ID   BIOP_SALTY              Reviewed;         299 AA.
AC   P0A1U2; Q9L6N3;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Biotin transporter {ECO:0000250|UniProtKB:P0ADP5};
GN   Name=bioP {ECO:0000250|UniProtKB:P0ADP5}; Synonyms=yigM;
GN   OrderedLocusNames=STM3963; ORFNames=STMD1.27;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Uptake of biotin. {ECO:0000250|UniProtKB:P0ADP5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=biotin(in) = biotin(out); Xref=Rhea:RHEA:28458,
CC         ChEBI:CHEBI:57586; Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28460;
CC         Evidence={ECO:0000250|UniProtKB:P0ADP5};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0ADP5}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF233324; AAF33429.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22807.1; -; Genomic_DNA.
DR   RefSeq; NP_462848.1; NC_003197.2.
DR   RefSeq; WP_001196257.1; NC_003197.2.
DR   AlphaFoldDB; P0A1U2; -.
DR   SMR; P0A1U2; -.
DR   STRING; 99287.STM3963; -.
DR   PaxDb; P0A1U2; -.
DR   EnsemblBacteria; AAL22807; AAL22807; STM3963.
DR   GeneID; 1255489; -.
DR   KEGG; stm:STM3963; -.
DR   PATRIC; fig|99287.12.peg.4181; -.
DR   HOGENOM; CLU_085269_0_0_6; -.
DR   OMA; RFNPWAL; -.
DR   PhylomeDB; P0A1U2; -.
DR   BioCyc; SENT99287:STM3963-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004779; CO/AA/NH_transpt.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
DR   TIGRFAMs; TIGR00950; 2A78; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..299
FT                   /note="Biotin transporter"
FT                   /id="PRO_0000169666"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          3..128
FT                   /note="EamA 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          139..274
FT                   /note="EamA 2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   299 AA;  33585 MW;  4D7526FEB04D5832 CRC64;
     MALLIITTIL WAFSFSLFGE YLAGHVDSYF AVLIRVGLAA LVFLPFLRTR GHNLKTISLY
     MLVGAMQLGI MYMLSFHAYL YLTVSELLLF TVLTPLYITL IYDVMSQRRL RWGYAFSALL
     AVIGAGIIRY DRVTDHFWVG LLLVQLSNIS FAIGMVGYKR LMETRPMPQH NAFAWFYLGA
     FLVAAVAWSL LGNAQKLPET TLQWSILVFL GVVASGIGYF MWNYGATQVD AGTLGIMNNM
     HVPAGLLVNL AIWHQQPHWP SFITGAAVIL ASLWVHRKWV APRSAQTADD RRRDPASSE