768..807
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 898..936
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 968..1008
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 1029..1066
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 1238..1269
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 1270..1306
FT /note="HEAT 12"
FT /evidence="ECO:0000255"
FT REPEAT 1312..1354
FT /note="HEAT 13"
FT /evidence="ECO:0000255"
FT REPEAT 1372..1410
FT /note="HEAT 14"
FT /evidence="ECO:0000255"
FT REPEAT 1434..1474
FT /note="HEAT 15"
FT /evidence="ECO:0000255"
FT REPEAT 1550..1586
FT /note="HEAT 16"
FT /evidence="ECO:0000255"
FT REPEAT 1783..1820
FT /note="HEAT 17"
FT /evidence="ECO:0000255"
FT REPEAT 1836..1874
FT /note="HEAT 18"
FT /evidence="ECO:0000255"
FT REPEAT 1880..1917
FT /note="HEAT 19"
FT /evidence="ECO:0000255"
FT REPEAT 1966..2006
FT /note="HEAT 20"
FT /evidence="ECO:0000255"
FT REGION 1133..1165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1992..2203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1141..1155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1992..2008
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2009..2028
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2029..2043
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2044..2127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2133..2170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2187..2203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1412
FT /note="Missing (in isoform 2 and isoform 3)"
FT /id="VSP_058998"
FT VAR_SEQ 1847
FT /note="L -> LV (in isoform 2)"
FT /id="VSP_058999"
SQ SEQUENCE 2222 AA; 244381 MW; 97E0AAB91AA7843B CRC64;
MTKNIASDNV PLSRFGVLVA QLESIVASAS QKNPDPLLCF EILSDLISAI DEEPKESLLV
TQRKCEDALY SLVTLGARRP VRHLASVAMA KIISNGDSIS IYSRASSLQG FLSDGKRSDP
QRVAGAAQCL GELYRHFGKK ITSGLFETTS IVTKLVKFNE DFVRQEAFIL LHNALEGCGG
TAAATAYSEA YRLITRFSTL DKSFVVRIAA ARCLKAFSNI GGPGLGTSEF DTLASYCVKG
IEDSESSVRD AFAEALGSLL ALGMHPEAHV QPRGKGPFPP AKKLEGGLQR HLILPFTKAV
GSRAKNTRFG LALSWVFFLQ AIRIRYLDSD SELQDYSLPI MDMLRGDSSI DAHALACVLY
ILRVGVIDQM MEPSQRSFSV FLGKQLQSSN ASPSMKIVAL RALSYTLKTL GEVPHEFKEF
FDDTVGAALS HFLDLVRVEA ALTLRALAEV DPTCVGGLTS FAVTTLNALR ESLSFEKGDK
LKTDLASLHG QAATLAALVS ISPGLSLGYP ARLPRSVLEV SKKMLTESRR NVTVASSEKE
AGWLLLSSLL NSMPKEEFGD QDFDILILWT DVFAGNPEHL IKQQAELKSM LSVWSAAIDA
LTAFVRRFVS CNDGILLQPV LANLRSALSC VSTMANKRFS DVKTLVDILI IRILIAYQSI
PDPLAYKSEH QQIIQLCTTP YRDPSGFEES SCLKSLLDKR DAWLGPWIPG RDWFEDELRY
FQGGEDGLAP SVWESKVSSF PLPETVKKTL VNQMVLCFGI MFASQDSQGM LSLLSVIQQC
LKAGKKQQWR TASLTNICAG LLAGLKALHA LRPQQLTTEV LSSGQAIFQN ILTEGDICAS
QRRAACEGLG LLARLGNDIF TARMTRVLLG DLSGVTDPNY GGSIALALGC IHHSAGGMAL
SSLVPATVNS VSSLTKTSVL GLKIWALHGL LLTIEAAGLS FVSHVQAALG LALDILLTEE
SGWIDLSQGI GRLINAIVAV LGPELSPGSI LFSRCKSVIA EISSWQEIPT LLESVCFTQQ
LILFAPQAVS VHIHVKNLLM TLASRQPIIR RLSVSTLRHL VEKDPVSVID EQIEDNLFQM
LDEETDSEIG NLIRSTLIRL LYATCPSRPS RWMLICRNMA LAASAGRSAE TSIAENDPAY
TRENLGDDDE DMVSSSSGKS IRANPDKDKT LRYRTRVFAA ECLSLLPEAV GNDAAHFDIL
LARNLASNRQ SSGDWLVLQL QELISLAYQI STIQFENMRP IGVGLLSTIL EKFKLVADPE
LPGHLLLEQY QAQLLSAVRT ALDANSGPVL LEAGLQLATK IMTSGIIRSD QVAVKRIFSL
LSRPLNDFNE LYYPSFAEWV TSKIKIRLLA AHASLKCYIF TFLRKHHGEV PVEFEALLPM
FSKSSDLLGR YWIQVLKGYS YICLCQNLKK SQCSFLDEIL PHTVSRRLQP CLEEAWPVIL
QALVLDAIPV NHSVEEFSDR SLISTHRMVT LEAEDFQFLW GFAVLVLFQG MHPASSMQVI
PFSSAKIKSS GDSSINESSF QGLKLYEIAL PVFQSLSAGR FFSSGFLSID LCQELLQVLS
YSFHMDSSWD ILAVSVVQQI SQNCPKDFLE SEEFAYSTIE LCLGYLFKIL HRHNEISPDD
GIWDNMLSPL FISIKTLVKR FELKHRLNSA PLAFLLSGYK CIRQVPTDAY LPKALEIVKS
TNDLLLELTR ASSQKPYTDG TNFAADSGFH LRAIFGACLH MVGDLTRDCI NGIQLVDSKR
SGLRKLLQLK LVFCLEQLFS LAKLAYEFDC PVDETNTNSI CIVMLKSCQI SIAAVVKDSN
VQVQATVLQV LKSLVQRYNN PEEKSFVILF VGELIGDIVS LMQRALLKPV NTESVVIAGE
CLRFIMLLQT HSITDELQKG FMSLFLEVVL VVFSKTSDGV SQEVLELRNV AVRLVSHLAQ
LPSSAVHFKD VLLSLPVTHR QQLQDIIRAS VSKDSALAKP KSLVPAMDIK LPAPVVATPE
KVTSTANMVK EEALSTMPTS FNQVSTVESG TDEEEEEEED DDDDDWDTFQ SFPASTNLEG
SESKTESVAE EEPDLPGRSS IQDDESNAEE TDDQHLASDH ATDITREDSN DKSKEVVEEE
TVEPCFTTRE DSVDKSKEVE EETVKPCLIE DALTSQNDKT SSGDHPVEIN EQSVESKNLE
SENIGTDIKL ASTEVESPAL DDLEPQQIQK SPEDESSKEH VGADVIVTEE TIAENKSDVD
YI
