ID   BIOM_RHOCB              Reviewed;         234 AA.
AC   D5ARH0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Biotin transport ATP-binding protein BioM;
DE            EC=7.6.2.-;
DE   AltName: Full=ECF transporter A component BioM;
GN   Name=bioM; OrderedLocusNames=RCAP_rcc03251;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
RN   [2]
RP   EXPRESSION IN E.COLI, FUNCTION IN BIOTIN UPTAKE, SUBUNIT, SUBCELLULAR
RP   LOCATION, AND MUTAGENESIS OF LYS-41.
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=17301237; DOI=10.1073/pnas.0609905104;
RA   Hebbeln P., Rodionov D.A., Alfandega A., Eitinger T.;
RT   "Biotin uptake in prokaryotes by solute transporters with an optional ATP-
RT   binding cassette-containing module.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2909-2914(2007).
CC   -!- FUNCTION: Required for biotin uptake under very low (pM) biotin
CC       concentrations but not under higher (nM) concentrations.
CC       {ECO:0000269|PubMed:17301237}.
CC   -!- SUBUNIT: Part of a biotin transporter holocomplex composed of BioM,
CC       BioN and BioY. BioMN complexes can be readily purified, but not BioMY
CC       complexes. Only the BioMNY complex has ATPase activity.
CC       {ECO:0000269|PubMed:17301237}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:17301237}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:17301237}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; CP001312; ADE86975.1; -; Genomic_DNA.
DR   RefSeq; WP_013068948.1; NC_014034.1.
DR   AlphaFoldDB; D5ARH0; -.
DR   SMR; D5ARH0; -.
DR   STRING; 272942.RCAP_rcc03251; -.
DR   TCDB; 3.A.1.25.7; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; ADE86975; ADE86975; RCAP_rcc03251.
DR   GeneID; 31492032; -.
DR   KEGG; rcp:RCAP_rcc03251; -.
DR   eggNOG; COG1122; Bacteria.
DR   HOGENOM; CLU_000604_1_22_5; -.
DR   OMA; MSILAME; -.
DR   OrthoDB; 1713578at2; -.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Translocase; Transport.
FT   CHAIN           1..234
FT                   /note="Biotin transport ATP-binding protein BioM"
FT                   /id="PRO_0000409019"
FT   DOMAIN          1..230
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         35..42
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   MUTAGEN         41
FT                   /note="K->N: Diminished biotin uptake, loss of ATPase
FT                   activity, BioMNY complexes still form."
FT                   /evidence="ECO:0000269|PubMed:17301237"
SQ   SEQUENCE   234 AA;  25114 MW;  84FCE533FCC4541E CRC64;
     MQAIDIGHVT LERDGTGVFS DLTLRLTERR IGIVGRNGAG KSSLIRLITG LVTPQKGRVV
     VNGVDVAADR AGALGTVGLL FQNPDHQIIF PVVRDEIAFG LEQKGLKRAA ALARAEAVLA
     AQGRADWGDR LCHTLSQGQR QLLCLMSILA MEPDWILFDE PFNALDLPTA LSIEARIAGL
     AQNVVLVTHD PSRLTGFDRI LWLEGGRIEA DGPPAEVLPR YIAAMQALAR AGAC