ID   SY1_PHYPO               Reviewed;          64 AA.
AC   P84178;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   11-DEC-2019, entry version 27.
DE   RecName: Full=Putative isoleucine--tRNA ligase;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
DE   Flags: Fragments;
OS   Physarum polycephalum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Myxogastria;
OC   Myxogastromycetidae; Physariida; Physaraceae; Physarum.
OX   NCBI_TaxID=5791;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBUNIT.
RX   PubMed=15840947; DOI=10.1247/csf.29.159;
RA   Shirakawa T., Nakamura A., Kohama K., Hirakata M., Ogihara S.;
RT   "Class-specific binding of two aminoacyl-tRNA synthetases to annexin, a
RT   Ca2+- and phospholipid-binding protein.";
RL   Cell Struct. Funct. 29:159-164(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBUNIT: Member of a complex that includes annexin.
CC       {ECO:0000269|PubMed:15840947}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255}.
CC   -!- CAUTION: The order of the peptides shown is unknown.
CC       {ECO:0000269|PubMed:15840947}.
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DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Direct protein sequencing; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           <1..>64
FT                   /note="Putative isoleucine--tRNA ligase"
FT                   /id="PRO_0000098606"
FT   NON_CONS        10..11
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_CONS        25..26
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_CONS        36..37
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_CONS        47..48
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_CONS        56..57
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:15840947"
FT   NON_TER         64
FT                   /evidence="ECO:0000303|PubMed:15840947"
SQ   SEQUENCE   64 AA;  7116 MW;  D4FEA4CFEB8C7ECE CRC64;
     LSGVRVTDLH AGVNPGDPVE VFYAVKYTPL FNYFLKVMPY STVLTTSLIN ILYXDNVALA
     FXXI