SYCN_MOUSE
ID SYCN_MOUSE Reviewed; 134 AA.
AC Q8VCK7;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 3.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Syncollin;
DE Flags: Precursor;
GN Name=Sycn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11839820; DOI=10.1128/mcb.22.5.1545-1554.2002;
RA Antonin W., Wagner M., Riedel D., Brose N., Jahn R.;
RT "Loss of the zymogen granule protein syncollin affects pancreatic protein
RT synthesis and transport but not secretion.";
RL Mol. Cell. Biol. 22:1545-1554(2002).
RN [4]
RP FUNCTION.
RX PubMed=15040787; DOI=10.1042/bj20031726;
RA Waesle B., Hays L.B., Rhodes C.J., Edwardson J.M.;
RT "Syncollin inhibits regulated corticotropin secretion from AtT-20 cells
RT through a reduction in the secretory vesicle population.";
RL Biochem. J. 380:897-905(2004).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15462671; DOI=10.1042/bj20041064;
RA Waesle B., Turvey M., Larina O., Thorn P., Skepper J., Morton A.J.,
RA Edwardson J.M.;
RT "Syncollin is required for efficient zymogen granule exocytosis.";
RL Biochem. J. 385:721-727(2005).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions in exocytosis in pancreatic acinar cells regulating
CC the fusion of zymogen granules with each other. May have a pore-forming
CC activity on membranes and regulate exocytosis in other exocrine
CC tissues. {ECO:0000269|PubMed:11839820, ECO:0000269|PubMed:15040787,
CC ECO:0000269|PubMed:15462671}.
CC -!- SUBUNIT: Monomer and homooligomer; most probably hexameric. Interacts
CC with GP2 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Zymogen granule membrane
CC {ECO:0000250|UniProtKB:O35775}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O35775}; Lumenal side
CC {ECO:0000250|UniProtKB:O35775}. Zymogen granule lumen
CC {ECO:0000250|UniProtKB:O35775}. Note=Associated in a cholesterol-
CC dependent manner with lipid rafts of zymogen granule membranes.
CC {ECO:0000250|UniProtKB:O35775}.
CC -!- PTM: Contains intrachain disulfide bonds. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Mice display reduced rates of protein synthesis
CC and intracellular transport of secretory proteins. According to
CC PubMed:15040787 mice lacking Sycn display atrophy of the pancreas
CC associated with an elevated amylase content.
CC {ECO:0000269|PubMed:11839820, ECO:0000269|PubMed:15462671}.
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DR EMBL; AK003083; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC019567; AAH19567.1; -; mRNA.
DR CCDS; CCDS21048.1; -.
DR RefSeq; NP_080992.3; NM_026716.3.
DR AlphaFoldDB; Q8VCK7; -.
DR SMR; Q8VCK7; -.
DR STRING; 10090.ENSMUSP00000112770; -.
DR PhosphoSitePlus; Q8VCK7; -.
DR MaxQB; Q8VCK7; -.
DR PaxDb; Q8VCK7; -.
DR PeptideAtlas; Q8VCK7; -.
DR PRIDE; Q8VCK7; -.
DR ProteomicsDB; 258685; -.
DR Antibodypedia; 30273; 78 antibodies from 16 providers.
DR DNASU; 68416; -.
DR Ensembl; ENSMUST00000119180; ENSMUSP00000112770; ENSMUSG00000084174.
DR GeneID; 68416; -.
DR KEGG; mmu:68416; -.
DR UCSC; uc009fzf.2; mouse.
DR CTD; 342898; -.
DR MGI; MGI:1915666; Sycn.
DR VEuPathDB; HostDB:ENSMUSG00000084174; -.
DR eggNOG; ENOG502S3UP; Eukaryota.
DR GeneTree; ENSGT00390000014835; -.
DR HOGENOM; CLU_1890586_0_0_1; -.
DR InParanoid; Q8VCK7; -.
DR OMA; PYLPSNW; -.
DR OrthoDB; 1433772at2759; -.
DR PhylomeDB; Q8VCK7; -.
DR TreeFam; TF338021; -.
DR BioGRID-ORCS; 68416; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Sycn; mouse.
DR PRO; PR:Q8VCK7; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8VCK7; protein.
DR Bgee; ENSMUSG00000084174; Expressed in left colon and 39 other tissues.
DR Genevisible; Q8VCK7; MM.
DR GO; GO:0030667; C:secretory granule membrane; ISO:MGI.
DR GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR InterPro; IPR028137; Syncollin.
DR PANTHER; PTHR17503; PTHR17503; 1.
DR Pfam; PF15138; Syncollin; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Disulfide bond; Exocytosis; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000250"
FT CHAIN 22..134
FT /note="Syncollin"
FT /id="PRO_0000072357"
FT CONFLICT 107
FT /note="S -> T (in Ref. 2; AAH19567)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 134 AA; 14595 MW; 03ECEDFA370CD67C CRC64;
MSLLCPLLLA LALVAVPGAH GNCPVPADLK KSDGTRTCAR LYEKSDPYYD NCCQGPVLSV
EPGTDLPYLP SGWSNTASSL VVGQRCEITV WSLPGKHGKT RKFTAGSYPR LEEYRKGIFG
DWSDSISALY CKCY
