BIOP_PSEAE
ID BIOP_PSEAE Reviewed; 286 AA.
AC O68826;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Probable biotin transporter {ECO:0000250|UniProtKB:P0ADP5};
GN OrderedLocusNames=PA3474;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Xie G., Jensen R.A.;
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Uptake of biotin. {ECO:0000250|UniProtKB:P0ADP5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=biotin(in) = biotin(out); Xref=Rhea:RHEA:28458,
CC ChEBI:CHEBI:57586; Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28460;
CC Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ADP5}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC {ECO:0000305}.
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DR EMBL; AF054868; AAC08597.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06862.1; -; Genomic_DNA.
DR PIR; G83211; G83211.
DR RefSeq; NP_252164.1; NC_002516.2.
DR RefSeq; WP_003092000.1; NZ_QZGE01000039.1.
DR AlphaFoldDB; O68826; -.
DR SMR; O68826; -.
DR STRING; 287.DR97_4447; -.
DR PaxDb; O68826; -.
DR DNASU; 878975; -.
DR EnsemblBacteria; AAG06862; AAG06862; PA3474.
DR GeneID; 878975; -.
DR KEGG; pae:PA3474; -.
DR PATRIC; fig|208964.12.peg.3637; -.
DR PseudoCAP; PA3474; -.
DR HOGENOM; CLU_085269_0_0_6; -.
DR InParanoid; O68826; -.
DR OMA; RFNPWAL; -.
DR PhylomeDB; O68826; -.
DR BioCyc; PAER208964:G1FZ6-3542-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR004779; CO/AA/NH_transpt.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
DR TIGRFAMs; TIGR00950; 2A78; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..286
FT /note="Probable biotin transporter"
FT /id="PRO_0000169667"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 3..128
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 139..277
FT /note="EamA 2"
FT /evidence="ECO:0000255"
FT CONFLICT 27
FT /note="D -> G (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 35
FT /note="R -> P (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="A -> V (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="N -> Y (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="P -> L (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="A -> V (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
FT CONFLICT 282
FT /note="R -> G (in Ref. 1; AAC08597)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 286 AA; 31835 MW; C84001C649BEE9CE CRC64;
MRYLLFVTVL WAFSFNLIGE YLAGQVDSYF AVLTRVLLAG LVFLPLTRWR GVEPRFVGGV
MLVGALQFGI TYVCLYLSFN VLTVPEVLLF TVLTPVHVAL FDDLLNRRFN FWALAAALVA
VLGAAIIRYD GITGEFLQGF LLLQLANATF AAGQVLYKRL VRKYPSELPQ RQRFGYFFVG
ALLVALPAWL LFGDPQRLPA GELQWGVLVW MGLLATALGQ FWWNKGATEV DAGTLAVMNN
LHVPVGLLLN LLIWNQHADL PRLALGGAVI VASLWVNRLG RREVRA
