SYDE_DROME
ID SYDE_DROME Reviewed; 1866 AA.
AC Q9V9S7; C3KGQ2; E1JJ30; E1JJ31; Q8SWW5;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Rho GTPase-activating protein 100F;
DE Short=RhoGAP-100F;
DE AltName: Full=Synapse defective protein 1 homolog;
DE Short=Protein syd-1 homolog;
GN Name=RhoGAP100F; ORFNames=CG1976;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 938-1866 (ISOFORMS C/D).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-719, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: GTPase activator for the Rho-type GTPases by converting them
CC to an inactive GDP-bound state. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=D;
CC IsoId=Q9V9S7-1; Sequence=Displayed;
CC Name=C;
CC IsoId=Q9V9S7-2; Sequence=VSP_041784;
CC Name=B;
CC IsoId=Q9V9S7-3; Sequence=VSP_041785, VSP_041786;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM11366.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF57207.2; -; Genomic_DNA.
DR EMBL; AE014297; ACZ95078.1; -; Genomic_DNA.
DR EMBL; AE014297; ACZ95079.1; -; Genomic_DNA.
DR EMBL; BT082117; ACQ45355.1; -; mRNA.
DR EMBL; AY095038; AAM11366.1; ALT_INIT; mRNA.
DR RefSeq; NP_001163785.1; NM_001170314.2. [Q9V9S7-3]
DR RefSeq; NP_001163786.1; NM_001170315.3. [Q9V9S7-1]
DR RefSeq; NP_651902.2; NM_143645.2. [Q9V9S7-2]
DR AlphaFoldDB; Q9V9S7; -.
DR SMR; Q9V9S7; -.
DR BioGRID; 68599; 13.
DR IntAct; Q9V9S7; 3.
DR STRING; 7227.FBpp0291391; -.
DR iPTMnet; Q9V9S7; -.
DR PaxDb; Q9V9S7; -.
DR PRIDE; Q9V9S7; -.
DR DNASU; 43758; -.
DR EnsemblMetazoa; FBtr0302179; FBpp0291389; FBgn0039883. [Q9V9S7-3]
DR EnsemblMetazoa; FBtr0302180; FBpp0291390; FBgn0039883. [Q9V9S7-2]
DR EnsemblMetazoa; FBtr0302181; FBpp0291391; FBgn0039883. [Q9V9S7-1]
DR GeneID; 43758; -.
DR KEGG; dme:Dmel_CG1976; -.
DR UCSC; CG1976-RA; d. melanogaster. [Q9V9S7-1]
DR CTD; 43758; -.
DR FlyBase; FBgn0039883; RhoGAP100F.
DR VEuPathDB; VectorBase:FBgn0039883; -.
DR eggNOG; KOG1452; Eukaryota.
DR GeneTree; ENSGT01030000234635; -.
DR InParanoid; Q9V9S7; -.
DR PhylomeDB; Q9V9S7; -.
DR Reactome; R-DME-9013148; CDC42 GTPase cycle.
DR Reactome; R-DME-9013149; RAC1 GTPase cycle.
DR Reactome; R-DME-9035034; RHOF GTPase cycle.
DR BioGRID-ORCS; 43758; 0 hits in 3 CRISPR screens.
DR ChiTaRS; RhoGAP100F; fly.
DR GenomeRNAi; 43758; -.
DR PRO; PR:Q9V9S7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039883; Expressed in brain and 15 other tissues.
DR ExpressionAtlas; Q9V9S7; baseline and differential.
DR Genevisible; Q9V9S7; DM.
DR GO; GO:0031594; C:neuromuscular junction; IDA:SynGO.
DR GO; GO:0048786; C:presynaptic active zone; IDA:FlyBase.
DR GO; GO:0098831; C:presynaptic active zone cytoplasmic component; IDA:SynGO.
DR GO; GO:0097060; C:synaptic membrane; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; ISS:UniProtKB.
DR GO; GO:0090630; P:activation of GTPase activity; ISS:UniProtKB.
DR GO; GO:0048846; P:axon extension involved in axon guidance; IMP:FlyBase.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0030030; P:cell projection organization; IBA:GO_Central.
DR GO; GO:0048789; P:cytoskeletal matrix organization at active zone; IMP:FlyBase.
DR GO; GO:0048790; P:maintenance of presynaptic active zone structure; IMP:FlyBase.
DR GO; GO:1990709; P:presynaptic active zone organization; IMP:FlyBase.
DR GO; GO:0045467; P:R7 cell development; IMP:FlyBase.
DR GO; GO:0046578; P:regulation of Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0007266; P:Rho protein signal transduction; ISM:FlyBase.
DR GO; GO:0007416; P:synapse assembly; IDA:SynGO.
