SYC_COXBU
ID SYC_COXBU Reviewed; 460 AA.
AC Q83BL7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Cysteine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00041};
DE EC=6.1.1.16 {ECO:0000255|HAMAP-Rule:MF_00041};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00041};
DE Short=CysRS {ECO:0000255|HAMAP-Rule:MF_00041};
GN Name=cysS {ECO:0000255|HAMAP-Rule:MF_00041}; OrderedLocusNames=CBU_1487;
OS Coxiella burnetii (strain RSA 493 / Nine Mile phase I).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC Coxiella.
OX NCBI_TaxID=227377;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RSA 493 / Nine Mile phase I;
RX PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C.,
RA Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T.,
RA Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M.,
RA Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E.,
RA Fraser C.M., Heidelberg J.F.;
RT "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-
CC cysteinyl-tRNA(Cys); Xref=Rhea:RHEA:17773, Rhea:RHEA-COMP:9661,
CC Rhea:RHEA-COMP:9679, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:78442, ChEBI:CHEBI:78517,
CC ChEBI:CHEBI:456215; EC=6.1.1.16; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00041};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00041};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00041};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00041}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00041}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00041}.
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DR EMBL; AE016828; AAO90984.2; -; Genomic_DNA.
DR RefSeq; NP_820470.2; NC_002971.3.
DR RefSeq; WP_010958260.1; NZ_CDBG01000001.1.
DR PDB; 3TQO; X-ray; 2.30 A; A=1-460.
DR PDBsum; 3TQO; -.
DR AlphaFoldDB; Q83BL7; -.
DR SMR; Q83BL7; -.
DR STRING; 227377.CBU_1487; -.
DR DNASU; 1209397; -.
DR EnsemblBacteria; AAO90984; AAO90984; CBU_1487.
DR GeneID; 1209397; -.
DR KEGG; cbu:CBU_1487; -.
DR PATRIC; fig|227377.7.peg.1488; -.
DR eggNOG; COG0215; Bacteria.
DR HOGENOM; CLU_013528_0_1_6; -.
DR OMA; AKYWMHN; -.
DR EvolutionaryTrace; Q83BL7; -.
DR Proteomes; UP000002671; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR015273; Cys-tRNA-synt_Ia_DALR.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR10890; PTHR10890; 1.
DR Pfam; PF09190; DALR_2; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SMART; SM00840; DALR_2; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR TIGRFAMs; TIGR00435; cysS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Metal-binding; Nucleotide-binding; Protein biosynthesis;
KW Reference proteome; Zinc.
FT CHAIN 1..460
FT /note="Cysteine--tRNA ligase"
FT /id="PRO_0000159390"
FT MOTIF 31..41
FT /note="'HIGH' region"
FT MOTIF 267..271
FT /note="'KMSKS' region"
FT BINDING 29
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00041"
FT BINDING 210
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00041"
FT BINDING 235
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00041"
FT BINDING 239
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00041"
FT BINDING 270
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00041"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:3TQO"
FT TURN 8..11
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 12..15
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 39..57
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 73..81
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 86..104
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 119..131
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 134..137
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:3TQO"
FT TURN 147..149
FT /evidence="ECO:0007829|PDB:3TQO"
FT TURN 151..158
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 182..187
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 201..204
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 206..218
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 220..228
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 229..231
FT /evidence="ECO:0007829|PDB:3TQO"
FT TURN 232..234
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 235..247
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 252..258
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:3TQO"
FT TURN 270..273
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 278..284
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 287..296
FT /evidence="ECO:0007829|PDB:3TQO"
FT STRAND 303..305
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 307..324
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 337..346
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 352..371
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 375..389
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 390..392
FT /evidence="ECO:0007829|PDB:3TQO"
FT HELIX 399..402
FT /evidence="ECO:0007829|PDB:3TQO"
SQ SEQUENCE 460 AA; 53039 MW; 637153FC3624C33B CRC64;
MSVKIFNSLT KQKEIFKPIE SGKVKLYVCG MTVYDYMHIG HGRSWIIFDM VVRYLRMRGY
EVTFVRNITD IDDKIIKRAG ENKESPAALA ERFIQILHED EKALRVLSPD QEPRATQYVP
EIIKLIQKLL DNQYAYTGQN GDVFFDVRRF KDYGKLSHRH LDELQAGARV EVSDSKRDPL
DFVLWKKAKP GEPKWDSPWG EGRPGWHIEC SAMSSSILGQ PFDIHGGGLD LKFPHHENEI
AQSEAGEEKP FVKLWMHAGL LEINKEKMSK SLGNIISIRE ALKESDVEVL RYFLLSGHYR
NPLSYSKENL ENGRLALERF YLALRGLPVV NHEKTSSYTD RFYEAMDDDF NTPIAFALLF
EMVREINRFR DNNQIEKAAV LAAELKCLGN IFGLLQYSPE QFLQGAKKEA DVQEIKKLID
QRNEARAKKD WKTADQIRDQ LTDLGVAIED SSDGTSWRQE
