BIOP_ECOLI
ID BIOP_ECOLI Reviewed; 299 AA.
AC P0ADP5; P27849; Q2M8D2; Q8X5G7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Biotin transporter {ECO:0000303|Ref.5};
DE AltName: Full=Plasmamembrane biotin transport protein {ECO:0000303|Ref.5};
GN Name=bioP {ECO:0000303|PubMed:1091631}; Synonyms=yigM;
GN OrderedLocusNames=b3827, JW3803;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISRUPTION PHENOTYPE, AND GENE NAME.
RX PubMed=1091631; DOI=10.1128/jb.122.1.66-72.1975;
RA Eisenburg M.A., Mee B., Prakash O., Eisenburg M.R.;
RT "Properties of alpha-dehydrobiotin-resistant mutants of Escherichia coli K-
RT 12.";
RL J. Bacteriol. 122:66-72(1975).
RN [5]
RP FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RA Ringlstetter S.L.;
RT "Identification of the biotin transporter in Escherichia coli,
RT biotinylation of histones in Saccharomyces cerevisiae and analysis of
RT biotin sensing in Saccharomyces cerevisiae.";
RL Thesis (2011), University of Regensburg, Germany.
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=24256712; DOI=10.4161/bioe.26887;
RA Finkenwirth F., Kirsch F., Eitinger T.;
RT "A versatile Escherichia coli strain for identification of biotin
RT transporters and for biotin quantification.";
RL Bioengineered 5:129-132(2014).
CC -!- FUNCTION: Uptake of biotin. Acts probably by facilitated diffusion.
CC {ECO:0000269|Ref.5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=biotin(in) = biotin(out); Xref=Rhea:RHEA:28458,
CC ChEBI:CHEBI:57586; Evidence={ECO:0000305|Ref.5};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28460;
CC Evidence={ECO:0000305|Ref.5};
CC -!- ACTIVITY REGULATION: Biotin uptake is weakly inhibited by CCCP and FCCP
CC protonophores and by the respiratory chain blocker NaN(3).
CC {ECO:0000269|Ref.5}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=74 nM for biotin {ECO:0000269|Ref.5};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|Ref.5}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is repressed by biotin. {ECO:0000269|Ref.5}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene abolishes biotin uptake
CC (Ref.5, PubMed:1091631). BioH-bioP double mutant is unable to grow on
CC trace levels of biotin (PubMed:24256712). {ECO:0000269|PubMed:1091631,
CC ECO:0000269|PubMed:24256712, ECO:0000269|Ref.5}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC {ECO:0000305}.
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DR EMBL; M87049; AAA67623.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76830.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77474.1; -; Genomic_DNA.
DR PIR; D65187; D65187.
DR RefSeq; NP_418271.1; NC_000913.3.
DR RefSeq; WP_001196238.1; NZ_STEB01000021.1.
DR AlphaFoldDB; P0ADP5; -.
DR SMR; P0ADP5; -.
DR BioGRID; 4259475; 5.
DR STRING; 511145.b3827; -.
DR TCDB; 2.A.7.3.61; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P0ADP5; -.
DR PRIDE; P0ADP5; -.
DR EnsemblBacteria; AAC76830; AAC76830; b3827.
DR EnsemblBacteria; BAE77474; BAE77474; BAE77474.
DR GeneID; 66672265; -.
DR GeneID; 948309; -.
DR KEGG; ecj:JW3803; -.
DR KEGG; eco:b3827; -.
DR PATRIC; fig|511145.12.peg.3943; -.
DR EchoBASE; EB1439; -.
DR eggNOG; COG0697; Bacteria.
DR HOGENOM; CLU_085269_0_0_6; -.
DR InParanoid; P0ADP5; -.
DR OMA; RFNPWAL; -.
DR PhylomeDB; P0ADP5; -.
DR BioCyc; EcoCyc:EG11471-MON; -.
DR BioCyc; MetaCyc:EG11471-MON; -.
DR PRO; PR:P0ADP5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR InterPro; IPR004779; CO/AA/NH_transpt.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
DR TIGRFAMs; TIGR00950; 2A78; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Biotin transporter"
FT /id="PRO_0000169663"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 3..128
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 139..274
FT /note="EamA 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 33727 MW; 2AF656D013405165 CRC64;
MALLIITTIL WAFSFSFYGE YLAGHVDSYF AVLVRVGLAA LVFLPFLRTR GNSLKTVGLY
MLVGAMQLGV MYMLSFRAYL YLTVSELLLF TVLTPLYITL IYDIMSKRRL RWGYAFSALL
AVIGAGIIRY DQVTDHFWTG LLLVQLSNIT FAIGMVGYKR LMETRPMPQH NAFAWFYLGA
FLVAVIAWFL LGNAQKMPQT TLQWGILVFL GVVASGIGYF MWNYGATQVD AGTLGIMNNM
HVPAGLLVNL AIWHQQPHWP TFITGALVIL ASLWVHRKWV APRSSQTADD RRRDCALSE