DR GO; GO:0097479; P:synaptic vesicle localization; IMP:FlyBase.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF49562; SSF49562; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1866
FT /note="Rho GTPase-activating protein 100F"
FT /id="PRO_0000312161"
FT DOMAIN 179..264
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 789..908
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 948..1148
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 22..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 262..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 745..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1273..1328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1356..1380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1393..1479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1514..1607
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1644..1727
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1819..1840
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..324
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 508..530
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 749..766
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1293..1318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1356..1379
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1393..1428
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1440..1479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1539..1607
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1644..1662
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1669..1727
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 719
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..27
FT /note="MQWKKKFTRLKAATGNSRVRRMLCCGR -> MCDSATTGCFLTRSSH (in
FT isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_041784"
FT VAR_SEQ 1185..1191
FT /note="RRGESTG -> NVLPASD (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_041785"
FT VAR_SEQ 1192..1866
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_041786"
SQ SEQUENCE 1866 AA; 198298 MW; A156B00E25BE412A CRC64;
MQWKKKFTRL KAATGNSRVR RMLCCGRRKE NGRSVPDVTA SPGRAPPGPL PANQMPAMGN
QQHHGNQQHH GNQQQHHGNQ HSNHRGQSGS LSNAAGVKDP VMLQGDFRKV SGISSEIFRQ
IEAVENDHDP NTAAALEAVE RRGEMIVRVL EPRCMGSKQA VDAAHKLMNK ADARHTVQLV
EIVKRPGQTL GLYIREGNGA DRTDGVFISR IALESAVYNS GCLRVGDEIL AVNLVDVTHM
SLDDVVIIMS IPRRLVLAIR QRRGNRGTGS PGPPTLSRPE QKPPPVVVIK RDLRDEDLDE
TDRMPRPRSS RDRRTGDGRE MTESRSRLGL GLNNYSPQSE QLDMYYNTRG GGGGAMGEPP
NWGYKPPPPP SSVITEQPTK AHAFAPSHAY YQNAGTLESL AEKVHAFYPG QPGGPPVGPS
RRMSTGTGNV GLAQQHARFP RSGSDQHLPR VEYSDYSNSL GRHSLLRSSL KPGTTGGAPM
QVGVGGTLGR YGRYDQQRAG VSKYGPPSGG AQSLTRRSRP NLDYSSDTEA TIGPRPSYYY
YNRPAIGSMS RGSGGAGGGV GAASTAALLA GAADLNKFNS LPRERPGTRL QGIRSRMGDR
LVDENDGNTS APEFDVRRGR DLRQRITASP SIFTADEYRA WLRRAPSSSA IAEQMRMTRD
MFAQPRAQRF SCSAENIHDA LRNTESIYSS RNHILGTGTL DRNMGLTRPI SALPVRSMSS
QHIGGAGSIR SPSIRRMRQL LELSAGPASP SGSILSTGGH QSPAPTPSAT LPRPHRQIDI
NPAEFAKYKL DKPIVDIGGI SGMLWIHLLA GRGLRTAPEG AAGTATQGQT RDLYCVIECD
RVHKARTVVR SGDLQFDWDE SFELDLVGNK QLDVLVYSWD PQHRHKLCYR GAISLSSILR
QSPLHQLALK VEPRGTIYIR MRHTDPLALY KRRGLPSLRA GYPTLFGADL ETVVNRESKN
APGSAPVPIV LRRCVEEVER RGLDIIGLYR LCGSATKKRL LREAFERNSR AVELTPEHVP
DINVITGVLK DYLRELPEPL FTRCLFQMTV DALAVCLPDD PEGNAKLMLS ILDCLPRANR
ATLVFLLDHL SLVVSNSERN KMSAQALATV MGPPLMLHSA SAQPGADIDH AQPIAVLKYL
LQIWPQPQAQ HQQMAQHMGG AAGAMMGGLV TAGSMSNMAG VASGRRGEST GQRGSKVSAL
PADRQQLLLQ QQAQLMAAGN LLRSSTSVTN ILSQGHPQLS ATANNHLYQS VVGQLAQSHR
ALQQAVQQPY QLGGSVGSAI PDPSPLPLPG TPSPGSSSAS TGSGSGSGKS TDTIKRGASP
VSVKQVKIVD QPSSPYSIVM KKPPLQKDAP VEITTPTTQA DTESTLGCKE SNGTASRRGN
VDFYDTHKTQ AKSVVNEESS YSSKYTGSET KKIIPGNSSY TPSKANASGL SGGEDYKAMR
NKSSATSSSS SSQATVLSAG STATSAPTTS SDDSDDLVSY KSSASTNALL AQSQAMTTSQ
LMSKYLKREP RVQFTPIKSP ESPSPPGSGD GLPKGTYQLV TPISGSSSKP GATTGAISKY
TTGSVESSIN ANSQKLSSPS RLCNSKDSNS RTGTASSTTP ATSMVSTGRR LFDSLASSSS
SETETKTYIG GTTAASGAIT TTIYTNDTKN SGSSSSKSGI GGGSGTGLGA VSGASSETRS
FGSTLFGSSG LGNGNGSSHN HSSASPSPFT TTNGNGNHNT MHLYGTLPKN GTSTGAALFG
GSANSSSYHS SASGSGAGTA SSSGVSSMTG STNSYDFYTS TSSTVSSSRP FANGGNNYHT
LGTYRAQYAA TNPFLDAFDE KPGSNGGNAH GEEKLGADKG HHRAAVMAFQ SSGDSKNGSD
EYDDIK
