ID   SYCN_YERPE              Reviewed;         123 AA.
AC   P61380; P16162;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2004, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Chaperone protein SycN;
GN   Name=sycN; OrderedLocusNames=YPCD1.37c, y5041, y0044, YP_pCD46;
OS   Yersinia pestis.
OG   Plasmid pCD1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KIM5 / Biovar Mediaevalis;
RX   PubMed=9746557; DOI=10.1128/iai.66.10.4611-4623.1998;
RA   Perry R.D., Straley S.C., Fetherston J.D., Rose D.J., Gregor J.,
RA   Blattner F.R.;
RT   "DNA sequencing and analysis of the low-Ca2+-response plasmid pCD1 of
RT   Yersinia pestis KIM5.";
RL   Infect. Immun. 66:4611-4623(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KIM5 / Biovar Mediaevalis;
RX   PubMed=9748454; DOI=10.1128/jb.180.19.5192-5202.1998;
RA   Hu P., Elliott J., McCready P., Skowronski E., Garnes J., Kobayashi A.,
RA   Brubaker R.R., Garcia E.;
RT   "Structural organization of virulence-associated plasmids of Yersinia
RT   pestis.";
RL   J. Bacteriol. 180:5192-5202(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA   Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA   Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA   Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA   Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
RN   [5]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=KIM5 / Biovar Mediaevalis, and KIM8;
RX   PubMed=10094626; DOI=10.1046/j.1365-2958.1998.01110.x;
RA   Day J.B., Plano G.V.;
RT   "A complex composed of SycN and YscB functions as a specific chaperone for
RT   YopN in Yersinia pestis.";
RL   Mol. Microbiol. 30:777-788(1998).
RN   [6]
RP   FUNCTION.
RC   STRAIN=KIM5 / Biovar Mediaevalis, and KIM8;
RX   PubMed=12535078; DOI=10.1046/j.1365-2958.2003.03343.x;
RA   Day J.B., Ferracci F., Plano G.V.;
RT   "Translocation of YopE and YopN into eukaryotic cells by Yersinia pestis
RT   yopN, tyeA, sycN, yscB and lcrG deletion mutants measured using a
RT   phosphorylatable peptide tag and phosphospecific antibodies.";
RL   Mol. Microbiol. 47:807-823(2003).
CC   -!- FUNCTION: Functions as a specific chaperone for YopN. It could
CC       facilitate the secretion and the subsequent translocation of YopN.
CC       {ECO:0000269|PubMed:10094626, ECO:0000269|PubMed:12535078}.
CC   -!- SUBUNIT: Interacts with YscB to form a complex which specifically binds
CC       to YopN. {ECO:0000269|PubMed:10094626}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10094626}. Cell
CC       inner membrane {ECO:0000269|PubMed:10094626}; Peripheral membrane
CC       protein {ECO:0000269|PubMed:10094626}. Note=Not exported across the
CC       inner membrane.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF074612; AAC69794.1; -; Genomic_DNA.
DR   EMBL; AF053946; AAC62567.1; -; Genomic_DNA.
DR   EMBL; AL117189; CAB54914.1; -; Genomic_DNA.
DR   EMBL; AE017043; AAS58565.1; -; Genomic_DNA.
DR   PIR; T43588; T43588.
DR   RefSeq; NP_395171.1; NC_003131.1.
DR   RefSeq; NP_857745.1; NC_004836.1.
DR   RefSeq; NP_857940.1; NC_004839.1.
DR   RefSeq; WP_002229794.1; NZ_WHLN01000049.1.
DR   PDB; 1XKP; X-ray; 1.70 A; B=1-123.
DR   PDBsum; 1XKP; -.
DR   AlphaFoldDB; P61380; -.
DR   SMR; P61380; -.
DR   STRING; 214092.5832457; -.
DR   EnsemblBacteria; AAS58565; AAS58565; YP_pCD46.
DR   GeneID; 66841111; -.
DR   KEGG; ype:YPCD1.37c; -.
DR   KEGG; ypm:YP_pCD46; -.
DR   PATRIC; fig|214092.21.peg.48; -.
DR   eggNOG; ENOG5033E8X; Bacteria.
DR   HOGENOM; CLU_164012_0_0_6; -.
DR   OMA; IQIDFEY; -.
DR   EvolutionaryTrace; P61380; -.
DR   Proteomes; UP000000815; Plasmid pCD1.
DR   Proteomes; UP000001019; Plasmid pCD1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR012673; T3SS_SynN.
DR   TIGRFAMs; TIGR02503; type_III_SycN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Chaperone; Cytoplasm;
KW   Membrane; Plasmid; Reference proteome.
FT   CHAIN           1..123
FT                   /note="Chaperone protein SycN"
FT                   /id="PRO_0000072360"
FT   HELIX           5..14
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   STRAND          25..31
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   TURN            32..34
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   STRAND          35..42
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   STRAND          45..53
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   HELIX           56..58
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   HELIX           59..64
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   TURN            67..70
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   STRAND          81..85
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   TURN            86..88
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   STRAND          89..97
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   HELIX           98..100
FT                   /evidence="ECO:0007829|PDB:1XKP"
FT   HELIX           103..121
FT                   /evidence="ECO:0007829|PDB:1XKP"
SQ   SEQUENCE   123 AA;  13609 MW;  3771F865B6E3D9AD CRC64;
     MSWIEPIISH FCQDLGVPTS SPLSPLIQLE MAQSGTLQLE QHGATLTLWL ARSLAWHRCE
     DAMVKALTLT AAQKSGALPL RAGWLGESQL VLFVSLDERS LTLPLLHQAF EQLLRLQQEV
     LAP