SYCE3_MOUSE
ID SYCE3_MOUSE Reviewed; 88 AA.
AC B5KM66;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Synaptonemal complex central element protein 3;
DE AltName: Full=Testis-specific expressed protein 2;
DE Short=TSEG-2;
GN Name=Syce3; Synonyms=Tseg2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=19323366;
RA Wang Z.Y., Tong Q.S., Zeng F.Q., Liu Y., Gu Z.H., Zheng L.D., Cai J.B.,
RA Jiang G.S.;
RT "Cloning and expression of a novel mouse testis gene TSEG-2.";
RL Zhonghua Nan Ke Xue 15:99-105(2009).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=20407872; DOI=10.1007/s11596-010-0212-3;
RA Hu T., Wang Z., Zeng F., Chen X., Gu Z., Zheng L., Tong Q.;
RT "Expression pattern of testis-specific expressed gene 2 in cryptorchidism
RT model and its role in apoptosis of spermatogenic cells.";
RL J. Huazhong Univ. Sci. Technol. Med. Sci. 30:193-197(2010).
RN [3]
RP FUNCTION, INTERACTION WITH SYCE1 AND SYCE2, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21637789; DOI=10.1371/journal.pgen.1002088;
RA Schramm S., Fraune J., Naumann R., Hernandez-Hernandez A., Hoog C.,
RA Cooke H.J., Alsheimer M., Benavente R.;
RT "A novel mouse synaptonemal complex protein is essential for loading of
RT central element proteins, recombination, and fertility.";
RL PLoS Genet. 7:E1002088-E1002088(2011).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH SPO16.
RX PubMed=30949703; DOI=10.1093/nar/gkz226;
RA Liu H., Huang T., Li M., Li M., Zhang C., Jiang J., Yu X., Yin Y.,
RA Zhang F., Lu G., Luo M.C., Zhang L.R., Li J., Liu K., Chen Z.J.;
RT "SCRE serves as a unique synaptonemal complex fastener and is essential for
RT progression of meiosis prophase I in mice.";
RL Nucleic Acids Res. 47:5670-5683(2019).
RN [5]
RP INTERACTION WITH PSMA8.
RX PubMed=31437213; DOI=10.1371/journal.pgen.1008316;
RA Gomez-H L., Felipe-Medina N., Condezo Y.B., Garcia-Valiente R., Ramos I.,
RA Suja J.A., Barbero J.L., Roig I., Sanchez-Martin M., de Rooij D.G.,
RA Llano E., Pendas A.M.;
RT "The PSMA8 subunit of the spermatoproteasome is essential for proper
RT meiotic exit and mouse fertility.";
RL PLoS Genet. 15:E1008316-E1008316(2019).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), AND SUBUNIT.
RX PubMed=25394919; DOI=10.1038/srep07059;
RA Lu J., Gu Y., Feng J., Zhou W., Yang X., Shen Y.;
RT "Structural insight into the central element assembly of the synaptonemal
RT complex.";
RL Sci. Rep. 4:7059-7059(2014).
CC -!- FUNCTION: Major component of the transverse central element of
CC synaptonemal complexes (SCS), formed between homologous chromosomes
CC during meiotic prophase. Required for chromosome loading of the central
CC element-specific SCS proteins, and for initiating synapsis between
CC homologous chromosomes. Chromosome loading appears to require SYCP1.
CC Required for fertility. May play a role in apoptosis of spermatogenic
CC cells and pathogenesis of cryptorchidism. {ECO:0000269|PubMed:20407872,
CC ECO:0000269|PubMed:21637789}.
CC -!- SUBUNIT: Homodimer (PubMed:25394919). Interacts with SYCE1
CC (PubMed:21637789, PubMed:25394919). Interacts with SYCE2
CC (PubMed:21637789). Interacts with proteasome subunit PSMA8; to
CC participate in meiosis progression during spermatogenesis
CC (PubMed:31437213). Interacts with SPO16 (PubMed:30949703).
CC {ECO:0000269|PubMed:21637789, ECO:0000269|PubMed:25394919,
CC ECO:0000269|PubMed:30949703, ECO:0000269|PubMed:31437213}.
CC -!- INTERACTION:
CC B5KM66; Q9D495: Syce1; NbExp=2; IntAct=EBI-6128737, EBI-6128757;
CC B5KM66; Q505B8: Syce2; NbExp=2; IntAct=EBI-6128737, EBI-6131573;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21637789}. Chromosome
CC {ECO:0000269|PubMed:21637789, ECO:0000269|PubMed:30949703}.
CC Note=Colocalizes with SYCE1 in the central elements.
CC {ECO:0000269|PubMed:21637789}.
