BIOP_ECO57
ID BIOP_ECO57 Reviewed; 299 AA.
AC P0ADP6; P27849; Q8X5G7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Biotin transporter {ECO:0000250|UniProtKB:P0ADP5};
GN Name=bioP {ECO:0000250|UniProtKB:P0ADP5}; Synonyms=yigM;
GN OrderedLocusNames=Z5348, ECs4757;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Uptake of biotin. {ECO:0000250|UniProtKB:P0ADP5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=biotin(in) = biotin(out); Xref=Rhea:RHEA:28458,
CC ChEBI:CHEBI:57586; Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28460;
CC Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ADP5}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG59023.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB38180.1; -; Genomic_DNA.
DR PIR; C86070; C86070.
DR PIR; E91223; E91223.
DR RefSeq; NP_312784.1; NC_002695.1.
DR RefSeq; WP_001196238.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0ADP6; -.
DR SMR; P0ADP6; -.
DR STRING; 386585.gene:10367849; -.
DR EnsemblBacteria; AAG59023; AAG59023; Z5348.
DR EnsemblBacteria; BAB38180; BAB38180; ECs_4757.
DR GeneID; 66672265; -.
DR GeneID; 915147; -.
DR KEGG; ece:Z5348; -.
DR KEGG; ecs:ECs_4757; -.
DR HOGENOM; CLU_085269_0_0_6; -.
DR OMA; RFNPWAL; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR004779; CO/AA/NH_transpt.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
DR TIGRFAMs; TIGR00950; 2A78; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Biotin transporter"
FT /id="PRO_0000169664"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 3..128
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 139..274
FT /note="EamA 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 33727 MW; 2AF656D013405165 CRC64;
MALLIITTIL WAFSFSFYGE YLAGHVDSYF AVLVRVGLAA LVFLPFLRTR GNSLKTVGLY
MLVGAMQLGV MYMLSFRAYL YLTVSELLLF TVLTPLYITL IYDIMSKRRL RWGYAFSALL
AVIGAGIIRY DQVTDHFWTG LLLVQLSNIT FAIGMVGYKR LMETRPMPQH NAFAWFYLGA
FLVAVIAWFL LGNAQKMPQT TLQWGILVFL GVVASGIGYF MWNYGATQVD AGTLGIMNNM
HVPAGLLVNL AIWHQQPHWP TFITGALVIL ASLWVHRKWV APRSSQTADD RRRDCALSE