CC -!- TISSUE SPECIFICITY: Expression is restricted to spermatocytes and is
CC absent in spermatogonia, spermatids and spermatogonia (at protein
CC level). Expressed in adult testis and embryonic ovary. Expressed in the
CC convoluted seminiferous tubules in spermatogonia and spermatocytes.
CC {ECO:0000269|PubMed:20407872, ECO:0000269|PubMed:21637789}.
CC -!- DEVELOPMENTAL STAGE: Expression in pubertal testes is first detected at
CC day 12 coinciding with the onset of prophase I of meiosis (at protein
CC level). Expression in spermatocytes first detected during zygotene when
CC synapsis is initiated and persists on synapsed regions of homologous
CC chromosomes until diplotene. In pachytene oocytes expression is also
CC localized to synapsed chromosomes. {ECO:0000269|PubMed:21637789}.
CC -!- INDUCTION: Up-regulated in cryptorchid testes.
CC {ECO:0000269|PubMed:20407872, ECO:0000269|PubMed:21637789}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice are characterized by infertility in
CC both sexes as well as complete disruption of synapsis initiation
CC resulting in meiotic arrest. Initiation of meiotic recombination
CC appears normal, but its progression is severely impaired resulting in
CC complete absence of Mlh1 foci. {ECO:0000269|PubMed:21637789}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EU079025; ABU52996.1; -; mRNA.
DR CCDS; CCDS49703.1; -.
DR RefSeq; NP_001156352.1; NM_001162880.1.
DR RefSeq; NP_001156354.1; NM_001162882.1.
DR PDB; 4R3Q; X-ray; 1.90 A; A/B=1-88.
DR PDB; 6H86; X-ray; 1.90 A; A/B=1-88.
DR PDBsum; 4R3Q; -.
DR PDBsum; 6H86; -.
DR AlphaFoldDB; B5KM66; -.
DR SMR; B5KM66; -.
DR IntAct; B5KM66; 2.
DR STRING; 10090.ENSMUSP00000131766; -.
DR iPTMnet; B5KM66; -.
DR PhosphoSitePlus; B5KM66; -.
DR EPD; B5KM66; -.
DR PaxDb; B5KM66; -.
DR PRIDE; B5KM66; -.
DR ProteomicsDB; 258684; -.
DR Antibodypedia; 62384; 12 antibodies from 7 providers.
DR Ensembl; ENSMUST00000109314; ENSMUSP00000104937; ENSMUSG00000078938.
DR Ensembl; ENSMUST00000167959; ENSMUSP00000131766; ENSMUSG00000078938.
DR GeneID; 75459; -.
DR KEGG; mmu:75459; -.
DR UCSC; uc007xgo.2; mouse.
DR CTD; 644186; -.
DR MGI; MGI:1922709; Syce3.
DR VEuPathDB; HostDB:ENSMUSG00000078938; -.
DR eggNOG; ENOG502S4TF; Eukaryota.
DR GeneTree; ENSGT00390000009978; -.
DR HOGENOM; CLU_2548975_0_0_1; -.
DR InParanoid; B5KM66; -.
DR OMA; MVVIRTN; -.
DR OrthoDB; 1545114at2759; -.
DR PhylomeDB; B5KM66; -.
DR BioGRID-ORCS; 75459; 0 hits in 73 CRISPR screens.
DR PRO; PR:B5KM66; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; B5KM66; protein.
DR Bgee; ENSMUSG00000078938; Expressed in seminiferous tubule of testis and 22 other tissues.
DR ExpressionAtlas; B5KM66; baseline and differential.
DR Genevisible; B5KM66; MM.
DR GO; GO:0000801; C:central element; IDA:UniProtKB.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IDA:MGI.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0035234; P:ectopic germ cell programmed cell death; IDA:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IDA:MGI.
DR GO; GO:0051094; P:positive regulation of developmental process; IDA:MGI.
DR GO; GO:2000243; P:positive regulation of reproductive process; IDA:MGI.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR GO; GO:0007130; P:synaptonemal complex assembly; IMP:UniProtKB.
DR InterPro; IPR028145; Synaptonemal_3.
DR PANTHER; PTHR36686; PTHR36686; 1.
DR Pfam; PF15191; Synaptonemal_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Chromosome; Coiled coil; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..88
FT /note="Synaptonemal complex central element protein 3"
FT /id="PRO_0000367275"
FT COILED 8..75
FT /evidence="ECO:0000255"
FT HELIX 14..48
FT /evidence="ECO:0007829|PDB:4R3Q"
FT HELIX 55..83
FT /evidence="ECO:0007829|PDB:4R3Q"
SQ SEQUENCE 88 AA; 10467 MW; A681AB78783046DD CRC64;
MADSDPGERS YDNMLKMLSD LNKDLEKLLE EMEKISVQAT WMAYDMVVMR TNPTLAESMR
RLEDAFLNCK EEMEKNWQEL LTETKRKQ
